Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS09045

Experiment: R2A with Cobalt chloride 0.3 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS09030 and RR42_RS09035 are separated by 296 nucleotidesRR42_RS09035 and RR42_RS09040 are separated by 55 nucleotidesRR42_RS09040 and RR42_RS09045 are separated by 248 nucleotidesRR42_RS09045 and RR42_RS09050 are separated by 72 nucleotidesRR42_RS09050 and RR42_RS09055 overlap by 14 nucleotides RR42_RS09030: RR42_RS09030 - DNA-binding protein, at 1,898,036 to 1,900,447 _RS09030 RR42_RS09035: RR42_RS09035 - tRNA-Val, at 1,900,744 to 1,900,819 _RS09035 RR42_RS09040: RR42_RS09040 - tRNA-Asp, at 1,900,875 to 1,900,951 _RS09040 RR42_RS09045: RR42_RS09045 - peptidylprolyl isomerase, at 1,901,200 to 1,903,149 _RS09045 RR42_RS09050: RR42_RS09050 - arylesterase, at 1,903,222 to 1,903,917 _RS09050 RR42_RS09055: RR42_RS09055 - ABC transporter, at 1,903,904 to 1,904,602 _RS09055 Position (kb) 1901 1902 1903 1904Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1900.360 kb on + strandat 1900.470 kb on + strandat 1900.470 kb on + strandat 1900.471 kb on - strandat 1900.607 kb on + strandat 1900.607 kb on + strandat 1900.607 kb on + strandat 1900.626 kb on + strandat 1900.627 kb on - strandat 1900.653 kb on + strandat 1900.696 kb on + strandat 1900.896 kb on - strand, within RR42_RS09040at 1901.092 kb on - strandat 1901.093 kb on + strandat 1901.093 kb on + strandat 1901.094 kb on - strandat 1901.094 kb on - strandat 1901.094 kb on - strandat 1901.152 kb on - strandat 1901.152 kb on - strandat 1901.152 kb on - strandat 1901.293 kb on + strandat 1901.293 kb on + strandat 1901.346 kb on + strandat 1901.419 kb on + strand, within RR42_RS09045at 1901.617 kb on + strand, within RR42_RS09045at 1901.617 kb on + strand, within RR42_RS09045at 1901.617 kb on + strand, within RR42_RS09045at 1901.618 kb on - strand, within RR42_RS09045at 1901.854 kb on + strand, within RR42_RS09045at 1901.854 kb on + strand, within RR42_RS09045at 1901.855 kb on - strand, within RR42_RS09045at 1901.855 kb on - strand, within RR42_RS09045at 1901.857 kb on + strand, within RR42_RS09045at 1901.908 kb on + strand, within RR42_RS09045at 1902.245 kb on - strand, within RR42_RS09045at 1902.289 kb on + strand, within RR42_RS09045at 1902.403 kb on + strand, within RR42_RS09045at 1902.432 kb on + strand, within RR42_RS09045at 1902.433 kb on - strand, within RR42_RS09045at 1902.439 kb on + strand, within RR42_RS09045at 1902.440 kb on - strand, within RR42_RS09045at 1902.440 kb on - strand, within RR42_RS09045at 1902.440 kb on - strand, within RR42_RS09045at 1902.724 kb on + strand, within RR42_RS09045at 1902.724 kb on + strand, within RR42_RS09045at 1902.725 kb on - strand, within RR42_RS09045at 1902.725 kb on - strand, within RR42_RS09045at 1902.726 kb on + strand, within RR42_RS09045at 1902.727 kb on - strand, within RR42_RS09045at 1902.727 kb on - strand, within RR42_RS09045at 1902.910 kb on + strand, within RR42_RS09045at 1902.910 kb on + strand, within RR42_RS09045at 1902.910 kb on + strand, within RR42_RS09045at 1902.910 kb on + strand, within RR42_RS09045at 1902.910 kb on + strand, within RR42_RS09045at 1902.911 kb on - strand, within RR42_RS09045at 1902.940 kb on + strand, within RR42_RS09045at 1902.949 kb on + strand, within RR42_RS09045at 1902.950 kb on - strand, within RR42_RS09045at 1903.058 kb on - strandat 1903.058 kb on - strandat 1903.058 kb on - strandat 1903.058 kb on - strandat 1903.058 kb on - strandat 1903.058 kb on - strandat 1903.223 kb on + strandat 1903.223 kb on + strandat 1903.224 kb on - strandat 1903.224 kb on - strandat 1903.231 kb on - strandat 1903.416 kb on - strand, within RR42_RS09050at 1903.452 kb on - strand, within RR42_RS09050at 1903.683 kb on + strand, within RR42_RS09050at 1903.683 kb on + strand, within RR42_RS09050at 1903.684 kb on - strand, within RR42_RS09050at 1903.684 kb on - strand, within RR42_RS09050

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM
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1,900,360 + +0.5
1,900,470 + +0.6
1,900,470 + +0.1
1,900,471 - +0.4
1,900,607 + -0.3
1,900,607 + +0.1
1,900,607 + -1.0
1,900,626 + +0.1
1,900,627 - +0.9
1,900,653 + +2.2
1,900,696 + +1.5
1,900,896 - RR42_RS09040 0.27 +0.2
1,901,092 - +0.6
1,901,093 + -0.0
1,901,093 + +0.3
1,901,094 - +1.4
1,901,094 - +1.0
1,901,094 - +0.4
1,901,152 - +2.4
1,901,152 - +1.1
1,901,152 - +2.3
1,901,293 + +1.0
1,901,293 + +2.0
1,901,346 + +1.9
1,901,419 + RR42_RS09045 0.11 +1.3
1,901,617 + RR42_RS09045 0.21 +1.9
1,901,617 + RR42_RS09045 0.21 +1.9
1,901,617 + RR42_RS09045 0.21 +1.9
1,901,618 - RR42_RS09045 0.21 +1.5
1,901,854 + RR42_RS09045 0.34 +0.6
1,901,854 + RR42_RS09045 0.34 +2.7
1,901,855 - RR42_RS09045 0.34 +2.1
1,901,855 - RR42_RS09045 0.34 +1.5
1,901,857 + RR42_RS09045 0.34 +1.1
1,901,908 + RR42_RS09045 0.36 +2.2
1,902,245 - RR42_RS09045 0.54 +0.6
1,902,289 + RR42_RS09045 0.56 +1.6
1,902,403 + RR42_RS09045 0.62 +2.6
1,902,432 + RR42_RS09045 0.63 +1.1
1,902,433 - RR42_RS09045 0.63 +1.3
1,902,439 + RR42_RS09045 0.64 +3.5
1,902,440 - RR42_RS09045 0.64 +1.3
1,902,440 - RR42_RS09045 0.64 +1.2
1,902,440 - RR42_RS09045 0.64 +2.3
1,902,724 + RR42_RS09045 0.78 +1.4
1,902,724 + RR42_RS09045 0.78 +1.6
1,902,725 - RR42_RS09045 0.78 +1.7
1,902,725 - RR42_RS09045 0.78 +0.4
1,902,726 + RR42_RS09045 0.78 +1.3
1,902,727 - RR42_RS09045 0.78 +1.6
1,902,727 - RR42_RS09045 0.78 -0.4
1,902,910 + RR42_RS09045 0.88 +2.5
1,902,910 + RR42_RS09045 0.88 +1.4
1,902,910 + RR42_RS09045 0.88 +1.9
1,902,910 + RR42_RS09045 0.88 +2.3
1,902,910 + RR42_RS09045 0.88 -0.8
1,902,911 - RR42_RS09045 0.88 +1.7
1,902,940 + RR42_RS09045 0.89 +1.8
1,902,949 + RR42_RS09045 0.90 +2.3
1,902,950 - RR42_RS09045 0.90 +1.6
1,903,058 - +0.3
1,903,058 - +0.7
1,903,058 - +1.5
1,903,058 - +0.7
1,903,058 - +1.9
1,903,058 - +1.4
1,903,223 + +0.2
1,903,223 + -0.7
1,903,224 - +2.4
1,903,224 - +0.3
1,903,231 - +0.4
1,903,416 - RR42_RS09050 0.28 +1.0
1,903,452 - RR42_RS09050 0.33 +0.3
1,903,683 + RR42_RS09050 0.66 -1.8
1,903,683 + RR42_RS09050 0.66 +0.8
1,903,684 - RR42_RS09050 0.66 -0.3
1,903,684 - RR42_RS09050 0.66 +1.9

Or see this region's nucleotide sequence