Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15055 and RR42_RS15060 are separated by 206 nucleotides RR42_RS15060 and RR42_RS15065 are separated by 198 nucleotides RR42_RS15065 and RR42_RS15070 are separated by 5 nucleotides RR42_RS15070 and RR42_RS15075 are separated by 10 nucleotides
RR42_RS15055: RR42_RS15055 - hypothetical protein, at 3,257,514 to 3,258,233
_RS15055
RR42_RS15060: RR42_RS15060 - membrane protein, at 3,258,440 to 3,258,874
_RS15060
RR42_RS15065: RR42_RS15065 - serine kinase, at 3,259,073 to 3,260,056
_RS15065
RR42_RS15070: RR42_RS15070 - membrane protein, at 3,260,062 to 3,260,862
_RS15070
RR42_RS15075: RR42_RS15075 - hypothetical protein, at 3,260,873 to 3,261,592
_RS15075
Position (kb)
3259
3260
3261 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3258.165 kb on + strand at 3258.165 kb on + strand at 3258.165 kb on + strand at 3258.165 kb on + strand at 3258.171 kb on + strand at 3258.174 kb on + strand at 3258.174 kb on + strand at 3258.174 kb on + strand at 3258.175 kb on - strand at 3258.226 kb on - strand at 3258.336 kb on + strand at 3258.337 kb on - strand at 3258.728 kb on + strand, within RR42_RS15060 at 3258.758 kb on + strand, within RR42_RS15060 at 3258.785 kb on + strand, within RR42_RS15060 at 3258.846 kb on + strand at 3260.069 kb on + strand at 3260.070 kb on - strand at 3260.095 kb on + strand at 3260.307 kb on - strand, within RR42_RS15070 at 3260.310 kb on - strand, within RR42_RS15070 at 3260.374 kb on + strand, within RR42_RS15070 at 3260.374 kb on + strand, within RR42_RS15070 at 3260.374 kb on + strand, within RR42_RS15070 at 3260.374 kb on + strand, within RR42_RS15070 at 3260.375 kb on - strand, within RR42_RS15070 at 3260.375 kb on - strand, within RR42_RS15070 at 3260.375 kb on - strand, within RR42_RS15070 at 3260.375 kb on - strand, within RR42_RS15070 at 3260.375 kb on - strand, within RR42_RS15070 at 3260.375 kb on - strand, within RR42_RS15070 at 3260.375 kb on - strand, within RR42_RS15070 at 3260.375 kb on - strand, within RR42_RS15070 at 3260.524 kb on - strand, within RR42_RS15070 at 3260.597 kb on + strand, within RR42_RS15070 at 3260.597 kb on + strand, within RR42_RS15070 at 3260.597 kb on + strand, within RR42_RS15070 at 3260.779 kb on + strand, within RR42_RS15070 at 3260.779 kb on + strand, within RR42_RS15070 at 3260.792 kb on - strand at 3261.014 kb on + strand, within RR42_RS15075
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 3,258,165 + +0.1 3,258,165 + +3.0 3,258,165 + -1.8 3,258,165 + +0.9 3,258,171 + +0.9 3,258,174 + +0.2 3,258,174 + -1.4 3,258,174 + +0.6 3,258,175 - -0.4 3,258,226 - +0.2 3,258,336 + -0.6 3,258,337 - -0.5 3,258,728 + RR42_RS15060 0.66 -2.3 3,258,758 + RR42_RS15060 0.73 +0.9 3,258,785 + RR42_RS15060 0.79 -0.0 3,258,846 + -0.8 3,260,069 + -1.7 3,260,070 - -2.2 3,260,095 + -2.2 3,260,307 - RR42_RS15070 0.31 -1.3 3,260,310 - RR42_RS15070 0.31 -2.8 3,260,374 + RR42_RS15070 0.39 -1.1 3,260,374 + RR42_RS15070 0.39 -1.7 3,260,374 + RR42_RS15070 0.39 -2.5 3,260,374 + RR42_RS15070 0.39 -1.8 3,260,375 - RR42_RS15070 0.39 -2.8 3,260,375 - RR42_RS15070 0.39 -2.1 3,260,375 - RR42_RS15070 0.39 -1.9 3,260,375 - RR42_RS15070 0.39 -1.6 3,260,375 - RR42_RS15070 0.39 -1.1 3,260,375 - RR42_RS15070 0.39 -2.7 3,260,375 - RR42_RS15070 0.39 -1.1 3,260,375 - RR42_RS15070 0.39 +1.0 3,260,524 - RR42_RS15070 0.58 +3.9 3,260,597 + RR42_RS15070 0.67 -2.5 3,260,597 + RR42_RS15070 0.67 -1.6 3,260,597 + RR42_RS15070 0.67 -0.9 3,260,779 + RR42_RS15070 0.90 -3.1 3,260,779 + RR42_RS15070 0.90 -2.4 3,260,792 - -1.2 3,261,014 + RR42_RS15075 0.20 +0.4
Or see this region's nucleotide sequence