Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS13725 and RR42_RS13730 are separated by 57 nucleotides RR42_RS13730 and RR42_RS13735 are separated by 252 nucleotides RR42_RS13735 and RR42_RS13740 are separated by 152 nucleotides RR42_RS13740 and RR42_RS13745 are separated by 139 nucleotides
RR42_RS13725: RR42_RS13725 - membrane protein, at 2,962,376 to 2,963,602
_RS13725
RR42_RS13730: RR42_RS13730 - TetR family transcriptional regulator, at 2,963,660 to 2,964,274
_RS13730
RR42_RS13735: RR42_RS13735 - ADP-heptose--LPS heptosyltransferase, at 2,964,527 to 2,965,525
_RS13735
RR42_RS13740: RR42_RS13740 - isochorismatase, at 2,965,678 to 2,966,283
_RS13740
RR42_RS13745: RR42_RS13745 - LysR family transcriptional regulator, at 2,966,423 to 2,967,295
_RS13745
Position (kb)
2964
2965
2966 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2963.614 kb on - strand at 2963.614 kb on - strand at 2963.614 kb on - strand at 2963.619 kb on + strand at 2963.619 kb on + strand at 2963.619 kb on + strand at 2963.669 kb on - strand at 2963.708 kb on - strand at 2964.090 kb on + strand, within RR42_RS13730 at 2964.142 kb on - strand, within RR42_RS13730 at 2964.142 kb on - strand, within RR42_RS13730 at 2964.315 kb on + strand at 2964.317 kb on + strand at 2964.659 kb on - strand, within RR42_RS13735 at 2964.895 kb on - strand, within RR42_RS13735 at 2964.895 kb on - strand, within RR42_RS13735 at 2965.065 kb on + strand, within RR42_RS13735 at 2965.066 kb on - strand, within RR42_RS13735 at 2965.237 kb on - strand, within RR42_RS13735 at 2965.237 kb on - strand, within RR42_RS13735 at 2965.237 kb on - strand, within RR42_RS13735 at 2965.266 kb on + strand, within RR42_RS13735 at 2965.267 kb on - strand, within RR42_RS13735 at 2965.393 kb on + strand, within RR42_RS13735 at 2965.441 kb on - strand at 2965.483 kb on + strand at 2965.484 kb on - strand at 2965.577 kb on + strand at 2965.577 kb on + strand at 2965.577 kb on + strand at 2965.577 kb on + strand at 2965.578 kb on - strand at 2965.580 kb on + strand at 2965.580 kb on + strand at 2965.580 kb on + strand at 2965.580 kb on + strand at 2965.581 kb on - strand at 2965.581 kb on - strand at 2965.581 kb on - strand at 2965.878 kb on + strand, within RR42_RS13740 at 2965.878 kb on + strand, within RR42_RS13740 at 2965.878 kb on + strand, within RR42_RS13740 at 2965.879 kb on - strand, within RR42_RS13740 at 2965.879 kb on - strand, within RR42_RS13740 at 2965.879 kb on - strand, within RR42_RS13740 at 2965.879 kb on - strand, within RR42_RS13740 at 2965.879 kb on - strand, within RR42_RS13740 at 2965.918 kb on + strand, within RR42_RS13740 at 2966.183 kb on + strand, within RR42_RS13740 at 2966.184 kb on - strand, within RR42_RS13740 at 2966.268 kb on + strand at 2966.300 kb on + strand at 2966.300 kb on + strand at 2966.300 kb on + strand at 2966.300 kb on + strand at 2966.300 kb on + strand at 2966.358 kb on + strand at 2966.376 kb on - strand at 2966.490 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 2,963,614 - +0.5 2,963,614 - -0.3 2,963,614 - -0.8 2,963,619 + +0.6 2,963,619 + +0.5 2,963,619 + +0.5 2,963,669 - +1.8 2,963,708 - +3.0 2,964,090 + RR42_RS13730 0.70 +0.0 2,964,142 - RR42_RS13730 0.78 +0.7 2,964,142 - RR42_RS13730 0.78 -0.4 2,964,315 + +1.3 2,964,317 + +0.6 2,964,659 - RR42_RS13735 0.13 +3.2 2,964,895 - RR42_RS13735 0.37 +1.1 2,964,895 - RR42_RS13735 0.37 +3.2 2,965,065 + RR42_RS13735 0.54 +2.9 2,965,066 - RR42_RS13735 0.54 +2.8 2,965,237 - RR42_RS13735 0.71 +2.3 2,965,237 - RR42_RS13735 0.71 +1.2 2,965,237 - RR42_RS13735 0.71 +2.8 2,965,266 + RR42_RS13735 0.74 +2.3 2,965,267 - RR42_RS13735 0.74 +2.9 2,965,393 + RR42_RS13735 0.87 +3.6 2,965,441 - +0.5 2,965,483 + +2.7 2,965,484 - +3.0 2,965,577 + +3.7 2,965,577 + +2.9 2,965,577 + -0.2 2,965,577 + +0.5 2,965,578 - -0.6 2,965,580 + +3.3 2,965,580 + +1.1 2,965,580 + +3.0 2,965,580 + +3.3 2,965,581 - -0.4 2,965,581 - -4.2 2,965,581 - -0.8 2,965,878 + RR42_RS13740 0.33 +0.7 2,965,878 + RR42_RS13740 0.33 -1.6 2,965,878 + RR42_RS13740 0.33 +0.4 2,965,879 - RR42_RS13740 0.33 -0.8 2,965,879 - RR42_RS13740 0.33 -0.3 2,965,879 - RR42_RS13740 0.33 -1.3 2,965,879 - RR42_RS13740 0.33 -0.4 2,965,879 - RR42_RS13740 0.33 -1.4 2,965,918 + RR42_RS13740 0.40 +0.3 2,966,183 + RR42_RS13740 0.83 +0.5 2,966,184 - RR42_RS13740 0.83 -1.0 2,966,268 + +0.1 2,966,300 + -0.3 2,966,300 + +0.2 2,966,300 + +1.0 2,966,300 + -0.7 2,966,300 + +0.7 2,966,358 + -1.7 2,966,376 - +0.9 2,966,490 - -0.3
Or see this region's nucleotide sequence