Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS13735

Experiment: R2A with Cobalt chloride 0.3 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS13725 and RR42_RS13730 are separated by 57 nucleotidesRR42_RS13730 and RR42_RS13735 are separated by 252 nucleotidesRR42_RS13735 and RR42_RS13740 are separated by 152 nucleotidesRR42_RS13740 and RR42_RS13745 are separated by 139 nucleotides RR42_RS13725: RR42_RS13725 - membrane protein, at 2,962,376 to 2,963,602 _RS13725 RR42_RS13730: RR42_RS13730 - TetR family transcriptional regulator, at 2,963,660 to 2,964,274 _RS13730 RR42_RS13735: RR42_RS13735 - ADP-heptose--LPS heptosyltransferase, at 2,964,527 to 2,965,525 _RS13735 RR42_RS13740: RR42_RS13740 - isochorismatase, at 2,965,678 to 2,966,283 _RS13740 RR42_RS13745: RR42_RS13745 - LysR family transcriptional regulator, at 2,966,423 to 2,967,295 _RS13745 Position (kb) 2964 2965 2966Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 2963.614 kb on - strandat 2963.614 kb on - strandat 2963.614 kb on - strandat 2963.619 kb on + strandat 2963.619 kb on + strandat 2963.619 kb on + strandat 2963.669 kb on - strandat 2963.708 kb on - strandat 2964.090 kb on + strand, within RR42_RS13730at 2964.142 kb on - strand, within RR42_RS13730at 2964.142 kb on - strand, within RR42_RS13730at 2964.315 kb on + strandat 2964.317 kb on + strandat 2964.659 kb on - strand, within RR42_RS13735at 2964.895 kb on - strand, within RR42_RS13735at 2964.895 kb on - strand, within RR42_RS13735at 2965.065 kb on + strand, within RR42_RS13735at 2965.066 kb on - strand, within RR42_RS13735at 2965.237 kb on - strand, within RR42_RS13735at 2965.237 kb on - strand, within RR42_RS13735at 2965.237 kb on - strand, within RR42_RS13735at 2965.266 kb on + strand, within RR42_RS13735at 2965.267 kb on - strand, within RR42_RS13735at 2965.393 kb on + strand, within RR42_RS13735at 2965.441 kb on - strandat 2965.483 kb on + strandat 2965.484 kb on - strandat 2965.577 kb on + strandat 2965.577 kb on + strandat 2965.577 kb on + strandat 2965.577 kb on + strandat 2965.578 kb on - strandat 2965.580 kb on + strandat 2965.580 kb on + strandat 2965.580 kb on + strandat 2965.580 kb on + strandat 2965.581 kb on - strandat 2965.581 kb on - strandat 2965.581 kb on - strandat 2965.878 kb on + strand, within RR42_RS13740at 2965.878 kb on + strand, within RR42_RS13740at 2965.878 kb on + strand, within RR42_RS13740at 2965.879 kb on - strand, within RR42_RS13740at 2965.879 kb on - strand, within RR42_RS13740at 2965.879 kb on - strand, within RR42_RS13740at 2965.879 kb on - strand, within RR42_RS13740at 2965.879 kb on - strand, within RR42_RS13740at 2965.918 kb on + strand, within RR42_RS13740at 2966.183 kb on + strand, within RR42_RS13740at 2966.184 kb on - strand, within RR42_RS13740at 2966.268 kb on + strandat 2966.300 kb on + strandat 2966.300 kb on + strandat 2966.300 kb on + strandat 2966.300 kb on + strandat 2966.300 kb on + strandat 2966.358 kb on + strandat 2966.376 kb on - strandat 2966.490 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM
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2,963,614 - +0.5
2,963,614 - -0.3
2,963,614 - -0.8
2,963,619 + +0.6
2,963,619 + +0.5
2,963,619 + +0.5
2,963,669 - +1.8
2,963,708 - +3.0
2,964,090 + RR42_RS13730 0.70 +0.0
2,964,142 - RR42_RS13730 0.78 +0.7
2,964,142 - RR42_RS13730 0.78 -0.4
2,964,315 + +1.3
2,964,317 + +0.6
2,964,659 - RR42_RS13735 0.13 +3.2
2,964,895 - RR42_RS13735 0.37 +1.1
2,964,895 - RR42_RS13735 0.37 +3.2
2,965,065 + RR42_RS13735 0.54 +2.9
2,965,066 - RR42_RS13735 0.54 +2.8
2,965,237 - RR42_RS13735 0.71 +2.3
2,965,237 - RR42_RS13735 0.71 +1.2
2,965,237 - RR42_RS13735 0.71 +2.8
2,965,266 + RR42_RS13735 0.74 +2.3
2,965,267 - RR42_RS13735 0.74 +2.9
2,965,393 + RR42_RS13735 0.87 +3.6
2,965,441 - +0.5
2,965,483 + +2.7
2,965,484 - +3.0
2,965,577 + +3.7
2,965,577 + +2.9
2,965,577 + -0.2
2,965,577 + +0.5
2,965,578 - -0.6
2,965,580 + +3.3
2,965,580 + +1.1
2,965,580 + +3.0
2,965,580 + +3.3
2,965,581 - -0.4
2,965,581 - -4.2
2,965,581 - -0.8
2,965,878 + RR42_RS13740 0.33 +0.7
2,965,878 + RR42_RS13740 0.33 -1.6
2,965,878 + RR42_RS13740 0.33 +0.4
2,965,879 - RR42_RS13740 0.33 -0.8
2,965,879 - RR42_RS13740 0.33 -0.3
2,965,879 - RR42_RS13740 0.33 -1.3
2,965,879 - RR42_RS13740 0.33 -0.4
2,965,879 - RR42_RS13740 0.33 -1.4
2,965,918 + RR42_RS13740 0.40 +0.3
2,966,183 + RR42_RS13740 0.83 +0.5
2,966,184 - RR42_RS13740 0.83 -1.0
2,966,268 + +0.1
2,966,300 + -0.3
2,966,300 + +0.2
2,966,300 + +1.0
2,966,300 + -0.7
2,966,300 + +0.7
2,966,358 + -1.7
2,966,376 - +0.9
2,966,490 - -0.3

Or see this region's nucleotide sequence