Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06560 and RR42_RS06565 are separated by 95 nucleotides RR42_RS06565 and RR42_RS06570 are separated by 14 nucleotides RR42_RS06570 and RR42_RS06575 are separated by 202 nucleotides
RR42_RS06560: RR42_RS06560 - succinyl-CoA:3-ketoacid-CoA transferase, at 1,434,556 to 1,435,194
_RS06560
RR42_RS06565: RR42_RS06565 - metal-binding protein, at 1,435,290 to 1,435,784
_RS06565
RR42_RS06570: RR42_RS06570 - 3-hydroxybutyrate dehydrogenase, at 1,435,799 to 1,436,575
_RS06570
RR42_RS06575: RR42_RS06575 - alpha/beta hydrolase, at 1,436,778 to 1,437,665
_RS06575
Position (kb)
1435
1436
1437 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1435.309 kb on + strand at 1435.309 kb on + strand at 1435.310 kb on - strand at 1435.310 kb on - strand at 1435.658 kb on - strand, within RR42_RS06565 at 1435.658 kb on - strand, within RR42_RS06565 at 1435.967 kb on - strand, within RR42_RS06570 at 1436.119 kb on - strand, within RR42_RS06570 at 1436.119 kb on - strand, within RR42_RS06570 at 1436.119 kb on - strand, within RR42_RS06570 at 1436.119 kb on - strand, within RR42_RS06570 at 1436.570 kb on - strand at 1436.686 kb on + strand at 1436.687 kb on - strand at 1436.687 kb on - strand at 1436.701 kb on - strand at 1436.760 kb on + strand at 1436.760 kb on + strand at 1436.848 kb on - strand at 1436.848 kb on - strand at 1436.956 kb on - strand, within RR42_RS06575 at 1436.956 kb on - strand, within RR42_RS06575 at 1436.978 kb on - strand, within RR42_RS06575 at 1436.978 kb on - strand, within RR42_RS06575 at 1437.022 kb on - strand, within RR42_RS06575 at 1437.036 kb on + strand at 1437.037 kb on - strand, within RR42_RS06575 at 1437.037 kb on - strand, within RR42_RS06575 at 1437.037 kb on - strand, within RR42_RS06575 at 1437.037 kb on - strand, within RR42_RS06575 at 1437.037 kb on - strand, within RR42_RS06575 at 1437.222 kb on + strand, within RR42_RS06575 at 1437.222 kb on + strand, within RR42_RS06575 at 1437.223 kb on - strand, within RR42_RS06575 at 1437.223 kb on - strand, within RR42_RS06575 at 1437.388 kb on - strand, within RR42_RS06575 at 1437.499 kb on + strand, within RR42_RS06575
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 1,435,309 + +0.3 1,435,309 + +0.1 1,435,310 - -1.1 1,435,310 - -0.2 1,435,658 - RR42_RS06565 0.74 +0.3 1,435,658 - RR42_RS06565 0.74 +0.2 1,435,967 - RR42_RS06570 0.22 -1.5 1,436,119 - RR42_RS06570 0.41 +0.3 1,436,119 - RR42_RS06570 0.41 +0.2 1,436,119 - RR42_RS06570 0.41 +0.3 1,436,119 - RR42_RS06570 0.41 +0.9 1,436,570 - -0.8 1,436,686 + +0.3 1,436,687 - -2.6 1,436,687 - -1.7 1,436,701 - -0.4 1,436,760 + +1.1 1,436,760 + +0.9 1,436,848 - -4.7 1,436,848 - -0.8 1,436,956 - RR42_RS06575 0.20 -1.8 1,436,956 - RR42_RS06575 0.20 -1.4 1,436,978 - RR42_RS06575 0.23 -2.9 1,436,978 - RR42_RS06575 0.23 -2.0 1,437,022 - RR42_RS06575 0.27 -1.9 1,437,036 + -0.2 1,437,037 - RR42_RS06575 0.29 -1.2 1,437,037 - RR42_RS06575 0.29 -2.4 1,437,037 - RR42_RS06575 0.29 -2.0 1,437,037 - RR42_RS06575 0.29 -2.9 1,437,037 - RR42_RS06575 0.29 -0.8 1,437,222 + RR42_RS06575 0.50 -0.9 1,437,222 + RR42_RS06575 0.50 +0.8 1,437,223 - RR42_RS06575 0.50 -2.7 1,437,223 - RR42_RS06575 0.50 -1.4 1,437,388 - RR42_RS06575 0.69 +0.2 1,437,499 + RR42_RS06575 0.81 +1.6
Or see this region's nucleotide sequence