Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11405 and RR42_RS11410 are separated by 210 nucleotides RR42_RS11410 and RR42_RS11415 are separated by 165 nucleotides RR42_RS11415 and RR42_RS11420 are separated by 278 nucleotides
RR42_RS11405: RR42_RS11405 - hypothetical protein, at 2,442,133 to 2,442,639
_RS11405
RR42_RS11410: RR42_RS11410 - hypothetical protein, at 2,442,850 to 2,443,251
_RS11410
RR42_RS11415: RR42_RS11415 - hypothetical protein, at 2,443,417 to 2,443,656
_RS11415
RR42_RS11420: RR42_RS11420 - patatin, at 2,443,935 to 2,444,945
_RS11420
Position (kb)
2443
2444 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2442.617 kb on - strand at 2442.623 kb on + strand at 2442.623 kb on + strand at 2442.624 kb on - strand at 2442.624 kb on - strand at 2442.624 kb on - strand at 2442.624 kb on - strand at 2442.803 kb on + strand at 2442.803 kb on + strand at 2442.804 kb on - strand at 2442.808 kb on + strand at 2442.809 kb on - strand at 2443.206 kb on - strand, within RR42_RS11410 at 2443.326 kb on + strand at 2443.458 kb on + strand, within RR42_RS11415 at 2443.458 kb on + strand, within RR42_RS11415 at 2443.459 kb on - strand, within RR42_RS11415 at 2443.459 kb on - strand, within RR42_RS11415 at 2443.459 kb on - strand, within RR42_RS11415 at 2443.565 kb on - strand, within RR42_RS11415 at 2443.656 kb on + strand at 2443.657 kb on - strand at 2443.657 kb on - strand at 2443.680 kb on + strand at 2443.681 kb on - strand at 2443.804 kb on - strand at 2443.804 kb on - strand at 2443.804 kb on - strand at 2443.904 kb on + strand at 2443.936 kb on + strand at 2443.936 kb on + strand at 2443.963 kb on + strand at 2444.062 kb on - strand, within RR42_RS11420 at 2444.062 kb on - strand, within RR42_RS11420 at 2444.194 kb on - strand, within RR42_RS11420 at 2444.257 kb on + strand, within RR42_RS11420 at 2444.257 kb on + strand, within RR42_RS11420 at 2444.257 kb on + strand, within RR42_RS11420 at 2444.257 kb on + strand, within RR42_RS11420 at 2444.257 kb on + strand, within RR42_RS11420 at 2444.258 kb on - strand, within RR42_RS11420 at 2444.258 kb on - strand, within RR42_RS11420 at 2444.258 kb on - strand, within RR42_RS11420 at 2444.336 kb on + strand, within RR42_RS11420 at 2444.336 kb on + strand, within RR42_RS11420 at 2444.337 kb on - strand, within RR42_RS11420 at 2444.337 kb on - strand, within RR42_RS11420 at 2444.401 kb on + strand, within RR42_RS11420 at 2444.419 kb on + strand, within RR42_RS11420 at 2444.438 kb on + strand, within RR42_RS11420 at 2444.438 kb on + strand, within RR42_RS11420 at 2444.438 kb on + strand, within RR42_RS11420 at 2444.438 kb on + strand, within RR42_RS11420 at 2444.439 kb on - strand, within RR42_RS11420 at 2444.452 kb on + strand, within RR42_RS11420 at 2444.452 kb on + strand, within RR42_RS11420 at 2444.452 kb on + strand, within RR42_RS11420 at 2444.452 kb on + strand, within RR42_RS11420 at 2444.453 kb on - strand, within RR42_RS11420 at 2444.453 kb on - strand, within RR42_RS11420 at 2444.453 kb on - strand, within RR42_RS11420 at 2444.502 kb on - strand, within RR42_RS11420 at 2444.564 kb on - strand, within RR42_RS11420 at 2444.578 kb on - strand, within RR42_RS11420 at 2444.623 kb on - strand, within RR42_RS11420 at 2444.623 kb on - strand, within RR42_RS11420 at 2444.624 kb on - strand, within RR42_RS11420
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 2,442,617 - -0.7 2,442,623 + +0.5 2,442,623 + -1.7 2,442,624 - -0.1 2,442,624 - +0.2 2,442,624 - -1.1 2,442,624 - +1.8 2,442,803 + -1.9 2,442,803 + -1.7 2,442,804 - -2.8 2,442,808 + +0.6 2,442,809 - +0.0 2,443,206 - RR42_RS11410 0.89 -0.8 2,443,326 + +0.7 2,443,458 + RR42_RS11415 0.17 -1.8 2,443,458 + RR42_RS11415 0.17 -1.0 2,443,459 - RR42_RS11415 0.17 -0.6 2,443,459 - RR42_RS11415 0.17 -1.5 2,443,459 - RR42_RS11415 0.17 -1.4 2,443,565 - RR42_RS11415 0.62 -0.9 2,443,656 + -0.4 2,443,657 - -0.2 2,443,657 - -0.1 2,443,680 + -2.4 2,443,681 - -1.0 2,443,804 - -0.3 2,443,804 - +0.5 2,443,804 - +0.9 2,443,904 + -0.4 2,443,936 + -0.6 2,443,936 + -0.8 2,443,963 + -0.4 2,444,062 - RR42_RS11420 0.13 -0.3 2,444,062 - RR42_RS11420 0.13 -0.4 2,444,194 - RR42_RS11420 0.26 -0.4 2,444,257 + RR42_RS11420 0.32 +0.4 2,444,257 + RR42_RS11420 0.32 -1.0 2,444,257 + RR42_RS11420 0.32 -0.3 2,444,257 + RR42_RS11420 0.32 +0.5 2,444,257 + RR42_RS11420 0.32 +0.5 2,444,258 - RR42_RS11420 0.32 -0.1 2,444,258 - RR42_RS11420 0.32 +0.2 2,444,258 - RR42_RS11420 0.32 +0.2 2,444,336 + RR42_RS11420 0.40 +0.2 2,444,336 + RR42_RS11420 0.40 +0.1 2,444,337 - RR42_RS11420 0.40 +0.2 2,444,337 - RR42_RS11420 0.40 -0.3 2,444,401 + RR42_RS11420 0.46 -0.7 2,444,419 + RR42_RS11420 0.48 +0.4 2,444,438 + RR42_RS11420 0.50 -0.8 2,444,438 + RR42_RS11420 0.50 +0.4 2,444,438 + RR42_RS11420 0.50 -1.8 2,444,438 + RR42_RS11420 0.50 +0.5 2,444,439 - RR42_RS11420 0.50 -0.2 2,444,452 + RR42_RS11420 0.51 +0.0 2,444,452 + RR42_RS11420 0.51 -0.1 2,444,452 + RR42_RS11420 0.51 -1.4 2,444,452 + RR42_RS11420 0.51 -0.2 2,444,453 - RR42_RS11420 0.51 +0.8 2,444,453 - RR42_RS11420 0.51 -1.3 2,444,453 - RR42_RS11420 0.51 -0.9 2,444,502 - RR42_RS11420 0.56 +1.2 2,444,564 - RR42_RS11420 0.62 -3.6 2,444,578 - RR42_RS11420 0.64 +1.3 2,444,623 - RR42_RS11420 0.68 +0.4 2,444,623 - RR42_RS11420 0.68 +0.6 2,444,624 - RR42_RS11420 0.68 +0.2
Or see this region's nucleotide sequence