Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11640 and RR42_RS11645 are separated by 174 nucleotides RR42_RS11645 and RR42_RS11650 are separated by 176 nucleotides RR42_RS11650 and RR42_RS11655 are separated by 155 nucleotides RR42_RS11655 and RR42_RS11660 are separated by 199 nucleotides
RR42_RS11640: RR42_RS11640 - hypothetical protein, at 2,494,593 to 2,495,000
_RS11640
RR42_RS11645: RR42_RS11645 - membrane protein, at 2,495,175 to 2,495,345
_RS11645
RR42_RS11650: RR42_RS11650 - poly-beta-hydroxybutyrate polymerase, at 2,495,522 to 2,497,309
_RS11650
RR42_RS11655: RR42_RS11655 - heat-shock protein Hsp20, at 2,497,465 to 2,497,887
_RS11655
RR42_RS11660: RR42_RS11660 - hypothetical protein, at 2,498,087 to 2,498,269
_RS11660
Position (kb)
2495
2496
2497
2498 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2494.596 kb on + strand at 2494.619 kb on - strand at 2494.619 kb on - strand at 2494.641 kb on + strand, within RR42_RS11640 at 2494.722 kb on + strand, within RR42_RS11640 at 2494.823 kb on - strand, within RR42_RS11640 at 2494.823 kb on - strand, within RR42_RS11640 at 2494.824 kb on + strand, within RR42_RS11640 at 2494.824 kb on + strand, within RR42_RS11640 at 2494.825 kb on - strand, within RR42_RS11640 at 2494.825 kb on - strand, within RR42_RS11640 at 2494.848 kb on + strand, within RR42_RS11640 at 2494.849 kb on - strand, within RR42_RS11640 at 2494.850 kb on + strand, within RR42_RS11640 at 2494.851 kb on - strand, within RR42_RS11640 at 2494.851 kb on - strand, within RR42_RS11640 at 2494.851 kb on - strand, within RR42_RS11640 at 2494.851 kb on - strand, within RR42_RS11640 at 2494.920 kb on + strand, within RR42_RS11640 at 2494.921 kb on - strand, within RR42_RS11640 at 2494.921 kb on - strand, within RR42_RS11640 at 2494.966 kb on - strand at 2495.027 kb on + strand at 2495.043 kb on + strand at 2495.043 kb on + strand at 2495.044 kb on - strand at 2495.044 kb on - strand at 2495.061 kb on + strand at 2495.062 kb on - strand at 2495.062 kb on - strand at 2495.090 kb on + strand at 2495.090 kb on + strand at 2495.090 kb on + strand at 2495.091 kb on - strand at 2495.093 kb on - strand at 2495.150 kb on - strand at 2495.157 kb on + strand at 2495.157 kb on + strand at 2495.629 kb on + strand at 2495.630 kb on - strand at 2496.107 kb on - strand, within RR42_RS11650 at 2496.172 kb on - strand, within RR42_RS11650 at 2496.262 kb on - strand, within RR42_RS11650 at 2496.305 kb on + strand, within RR42_RS11650 at 2496.305 kb on + strand, within RR42_RS11650 at 2496.305 kb on + strand, within RR42_RS11650 at 2496.309 kb on - strand, within RR42_RS11650 at 2496.309 kb on - strand, within RR42_RS11650 at 2496.309 kb on - strand, within RR42_RS11650 at 2496.309 kb on - strand, within RR42_RS11650 at 2496.309 kb on - strand, within RR42_RS11650 at 2496.431 kb on + strand, within RR42_RS11650 at 2496.493 kb on + strand, within RR42_RS11650 at 2496.493 kb on + strand, within RR42_RS11650 at 2496.510 kb on - strand, within RR42_RS11650 at 2496.695 kb on + strand, within RR42_RS11650 at 2496.695 kb on + strand, within RR42_RS11650 at 2496.776 kb on + strand, within RR42_RS11650 at 2496.908 kb on + strand, within RR42_RS11650 at 2496.909 kb on - strand, within RR42_RS11650 at 2497.007 kb on + strand, within RR42_RS11650 at 2497.142 kb on + strand at 2497.143 kb on - strand at 2497.143 kb on - strand at 2497.160 kb on + strand at 2497.293 kb on - strand at 2497.539 kb on - strand, within RR42_RS11655 at 2497.719 kb on + strand, within RR42_RS11655 at 2497.869 kb on - strand at 2498.168 kb on + strand, within RR42_RS11660 at 2498.169 kb on - strand, within RR42_RS11660 at 2498.231 kb on + strand, within RR42_RS11660
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 2,494,596 + +0.0 2,494,619 - +0.3 2,494,619 - +0.4 2,494,641 + RR42_RS11640 0.12 -0.6 2,494,722 + RR42_RS11640 0.32 +0.5 2,494,823 - RR42_RS11640 0.56 -1.1 2,494,823 - RR42_RS11640 0.56 -0.0 2,494,824 + RR42_RS11640 0.57 -0.3 2,494,824 + RR42_RS11640 0.57 -1.3 2,494,825 - RR42_RS11640 0.57 -0.1 2,494,825 - RR42_RS11640 0.57 -2.0 2,494,848 + RR42_RS11640 0.62 +0.6 2,494,849 - RR42_RS11640 0.63 +1.0 2,494,850 + RR42_RS11640 0.63 -1.0 2,494,851 - RR42_RS11640 0.63 +0.4 2,494,851 - RR42_RS11640 0.63 +2.5 2,494,851 - RR42_RS11640 0.63 +0.1 2,494,851 - RR42_RS11640 0.63 -0.6 2,494,920 + RR42_RS11640 0.80 +0.2 2,494,921 - RR42_RS11640 0.80 +0.9 2,494,921 - RR42_RS11640 0.80 +0.6 2,494,966 - -0.6 2,495,027 + -0.6 2,495,043 + +0.1 2,495,043 + +0.7 2,495,044 - +1.2 2,495,044 - -2.0 2,495,061 + +0.3 2,495,062 - +0.6 2,495,062 - +0.8 2,495,090 + -1.5 2,495,090 + +0.2 2,495,090 + +0.8 2,495,091 - -0.3 2,495,093 - +0.5 2,495,150 - +0.1 2,495,157 + +0.2 2,495,157 + -0.3 2,495,629 + +0.3 2,495,630 - -0.1 2,496,107 - RR42_RS11650 0.33 -0.3 2,496,172 - RR42_RS11650 0.36 +1.0 2,496,262 - RR42_RS11650 0.41 -0.3 2,496,305 + RR42_RS11650 0.44 -0.8 2,496,305 + RR42_RS11650 0.44 +0.2 2,496,305 + RR42_RS11650 0.44 +0.3 2,496,309 - RR42_RS11650 0.44 +0.6 2,496,309 - RR42_RS11650 0.44 -0.3 2,496,309 - RR42_RS11650 0.44 -0.9 2,496,309 - RR42_RS11650 0.44 -0.3 2,496,309 - RR42_RS11650 0.44 -0.1 2,496,431 + RR42_RS11650 0.51 +0.1 2,496,493 + RR42_RS11650 0.54 +1.4 2,496,493 + RR42_RS11650 0.54 -2.8 2,496,510 - RR42_RS11650 0.55 -1.0 2,496,695 + RR42_RS11650 0.66 +1.7 2,496,695 + RR42_RS11650 0.66 +0.0 2,496,776 + RR42_RS11650 0.70 -1.1 2,496,908 + RR42_RS11650 0.78 -1.0 2,496,909 - RR42_RS11650 0.78 +2.6 2,497,007 + RR42_RS11650 0.83 +0.4 2,497,142 + -0.3 2,497,143 - -0.1 2,497,143 - +0.8 2,497,160 + -0.5 2,497,293 - +0.4 2,497,539 - RR42_RS11655 0.17 -0.4 2,497,719 + RR42_RS11655 0.60 -0.7 2,497,869 - +0.4 2,498,168 + RR42_RS11660 0.44 +0.9 2,498,169 - RR42_RS11660 0.45 +0.0 2,498,231 + RR42_RS11660 0.79 -1.3
Or see this region's nucleotide sequence