Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04445 and RR42_RS04450 are separated by 81 nucleotides RR42_RS04450 and RR42_RS04455 are separated by 122 nucleotides RR42_RS04455 and RR42_RS04460 are separated by 117 nucleotides
RR42_RS04445: RR42_RS04445 - DNA gyrase subunit A, at 992,301 to 995,015
_RS04445
RR42_RS04450: RR42_RS04450 - hypothetical protein, at 995,097 to 995,660
_RS04450
RR42_RS04455: RR42_RS04455 - MFS transporter, at 995,783 to 996,943
_RS04455
RR42_RS04460: RR42_RS04460 - chorismate mutase, at 997,061 to 998,200
_RS04460
Position (kb) 995
996
997 Strain fitness (log2 ratio) -2
-1
0
1
2 at 995.082 kb on + strand at 995.082 kb on + strand at 995.082 kb on + strand at 995.658 kb on + strand at 995.746 kb on + strand at 995.746 kb on + strand at 997.057 kb on + strand at 997.057 kb on + strand at 997.057 kb on + strand at 997.058 kb on - strand at 997.058 kb on - strand at 997.247 kb on + strand, within RR42_RS04460 at 997.247 kb on + strand, within RR42_RS04460 at 997.247 kb on + strand, within RR42_RS04460 at 997.326 kb on + strand, within RR42_RS04460 at 997.443 kb on - strand, within RR42_RS04460 at 997.739 kb on + strand, within RR42_RS04460 at 997.739 kb on + strand, within RR42_RS04460
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 995,082 + -0.6 995,082 + +0.3 995,082 + +0.1 995,658 + -1.2 995,746 + -2.3 995,746 + -1.9 997,057 + -1.2 997,057 + -2.4 997,057 + +0.2 997,058 - +1.8 997,058 - -0.2 997,247 + RR42_RS04460 0.16 +0.1 997,247 + RR42_RS04460 0.16 +1.7 997,247 + RR42_RS04460 0.16 +0.5 997,326 + RR42_RS04460 0.23 +0.2 997,443 - RR42_RS04460 0.34 +0.3 997,739 + RR42_RS04460 0.59 +0.4 997,739 + RR42_RS04460 0.59 +0.7
Or see this region's nucleotide sequence