Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08585 and RR42_RS08590 are separated by 14 nucleotides RR42_RS08590 and RR42_RS08595 are separated by 281 nucleotides RR42_RS08595 and RR42_RS08600 overlap by 4 nucleotides RR42_RS08600 and RR42_RS08605 overlap by 4 nucleotides RR42_RS08605 and RR42_RS08610 overlap by 4 nucleotides
RR42_RS08585: RR42_RS08585 - baseplate assembly protein, at 1,804,363 to 1,804,707
_RS08585
RR42_RS08590: RR42_RS08590 - baseplate assembly protein, at 1,804,722 to 1,805,339
_RS08590
RR42_RS08595: RR42_RS08595 - virion morphogenesis protein, at 1,805,621 to 1,806,067
_RS08595
RR42_RS08600: RR42_RS08600 - tail protein, at 1,806,064 to 1,806,504
_RS08600
RR42_RS08605: RR42_RS08605 - hypothetical protein, at 1,806,501 to 1,807,010
_RS08605
RR42_RS08610: RR42_RS08610 - peptidoglycan-binding protein, at 1,807,007 to 1,807,813
_RS08610
Position (kb) 1805
1806
1807 Strain fitness (log2 ratio) -2
-1
0
1 at 1804.804 kb on - strand, within RR42_RS08590 at 1804.962 kb on + strand, within RR42_RS08590 at 1805.412 kb on + strand at 1805.412 kb on + strand at 1805.493 kb on + strand at 1805.494 kb on - strand at 1805.594 kb on + strand at 1805.611 kb on + strand at 1806.565 kb on - strand, within RR42_RS08605 at 1806.595 kb on + strand, within RR42_RS08605 at 1806.595 kb on + strand, within RR42_RS08605 at 1806.596 kb on - strand, within RR42_RS08605 at 1806.596 kb on - strand, within RR42_RS08605 at 1806.732 kb on - strand, within RR42_RS08605 at 1806.732 kb on - strand, within RR42_RS08605 at 1806.732 kb on - strand, within RR42_RS08605 at 1806.873 kb on + strand, within RR42_RS08605 at 1806.926 kb on - strand, within RR42_RS08605 at 1807.011 kb on - strand at 1807.044 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 1,804,804 - RR42_RS08590 0.13 -0.1 1,804,962 + RR42_RS08590 0.39 +0.1 1,805,412 + -0.2 1,805,412 + -1.4 1,805,493 + -0.7 1,805,494 - -0.3 1,805,594 + -0.4 1,805,611 + +0.0 1,806,565 - RR42_RS08605 0.13 +0.7 1,806,595 + RR42_RS08605 0.18 -2.0 1,806,595 + RR42_RS08605 0.18 +0.1 1,806,596 - RR42_RS08605 0.19 +0.4 1,806,596 - RR42_RS08605 0.19 -1.7 1,806,732 - RR42_RS08605 0.45 -0.3 1,806,732 - RR42_RS08605 0.45 +0.9 1,806,732 - RR42_RS08605 0.45 +1.6 1,806,873 + RR42_RS08605 0.73 +0.5 1,806,926 - RR42_RS08605 0.83 +0.2 1,807,011 - -2.1 1,807,044 + +0.4
Or see this region's nucleotide sequence