Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12725 and RR42_RS12730 are separated by 216 nucleotides RR42_RS12730 and RR42_RS12735 are separated by 201 nucleotides RR42_RS12735 and RR42_RS12740 are separated by 58 nucleotides RR42_RS12740 and RR42_RS12745 are separated by 58 nucleotides
RR42_RS12725: RR42_RS12725 - hypothetical protein, at 2,732,431 to 2,735,028
_RS12725
RR42_RS12730: RR42_RS12730 - membrane protein, at 2,735,245 to 2,736,750
_RS12730
RR42_RS12735: RR42_RS12735 - hypothetical protein, at 2,736,952 to 2,737,212
_RS12735
RR42_RS12740: RR42_RS12740 - hypothetical protein, at 2,737,271 to 2,737,528
_RS12740
RR42_RS12745: RR42_RS12745 - hypothetical protein, at 2,737,587 to 2,737,844
_RS12745
Position (kb)
2735
2736
2737 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2734.321 kb on + strand, within RR42_RS12725 at 2734.321 kb on + strand, within RR42_RS12725 at 2734.321 kb on + strand, within RR42_RS12725 at 2734.499 kb on + strand, within RR42_RS12725 at 2734.499 kb on + strand, within RR42_RS12725 at 2734.500 kb on - strand, within RR42_RS12725 at 2734.500 kb on - strand, within RR42_RS12725 at 2734.530 kb on - strand, within RR42_RS12725 at 2734.530 kb on - strand, within RR42_RS12725 at 2734.530 kb on - strand, within RR42_RS12725 at 2734.689 kb on - strand, within RR42_RS12725 at 2734.801 kb on - strand at 2734.802 kb on + strand at 2734.802 kb on + strand at 2734.802 kb on + strand at 2734.803 kb on - strand at 2735.146 kb on + strand at 2735.146 kb on + strand at 2735.147 kb on - strand at 2735.147 kb on - strand at 2735.147 kb on - strand at 2735.147 kb on - strand at 2735.147 kb on - strand at 2735.149 kb on + strand at 2735.150 kb on - strand at 2735.190 kb on - strand at 2735.324 kb on + strand at 2735.410 kb on + strand, within RR42_RS12730 at 2735.563 kb on - strand, within RR42_RS12730 at 2736.071 kb on + strand, within RR42_RS12730 at 2736.072 kb on - strand, within RR42_RS12730 at 2736.142 kb on + strand, within RR42_RS12730 at 2736.264 kb on + strand, within RR42_RS12730 at 2736.299 kb on + strand, within RR42_RS12730 at 2736.299 kb on + strand, within RR42_RS12730 at 2736.440 kb on + strand, within RR42_RS12730 at 2736.890 kb on + strand at 2736.891 kb on - strand at 2736.891 kb on - strand at 2736.959 kb on + strand at 2736.959 kb on + strand at 2736.960 kb on - strand at 2737.275 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 2,734,321 + RR42_RS12725 0.73 -0.9 2,734,321 + RR42_RS12725 0.73 -0.4 2,734,321 + RR42_RS12725 0.73 +0.6 2,734,499 + RR42_RS12725 0.80 +0.3 2,734,499 + RR42_RS12725 0.80 +0.8 2,734,500 - RR42_RS12725 0.80 +0.3 2,734,500 - RR42_RS12725 0.80 +0.3 2,734,530 - RR42_RS12725 0.81 +1.2 2,734,530 - RR42_RS12725 0.81 +0.7 2,734,530 - RR42_RS12725 0.81 +0.8 2,734,689 - RR42_RS12725 0.87 +0.3 2,734,801 - -0.1 2,734,802 + -1.3 2,734,802 + +0.6 2,734,802 + -0.4 2,734,803 - +0.6 2,735,146 + +1.2 2,735,146 + -0.9 2,735,147 - +0.3 2,735,147 - +1.2 2,735,147 - +0.4 2,735,147 - +0.9 2,735,147 - -0.5 2,735,149 + -0.6 2,735,150 - -0.4 2,735,190 - -1.1 2,735,324 + +0.0 2,735,410 + RR42_RS12730 0.11 +2.9 2,735,563 - RR42_RS12730 0.21 -0.1 2,736,071 + RR42_RS12730 0.55 -0.6 2,736,072 - RR42_RS12730 0.55 +0.2 2,736,142 + RR42_RS12730 0.60 +1.2 2,736,264 + RR42_RS12730 0.68 +1.1 2,736,299 + RR42_RS12730 0.70 +1.1 2,736,299 + RR42_RS12730 0.70 +1.1 2,736,440 + RR42_RS12730 0.79 +1.0 2,736,890 + +1.4 2,736,891 - +1.7 2,736,891 - +1.4 2,736,959 + +0.5 2,736,959 + +0.6 2,736,960 - -1.2 2,737,275 + -0.8
Or see this region's nucleotide sequence