Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS10620

Experiment: R2A with Cobalt chloride 0.3 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS10615 and RR42_RS10620 are separated by 9 nucleotidesRR42_RS10620 and RR42_RS10625 are separated by 42 nucleotides RR42_RS10615: RR42_RS10615 - 2-hydroxyacid dehydrogenase, at 2,267,898 to 2,268,923 _RS10615 RR42_RS10620: RR42_RS10620 - amidohydrolase, at 2,268,933 to 2,269,763 _RS10620 RR42_RS10625: RR42_RS10625 - glucarate dehydratase, at 2,269,806 to 2,271,164 _RS10625 Position (kb) 2268 2269 2270Strain fitness (log2 ratio) -2 -1 0 1at 2268.381 kb on + strand, within RR42_RS10615at 2268.382 kb on - strand, within RR42_RS10615at 2268.673 kb on + strand, within RR42_RS10615at 2268.673 kb on + strand, within RR42_RS10615at 2268.673 kb on + strand, within RR42_RS10615at 2268.674 kb on - strand, within RR42_RS10615at 2268.921 kb on + strandat 2269.003 kb on - strandat 2269.255 kb on - strand, within RR42_RS10620at 2269.255 kb on - strand, within RR42_RS10620at 2269.348 kb on + strand, within RR42_RS10620at 2269.521 kb on + strand, within RR42_RS10620at 2269.594 kb on - strand, within RR42_RS10620at 2269.717 kb on + strandat 2269.717 kb on + strandat 2269.727 kb on + strandat 2269.743 kb on + strandat 2269.743 kb on + strandat 2269.744 kb on - strandat 2269.826 kb on + strandat 2269.826 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM
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2,268,381 + RR42_RS10615 0.47 -0.6
2,268,382 - RR42_RS10615 0.47 +0.3
2,268,673 + RR42_RS10615 0.76 +0.5
2,268,673 + RR42_RS10615 0.76 -0.3
2,268,673 + RR42_RS10615 0.76 -0.1
2,268,674 - RR42_RS10615 0.76 -2.5
2,268,921 + -0.6
2,269,003 - -2.2
2,269,255 - RR42_RS10620 0.39 -0.1
2,269,255 - RR42_RS10620 0.39 +0.1
2,269,348 + RR42_RS10620 0.50 +0.8
2,269,521 + RR42_RS10620 0.71 +0.4
2,269,594 - RR42_RS10620 0.80 -0.0
2,269,717 + -2.0
2,269,717 + +1.2
2,269,727 + -0.8
2,269,743 + -0.1
2,269,743 + -0.1
2,269,744 - +0.6
2,269,826 + +0.9
2,269,826 + +0.1

Or see this region's nucleotide sequence