Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS14035

Experiment: R2A with Cobalt chloride 0.3 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS14025 and RR42_RS14030 are separated by 30 nucleotidesRR42_RS14030 and RR42_RS14035 overlap by 8 nucleotidesRR42_RS14035 and RR42_RS14040 overlap by 4 nucleotidesRR42_RS14040 and RR42_RS14045 are separated by 1 nucleotides RR42_RS14025: RR42_RS14025 - pyridoxine 5'-phosphate synthase, at 3,019,647 to 3,020,429 _RS14025 RR42_RS14030: RR42_RS14030 - DNA recombination protein RecO, at 3,020,460 to 3,021,338 _RS14030 RR42_RS14035: RR42_RS14035 - GTPase Era, at 3,021,331 to 3,022,266 _RS14035 RR42_RS14040: RR42_RS14040 - ribonuclease III, at 3,022,263 to 3,023,033 _RS14040 RR42_RS14045: RR42_RS14045 - membrane protein, at 3,023,035 to 3,023,391 _RS14045 Position (kb) 3021 3022 3023Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3020.443 kb on - strandat 3020.457 kb on - strandat 3020.519 kb on - strandat 3020.577 kb on - strand, within RR42_RS14030at 3020.578 kb on + strand, within RR42_RS14030at 3020.579 kb on - strand, within RR42_RS14030at 3020.677 kb on + strand, within RR42_RS14030at 3020.683 kb on + strand, within RR42_RS14030at 3020.683 kb on + strand, within RR42_RS14030at 3020.834 kb on - strand, within RR42_RS14030at 3020.891 kb on - strand, within RR42_RS14030at 3021.024 kb on + strand, within RR42_RS14030at 3023.055 kb on - strandat 3023.059 kb on - strandat 3023.082 kb on - strand, within RR42_RS14045at 3023.082 kb on - strand, within RR42_RS14045at 3023.084 kb on - strand, within RR42_RS14045at 3023.179 kb on - strand, within RR42_RS14045

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM
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3,020,443 - +0.8
3,020,457 - -0.2
3,020,519 - -0.5
3,020,577 - RR42_RS14030 0.13 +0.5
3,020,578 + RR42_RS14030 0.13 -0.2
3,020,579 - RR42_RS14030 0.14 -0.3
3,020,677 + RR42_RS14030 0.25 -0.3
3,020,683 + RR42_RS14030 0.25 +0.4
3,020,683 + RR42_RS14030 0.25 +0.4
3,020,834 - RR42_RS14030 0.43 -1.8
3,020,891 - RR42_RS14030 0.49 +1.3
3,021,024 + RR42_RS14030 0.64 +0.4
3,023,055 - +1.0
3,023,059 - +0.8
3,023,082 - RR42_RS14045 0.13 -0.5
3,023,082 - RR42_RS14045 0.13 -1.8
3,023,084 - RR42_RS14045 0.14 +0.4
3,023,179 - RR42_RS14045 0.40 -3.9

Or see this region's nucleotide sequence