Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS09305

Experiment: R2A with Cobalt chloride 0.3 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS09300 and RR42_RS09305 are separated by 153 nucleotidesRR42_RS09305 and RR42_RS09310 are separated by 18 nucleotides RR42_RS09300: RR42_RS09300 - aromatic ring-opening dioxygenase LigB, at 1,966,603 to 1,967,433 _RS09300 RR42_RS09305: RR42_RS09305 - LysR family transcriptional regulator, at 1,967,587 to 1,968,483 _RS09305 RR42_RS09310: RR42_RS09310 - IclR family transcriptional regulator, at 1,968,502 to 1,969,440 _RS09310 Position (kb) 1967 1968 1969Strain fitness (log2 ratio) -3 -2 -1 0 1at 1966.652 kb on + strandat 1966.652 kb on + strandat 1966.652 kb on + strandat 1966.652 kb on + strandat 1966.652 kb on + strandat 1966.652 kb on + strandat 1966.653 kb on - strandat 1966.653 kb on - strandat 1966.653 kb on - strandat 1966.677 kb on - strandat 1966.677 kb on - strandat 1966.803 kb on - strand, within RR42_RS09300at 1967.306 kb on - strand, within RR42_RS09300at 1967.495 kb on - strandat 1967.495 kb on - strandat 1967.500 kb on - strandat 1967.500 kb on - strandat 1967.541 kb on + strandat 1967.543 kb on + strandat 1967.939 kb on - strand, within RR42_RS09305at 1968.002 kb on - strand, within RR42_RS09305at 1968.080 kb on + strand, within RR42_RS09305at 1968.080 kb on + strand, within RR42_RS09305at 1968.080 kb on + strand, within RR42_RS09305at 1968.081 kb on - strand, within RR42_RS09305at 1968.242 kb on - strand, within RR42_RS09305at 1968.388 kb on + strand, within RR42_RS09305at 1968.398 kb on - strandat 1968.753 kb on - strand, within RR42_RS09310at 1969.092 kb on - strand, within RR42_RS09310at 1969.479 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM
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1,966,652 + +0.4
1,966,652 + +0.2
1,966,652 + -1.1
1,966,652 + -0.2
1,966,652 + +0.6
1,966,652 + +0.1
1,966,653 - -0.0
1,966,653 - -3.2
1,966,653 - +0.6
1,966,677 - -0.8
1,966,677 - -0.4
1,966,803 - RR42_RS09300 0.24 +0.1
1,967,306 - RR42_RS09300 0.85 -1.1
1,967,495 - -1.4
1,967,495 - +0.6
1,967,500 - -0.5
1,967,500 - +0.3
1,967,541 + -0.6
1,967,543 + +0.1
1,967,939 - RR42_RS09305 0.39 -0.7
1,968,002 - RR42_RS09305 0.46 -0.8
1,968,080 + RR42_RS09305 0.55 -1.3
1,968,080 + RR42_RS09305 0.55 -0.5
1,968,080 + RR42_RS09305 0.55 -1.4
1,968,081 - RR42_RS09305 0.55 -0.5
1,968,242 - RR42_RS09305 0.73 -0.6
1,968,388 + RR42_RS09305 0.89 +0.2
1,968,398 - -0.8
1,968,753 - RR42_RS09310 0.27 -0.2
1,969,092 - RR42_RS09310 0.63 -0.1
1,969,479 - +0.0

Or see this region's nucleotide sequence