Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14025 and RR42_RS14030 are separated by 30 nucleotides RR42_RS14030 and RR42_RS14035 overlap by 8 nucleotides RR42_RS14035 and RR42_RS14040 overlap by 4 nucleotides RR42_RS14040 and RR42_RS14045 are separated by 1 nucleotides
RR42_RS14025: RR42_RS14025 - pyridoxine 5'-phosphate synthase, at 3,019,647 to 3,020,429
_RS14025
RR42_RS14030: RR42_RS14030 - DNA recombination protein RecO, at 3,020,460 to 3,021,338
_RS14030
RR42_RS14035: RR42_RS14035 - GTPase Era, at 3,021,331 to 3,022,266
_RS14035
RR42_RS14040: RR42_RS14040 - ribonuclease III, at 3,022,263 to 3,023,033
_RS14040
RR42_RS14045: RR42_RS14045 - membrane protein, at 3,023,035 to 3,023,391
_RS14045
Position (kb)
3021
3022
3023 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3020.443 kb on - strand at 3020.457 kb on - strand at 3020.519 kb on - strand at 3020.577 kb on - strand, within RR42_RS14030 at 3020.578 kb on + strand, within RR42_RS14030 at 3020.579 kb on - strand, within RR42_RS14030 at 3020.677 kb on + strand, within RR42_RS14030 at 3020.683 kb on + strand, within RR42_RS14030 at 3020.683 kb on + strand, within RR42_RS14030 at 3020.834 kb on - strand, within RR42_RS14030 at 3020.891 kb on - strand, within RR42_RS14030 at 3021.024 kb on + strand, within RR42_RS14030 at 3023.055 kb on - strand at 3023.059 kb on - strand at 3023.082 kb on - strand, within RR42_RS14045 at 3023.082 kb on - strand, within RR42_RS14045 at 3023.084 kb on - strand, within RR42_RS14045 at 3023.179 kb on - strand, within RR42_RS14045
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 3,020,443 - +0.8 3,020,457 - -0.2 3,020,519 - -0.5 3,020,577 - RR42_RS14030 0.13 +0.5 3,020,578 + RR42_RS14030 0.13 -0.2 3,020,579 - RR42_RS14030 0.14 -0.3 3,020,677 + RR42_RS14030 0.25 -0.3 3,020,683 + RR42_RS14030 0.25 +0.4 3,020,683 + RR42_RS14030 0.25 +0.4 3,020,834 - RR42_RS14030 0.43 -1.8 3,020,891 - RR42_RS14030 0.49 +1.3 3,021,024 + RR42_RS14030 0.64 +0.4 3,023,055 - +1.0 3,023,059 - +0.8 3,023,082 - RR42_RS14045 0.13 -0.5 3,023,082 - RR42_RS14045 0.13 -1.8 3,023,084 - RR42_RS14045 0.14 +0.4 3,023,179 - RR42_RS14045 0.40 -3.9
Or see this region's nucleotide sequence