Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04395 and RR42_RS04400 are separated by 30 nucleotides RR42_RS04400 and RR42_RS04405 are separated by 143 nucleotides RR42_RS04405 and RR42_RS04410 are separated by 20 nucleotides
RR42_RS04395: RR42_RS04395 - cysteine ABC transporter substrate-binding protein, at 978,133 to 978,930
_RS04395
RR42_RS04400: RR42_RS04400 - ABC transporter permease, at 978,961 to 979,620
_RS04400
RR42_RS04405: RR42_RS04405 - cyanophycin synthetase, at 979,764 to 982,334
_RS04405
RR42_RS04410: RR42_RS04410 - cyanophycin synthetase, at 982,355 to 984,991
_RS04410
Position (kb) 979
980
981
982
983 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 979.130 kb on - strand, within RR42_RS04400 at 979.180 kb on + strand, within RR42_RS04400 at 979.180 kb on + strand, within RR42_RS04400 at 979.181 kb on - strand, within RR42_RS04400 at 979.181 kb on - strand, within RR42_RS04400 at 979.184 kb on - strand, within RR42_RS04400 at 979.261 kb on + strand, within RR42_RS04400 at 979.262 kb on - strand, within RR42_RS04400 at 979.325 kb on - strand, within RR42_RS04400 at 979.325 kb on - strand, within RR42_RS04400 at 979.360 kb on + strand, within RR42_RS04400 at 979.361 kb on - strand, within RR42_RS04400 at 979.504 kb on + strand, within RR42_RS04400 at 979.522 kb on + strand, within RR42_RS04400 at 979.523 kb on - strand, within RR42_RS04400 at 979.539 kb on + strand, within RR42_RS04400 at 979.540 kb on - strand, within RR42_RS04400 at 979.540 kb on - strand, within RR42_RS04400 at 979.543 kb on + strand, within RR42_RS04400 at 979.543 kb on + strand, within RR42_RS04400 at 979.543 kb on + strand, within RR42_RS04400 at 979.572 kb on + strand at 979.632 kb on + strand at 979.632 kb on + strand at 979.633 kb on - strand at 979.633 kb on - strand at 979.633 kb on - strand at 979.633 kb on - strand at 979.633 kb on - strand at 979.644 kb on + strand at 979.644 kb on + strand at 979.790 kb on + strand at 979.866 kb on - strand at 979.991 kb on - strand at 979.991 kb on - strand at 980.180 kb on - strand, within RR42_RS04405 at 980.188 kb on - strand, within RR42_RS04405 at 980.188 kb on - strand, within RR42_RS04405 at 980.442 kb on - strand, within RR42_RS04405 at 980.812 kb on + strand, within RR42_RS04405 at 980.812 kb on + strand, within RR42_RS04405 at 981.483 kb on - strand, within RR42_RS04405 at 981.485 kb on - strand, within RR42_RS04405 at 981.530 kb on - strand, within RR42_RS04405 at 981.838 kb on + strand, within RR42_RS04405 at 982.024 kb on + strand, within RR42_RS04405 at 982.024 kb on + strand, within RR42_RS04405 at 982.032 kb on + strand, within RR42_RS04405 at 982.032 kb on + strand, within RR42_RS04405 at 982.042 kb on + strand, within RR42_RS04405 at 982.042 kb on + strand, within RR42_RS04405 at 982.042 kb on + strand, within RR42_RS04405 at 982.043 kb on - strand, within RR42_RS04405 at 982.457 kb on + strand at 982.457 kb on + strand at 982.458 kb on - strand at 982.458 kb on - strand at 982.458 kb on - strand at 982.533 kb on + strand at 982.534 kb on - strand at 982.534 kb on - strand at 982.945 kb on - strand, within RR42_RS04410 at 983.025 kb on + strand, within RR42_RS04410 at 983.026 kb on - strand, within RR42_RS04410 at 983.026 kb on - strand, within RR42_RS04410 at 983.205 kb on + strand, within RR42_RS04410 at 983.205 kb on + strand, within RR42_RS04410 at 983.206 kb on - strand, within RR42_RS04410 at 983.206 kb on - strand, within RR42_RS04410 at 983.206 kb on - strand, within RR42_RS04410 at 983.229 kb on + strand, within RR42_RS04410 at 983.229 kb on + strand, within RR42_RS04410 at 983.229 kb on + strand, within RR42_RS04410 at 983.229 kb on + strand, within RR42_RS04410 at 983.229 kb on + strand, within RR42_RS04410 at 983.229 kb on + strand, within RR42_RS04410 at 983.229 kb on + strand, within RR42_RS04410 at 983.230 kb on - strand, within RR42_RS04410 at 983.230 kb on - strand, within RR42_RS04410 at 983.232 kb on + strand, within RR42_RS04410 at 983.292 kb on + strand, within RR42_RS04410
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 979,130 - RR42_RS04400 0.26 -0.1 979,180 + RR42_RS04400 0.33 -0.1 979,180 + RR42_RS04400 0.33 +0.2 979,181 - RR42_RS04400 0.33 +0.1 979,181 - RR42_RS04400 0.33 -1.1 979,184 - RR42_RS04400 0.34 -2.6 979,261 + RR42_RS04400 0.45 +0.6 979,262 - RR42_RS04400 0.46 -2.6 979,325 - RR42_RS04400 0.55 +0.6 979,325 - RR42_RS04400 0.55 +0.9 979,360 + RR42_RS04400 0.60 -0.0 979,361 - RR42_RS04400 0.61 -3.5 979,504 + RR42_RS04400 0.82 -0.0 979,522 + RR42_RS04400 0.85 +0.9 979,523 - RR42_RS04400 0.85 +0.7 979,539 + RR42_RS04400 0.88 +0.4 979,540 - RR42_RS04400 0.88 +0.0 979,540 - RR42_RS04400 0.88 -0.8 979,543 + RR42_RS04400 0.88 +1.2 979,543 + RR42_RS04400 0.88 +0.2 979,543 + RR42_RS04400 0.88 -0.1 979,572 + +0.1 979,632 + +0.7 979,632 + +0.8 979,633 - -1.6 979,633 - +0.4 979,633 - -0.4 979,633 - -0.6 979,633 - -0.5 979,644 + -1.4 979,644 + -0.2 979,790 + +1.8 979,866 - +1.0 979,991 - +2.0 979,991 - +1.5 980,180 - RR42_RS04405 0.16 +0.7 980,188 - RR42_RS04405 0.16 +3.1 980,188 - RR42_RS04405 0.16 +0.6 980,442 - RR42_RS04405 0.26 +1.1 980,812 + RR42_RS04405 0.41 -1.5 980,812 + RR42_RS04405 0.41 +0.9 981,483 - RR42_RS04405 0.67 +0.0 981,485 - RR42_RS04405 0.67 +0.9 981,530 - RR42_RS04405 0.69 +1.8 981,838 + RR42_RS04405 0.81 +0.9 982,024 + RR42_RS04405 0.88 +2.1 982,024 + RR42_RS04405 0.88 +1.4 982,032 + RR42_RS04405 0.88 +0.6 982,032 + RR42_RS04405 0.88 +1.4 982,042 + RR42_RS04405 0.89 +2.5 982,042 + RR42_RS04405 0.89 +0.3 982,042 + RR42_RS04405 0.89 +0.5 982,043 - RR42_RS04405 0.89 +2.3 982,457 + -0.5 982,457 + +1.1 982,458 - -0.5 982,458 - -0.6 982,458 - +1.7 982,533 + +1.9 982,534 - -2.3 982,534 - +1.2 982,945 - RR42_RS04410 0.22 +1.9 983,025 + RR42_RS04410 0.25 -0.0 983,026 - RR42_RS04410 0.25 +2.2 983,026 - RR42_RS04410 0.25 +1.7 983,205 + RR42_RS04410 0.32 +1.2 983,205 + RR42_RS04410 0.32 +2.0 983,206 - RR42_RS04410 0.32 -1.0 983,206 - RR42_RS04410 0.32 +1.9 983,206 - RR42_RS04410 0.32 +3.0 983,229 + RR42_RS04410 0.33 +0.9 983,229 + RR42_RS04410 0.33 +1.1 983,229 + RR42_RS04410 0.33 +2.0 983,229 + RR42_RS04410 0.33 +0.8 983,229 + RR42_RS04410 0.33 +1.7 983,229 + RR42_RS04410 0.33 +1.6 983,229 + RR42_RS04410 0.33 +1.6 983,230 - RR42_RS04410 0.33 +0.3 983,230 - RR42_RS04410 0.33 -0.7 983,232 + RR42_RS04410 0.33 -1.0 983,292 + RR42_RS04410 0.36 +0.9
Or see this region's nucleotide sequence