Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14740 and RR42_RS14745 overlap by 4 nucleotides RR42_RS14745 and RR42_RS14750 are separated by 195 nucleotides RR42_RS14750 and RR42_RS14755 are separated by 48 nucleotides RR42_RS14755 and RR42_RS14760 are separated by 110 nucleotides
RR42_RS14740: RR42_RS14740 - thymidylate synthase, at 3,177,476 to 3,178,270
_RS14740
RR42_RS14745: RR42_RS14745 - diacylglycerol kinase, at 3,178,267 to 3,178,767
_RS14745
RR42_RS14750: RR42_RS14750 - peptidase PMbA, at 3,178,963 to 3,180,333
_RS14750
RR42_RS14755: RR42_RS14755 - hypothetical protein, at 3,180,382 to 3,181,008
_RS14755
RR42_RS14760: RR42_RS14760 - molybdenum cofactor biosynthesis protein MogA, at 3,181,119 to 3,181,709
_RS14760
Position (kb)
3178
3179
3180
3181 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3178.784 kb on + strand at 3178.784 kb on + strand at 3178.964 kb on + strand at 3178.964 kb on + strand at 3178.964 kb on + strand at 3179.136 kb on - strand, within RR42_RS14750 at 3179.172 kb on - strand, within RR42_RS14750 at 3179.172 kb on - strand, within RR42_RS14750 at 3179.172 kb on - strand, within RR42_RS14750 at 3179.172 kb on - strand, within RR42_RS14750 at 3179.172 kb on - strand, within RR42_RS14750 at 3179.336 kb on + strand, within RR42_RS14750 at 3179.336 kb on + strand, within RR42_RS14750 at 3179.337 kb on - strand, within RR42_RS14750 at 3179.351 kb on + strand, within RR42_RS14750 at 3179.351 kb on + strand, within RR42_RS14750 at 3179.351 kb on + strand, within RR42_RS14750 at 3179.359 kb on + strand, within RR42_RS14750 at 3179.360 kb on - strand, within RR42_RS14750 at 3179.360 kb on - strand, within RR42_RS14750 at 3179.360 kb on - strand, within RR42_RS14750 at 3179.467 kb on - strand, within RR42_RS14750 at 3179.467 kb on - strand, within RR42_RS14750 at 3179.467 kb on - strand, within RR42_RS14750 at 3179.467 kb on - strand, within RR42_RS14750 at 3179.502 kb on - strand, within RR42_RS14750 at 3179.657 kb on + strand, within RR42_RS14750 at 3179.787 kb on - strand, within RR42_RS14750 at 3179.848 kb on - strand, within RR42_RS14750 at 3180.042 kb on - strand, within RR42_RS14750 at 3180.042 kb on - strand, within RR42_RS14750 at 3180.137 kb on - strand, within RR42_RS14750 at 3180.137 kb on - strand, within RR42_RS14750 at 3180.295 kb on - strand at 3180.296 kb on + strand at 3180.296 kb on + strand at 3180.296 kb on + strand at 3180.296 kb on + strand at 3180.297 kb on - strand at 3180.297 kb on - strand at 3180.297 kb on - strand at 3180.370 kb on + strand at 3180.370 kb on + strand at 3180.371 kb on - strand at 3180.371 kb on - strand at 3180.378 kb on + strand at 3180.508 kb on - strand, within RR42_RS14755 at 3180.655 kb on + strand, within RR42_RS14755 at 3180.655 kb on + strand, within RR42_RS14755 at 3180.655 kb on + strand, within RR42_RS14755 at 3180.655 kb on + strand, within RR42_RS14755 at 3180.656 kb on - strand, within RR42_RS14755 at 3180.934 kb on - strand, within RR42_RS14755 at 3181.123 kb on + strand at 3181.124 kb on - strand at 3181.164 kb on + strand at 3181.165 kb on - strand at 3181.165 kb on - strand at 3181.165 kb on - strand at 3181.214 kb on + strand, within RR42_RS14760 at 3181.215 kb on - strand, within RR42_RS14760
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 3,178,784 + +1.0 3,178,784 + +1.0 3,178,964 + +0.2 3,178,964 + -0.2 3,178,964 + -0.2 3,179,136 - RR42_RS14750 0.13 -0.2 3,179,172 - RR42_RS14750 0.15 -2.0 3,179,172 - RR42_RS14750 0.15 +1.3 3,179,172 - RR42_RS14750 0.15 -0.6 3,179,172 - RR42_RS14750 0.15 +0.7 3,179,172 - RR42_RS14750 0.15 +0.7 3,179,336 + RR42_RS14750 0.27 -1.5 3,179,336 + RR42_RS14750 0.27 -0.6 3,179,337 - RR42_RS14750 0.27 -0.4 3,179,351 + RR42_RS14750 0.28 +0.0 3,179,351 + RR42_RS14750 0.28 +0.8 3,179,351 + RR42_RS14750 0.28 -0.2 3,179,359 + RR42_RS14750 0.29 -1.0 3,179,360 - RR42_RS14750 0.29 +0.3 3,179,360 - RR42_RS14750 0.29 +0.1 3,179,360 - RR42_RS14750 0.29 -1.9 3,179,467 - RR42_RS14750 0.37 +0.5 3,179,467 - RR42_RS14750 0.37 -0.7 3,179,467 - RR42_RS14750 0.37 -0.0 3,179,467 - RR42_RS14750 0.37 -0.2 3,179,502 - RR42_RS14750 0.39 -0.9 3,179,657 + RR42_RS14750 0.51 -2.0 3,179,787 - RR42_RS14750 0.60 +0.0 3,179,848 - RR42_RS14750 0.65 -0.3 3,180,042 - RR42_RS14750 0.79 +0.7 3,180,042 - RR42_RS14750 0.79 +0.2 3,180,137 - RR42_RS14750 0.86 -0.2 3,180,137 - RR42_RS14750 0.86 -0.1 3,180,295 - +0.3 3,180,296 + -0.9 3,180,296 + -0.2 3,180,296 + -0.3 3,180,296 + -0.1 3,180,297 - -1.5 3,180,297 - +1.2 3,180,297 - -0.3 3,180,370 + -0.7 3,180,370 + +0.2 3,180,371 - -1.7 3,180,371 - +0.8 3,180,378 + -2.4 3,180,508 - RR42_RS14755 0.20 +0.2 3,180,655 + RR42_RS14755 0.44 -0.1 3,180,655 + RR42_RS14755 0.44 -1.1 3,180,655 + RR42_RS14755 0.44 +0.9 3,180,655 + RR42_RS14755 0.44 +0.5 3,180,656 - RR42_RS14755 0.44 -2.4 3,180,934 - RR42_RS14755 0.88 -0.1 3,181,123 + -3.8 3,181,124 - -1.9 3,181,164 + +0.5 3,181,165 - -0.7 3,181,165 - -1.9 3,181,165 - -2.1 3,181,214 + RR42_RS14760 0.16 -0.2 3,181,215 - RR42_RS14760 0.16 -1.3
Or see this region's nucleotide sequence