Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10505 and RR42_RS10510 are separated by 107 nucleotides RR42_RS10510 and RR42_RS10515 are separated by 31 nucleotides RR42_RS10515 and RR42_RS10520 are separated by 47 nucleotides
RR42_RS10505: RR42_RS10505 - citrate synthase, at 2,245,855 to 2,246,997
_RS10505
RR42_RS10510: RR42_RS10510 - dihydrofolate reductase, at 2,247,105 to 2,248,058
_RS10510
RR42_RS10515: RR42_RS10515 - fumarylacetoacetate hydrolase, at 2,248,090 to 2,248,770
_RS10515
RR42_RS10520: RR42_RS10520 - 3-oxoacyl-ACP reductase, at 2,248,818 to 2,249,576
_RS10520
Position (kb)
2247
2248
2249 Strain fitness (log2 ratio)
-2
-1
0
1 at 2246.144 kb on + strand, within RR42_RS10505 at 2246.144 kb on + strand, within RR42_RS10505 at 2246.144 kb on + strand, within RR42_RS10505 at 2246.729 kb on + strand, within RR42_RS10505 at 2246.730 kb on - strand, within RR42_RS10505 at 2246.923 kb on + strand at 2246.923 kb on + strand at 2246.924 kb on - strand at 2246.924 kb on - strand at 2246.927 kb on + strand at 2246.927 kb on + strand at 2246.927 kb on + strand at 2246.927 kb on + strand at 2246.927 kb on + strand at 2246.927 kb on + strand at 2246.927 kb on + strand at 2246.927 kb on + strand at 2246.928 kb on - strand at 2246.928 kb on - strand at 2246.928 kb on - strand at 2246.933 kb on + strand at 2247.067 kb on + strand at 2247.068 kb on - strand at 2247.174 kb on + strand at 2247.175 kb on - strand at 2247.175 kb on - strand at 2247.190 kb on - strand at 2247.266 kb on + strand, within RR42_RS10510 at 2247.358 kb on - strand, within RR42_RS10510 at 2247.358 kb on - strand, within RR42_RS10510 at 2247.631 kb on - strand, within RR42_RS10510 at 2247.632 kb on + strand, within RR42_RS10510 at 2247.633 kb on - strand, within RR42_RS10510 at 2248.071 kb on - strand at 2248.192 kb on + strand, within RR42_RS10515 at 2248.373 kb on - strand, within RR42_RS10515 at 2248.396 kb on + strand, within RR42_RS10515 at 2248.511 kb on + strand, within RR42_RS10515 at 2248.751 kb on + strand at 2248.823 kb on + strand at 2248.823 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 2,246,144 + RR42_RS10505 0.25 -0.9 2,246,144 + RR42_RS10505 0.25 -0.9 2,246,144 + RR42_RS10505 0.25 +0.6 2,246,729 + RR42_RS10505 0.76 -1.4 2,246,730 - RR42_RS10505 0.77 +0.6 2,246,923 + -0.5 2,246,923 + +0.0 2,246,924 - -0.3 2,246,924 - -1.6 2,246,927 + -0.4 2,246,927 + -1.0 2,246,927 + -0.3 2,246,927 + +0.4 2,246,927 + +0.7 2,246,927 + +0.2 2,246,927 + +0.3 2,246,927 + +0.3 2,246,928 - -0.5 2,246,928 - -0.4 2,246,928 - +0.0 2,246,933 + -1.1 2,247,067 + +0.8 2,247,068 - +0.2 2,247,174 + +0.5 2,247,175 - -0.7 2,247,175 - -1.9 2,247,190 - -0.2 2,247,266 + RR42_RS10510 0.17 +1.7 2,247,358 - RR42_RS10510 0.27 +0.5 2,247,358 - RR42_RS10510 0.27 -1.4 2,247,631 - RR42_RS10510 0.55 -2.5 2,247,632 + RR42_RS10510 0.55 -0.3 2,247,633 - RR42_RS10510 0.55 -0.6 2,248,071 - -0.8 2,248,192 + RR42_RS10515 0.15 -0.8 2,248,373 - RR42_RS10515 0.42 -0.3 2,248,396 + RR42_RS10515 0.45 +0.8 2,248,511 + RR42_RS10515 0.62 -0.2 2,248,751 + -0.4 2,248,823 + +0.2 2,248,823 + -0.9
Or see this region's nucleotide sequence