Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS02035

Experiment: R2A with Cobalt chloride 0.3 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS02025 and RR42_RS02030 overlap by 4 nucleotidesRR42_RS02030 and RR42_RS02035 are separated by 119 nucleotidesRR42_RS02035 and RR42_RS02040 are separated by 140 nucleotides RR42_RS02025: RR42_RS02025 - 4-diphosphocytidyl-2C-methyl-D-erythritol kinase, at 450,651 to 451,529 _RS02025 RR42_RS02030: RR42_RS02030 - membrane protein, at 451,526 to 452,146 _RS02030 RR42_RS02035: RR42_RS02035 - hypothetical protein, at 452,266 to 454,149 _RS02035 RR42_RS02040: RR42_RS02040 - formamidopyrimidine-DNA glycosylase, at 454,290 to 455,171 _RS02040 Position (kb) 452 453 454 455Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 451.820 kb on - strand, within RR42_RS02030at 452.002 kb on - strand, within RR42_RS02030at 452.002 kb on - strand, within RR42_RS02030at 452.267 kb on + strandat 452.267 kb on + strandat 452.296 kb on - strandat 452.296 kb on - strandat 452.298 kb on - strandat 452.339 kb on - strandat 452.339 kb on - strandat 452.523 kb on - strand, within RR42_RS02035at 452.523 kb on - strand, within RR42_RS02035at 452.523 kb on - strand, within RR42_RS02035at 452.523 kb on - strand, within RR42_RS02035at 452.622 kb on - strand, within RR42_RS02035at 452.622 kb on - strand, within RR42_RS02035at 452.622 kb on - strand, within RR42_RS02035at 452.622 kb on - strand, within RR42_RS02035at 452.685 kb on - strand, within RR42_RS02035at 452.867 kb on - strand, within RR42_RS02035at 452.867 kb on - strand, within RR42_RS02035at 452.867 kb on - strand, within RR42_RS02035at 452.869 kb on - strand, within RR42_RS02035at 453.018 kb on - strand, within RR42_RS02035at 453.051 kb on - strand, within RR42_RS02035at 453.105 kb on - strand, within RR42_RS02035at 453.117 kb on - strand, within RR42_RS02035at 453.135 kb on - strand, within RR42_RS02035at 453.255 kb on - strand, within RR42_RS02035at 453.891 kb on - strand, within RR42_RS02035at 454.190 kb on - strandat 454.190 kb on - strandat 454.200 kb on + strandat 454.201 kb on - strandat 454.201 kb on - strandat 454.201 kb on - strandat 454.201 kb on - strandat 454.211 kb on + strandat 454.211 kb on + strandat 454.212 kb on - strandat 454.212 kb on - strandat 454.464 kb on + strand, within RR42_RS02040at 454.464 kb on + strand, within RR42_RS02040at 454.465 kb on - strand, within RR42_RS02040at 454.465 kb on - strand, within RR42_RS02040at 454.792 kb on - strand, within RR42_RS02040at 454.792 kb on - strand, within RR42_RS02040at 454.868 kb on + strand, within RR42_RS02040at 454.868 kb on + strand, within RR42_RS02040at 454.868 kb on + strand, within RR42_RS02040at 454.868 kb on + strand, within RR42_RS02040at 454.868 kb on + strand, within RR42_RS02040at 454.869 kb on - strand, within RR42_RS02040at 454.869 kb on - strand, within RR42_RS02040at 454.869 kb on - strand, within RR42_RS02040at 454.869 kb on - strand, within RR42_RS02040at 454.869 kb on - strand, within RR42_RS02040at 454.869 kb on - strand, within RR42_RS02040at 454.869 kb on - strand, within RR42_RS02040at 454.869 kb on - strand, within RR42_RS02040at 454.872 kb on + strand, within RR42_RS02040at 454.872 kb on + strand, within RR42_RS02040at 454.872 kb on + strand, within RR42_RS02040at 454.872 kb on + strand, within RR42_RS02040at 454.872 kb on + strand, within RR42_RS02040at 454.872 kb on + strand, within RR42_RS02040at 454.872 kb on + strand, within RR42_RS02040at 454.872 kb on + strand, within RR42_RS02040at 454.872 kb on + strand, within RR42_RS02040at 454.872 kb on + strand, within RR42_RS02040at 454.872 kb on + strand, within RR42_RS02040at 454.872 kb on + strand, within RR42_RS02040at 454.873 kb on - strand, within RR42_RS02040at 454.873 kb on - strand, within RR42_RS02040at 454.873 kb on - strand, within RR42_RS02040at 454.873 kb on - strand, within RR42_RS02040at 454.873 kb on - strand, within RR42_RS02040at 454.873 kb on - strand, within RR42_RS02040at 454.873 kb on - strand, within RR42_RS02040at 454.873 kb on - strand, within RR42_RS02040at 454.873 kb on - strand, within RR42_RS02040at 455.071 kb on - strand, within RR42_RS02040at 455.148 kb on + strandat 455.148 kb on + strandat 455.148 kb on + strandat 455.148 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM
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451,820 - RR42_RS02030 0.47 -0.7
452,002 - RR42_RS02030 0.77 +0.1
452,002 - RR42_RS02030 0.77 +0.9
452,267 + -1.0
452,267 + -0.5
452,296 - -0.0
452,296 - +0.0
452,298 - -1.2
452,339 - -2.3
452,339 - -3.3
452,523 - RR42_RS02035 0.14 -0.3
452,523 - RR42_RS02035 0.14 -1.3
452,523 - RR42_RS02035 0.14 +0.6
452,523 - RR42_RS02035 0.14 +0.7
452,622 - RR42_RS02035 0.19 -0.0
452,622 - RR42_RS02035 0.19 -1.2
452,622 - RR42_RS02035 0.19 +0.6
452,622 - RR42_RS02035 0.19 -0.2
452,685 - RR42_RS02035 0.22 +1.9
452,867 - RR42_RS02035 0.32 +0.0
452,867 - RR42_RS02035 0.32 +0.4
452,867 - RR42_RS02035 0.32 +0.9
452,869 - RR42_RS02035 0.32 +0.1
453,018 - RR42_RS02035 0.40 +0.3
453,051 - RR42_RS02035 0.42 +1.1
453,105 - RR42_RS02035 0.45 -1.1
453,117 - RR42_RS02035 0.45 +0.9
453,135 - RR42_RS02035 0.46 -2.4
453,255 - RR42_RS02035 0.52 +0.1
453,891 - RR42_RS02035 0.86 -1.5
454,190 - +0.3
454,190 - +1.0
454,200 + +0.4
454,201 - -1.2
454,201 - -0.1
454,201 - +0.6
454,201 - +0.6
454,211 + +0.7
454,211 + -0.2
454,212 - +0.8
454,212 - -3.1
454,464 + RR42_RS02040 0.20 -1.1
454,464 + RR42_RS02040 0.20 +2.4
454,465 - RR42_RS02040 0.20 -1.5
454,465 - RR42_RS02040 0.20 -0.4
454,792 - RR42_RS02040 0.57 +2.1
454,792 - RR42_RS02040 0.57 -0.3
454,868 + RR42_RS02040 0.66 -0.6
454,868 + RR42_RS02040 0.66 +0.9
454,868 + RR42_RS02040 0.66 -0.9
454,868 + RR42_RS02040 0.66 +2.1
454,868 + RR42_RS02040 0.66 -0.2
454,869 - RR42_RS02040 0.66 -2.1
454,869 - RR42_RS02040 0.66 -0.2
454,869 - RR42_RS02040 0.66 +0.6
454,869 - RR42_RS02040 0.66 -1.6
454,869 - RR42_RS02040 0.66 +0.6
454,869 - RR42_RS02040 0.66 -1.5
454,869 - RR42_RS02040 0.66 +0.5
454,869 - RR42_RS02040 0.66 -0.7
454,872 + RR42_RS02040 0.66 -0.5
454,872 + RR42_RS02040 0.66 +0.3
454,872 + RR42_RS02040 0.66 +0.4
454,872 + RR42_RS02040 0.66 -2.1
454,872 + RR42_RS02040 0.66 +0.3
454,872 + RR42_RS02040 0.66 -0.4
454,872 + RR42_RS02040 0.66 +0.5
454,872 + RR42_RS02040 0.66 -0.1
454,872 + RR42_RS02040 0.66 -1.0
454,872 + RR42_RS02040 0.66 -0.3
454,872 + RR42_RS02040 0.66 -1.2
454,872 + RR42_RS02040 0.66 +0.5
454,873 - RR42_RS02040 0.66 +0.7
454,873 - RR42_RS02040 0.66 +1.2
454,873 - RR42_RS02040 0.66 -1.4
454,873 - RR42_RS02040 0.66 -0.9
454,873 - RR42_RS02040 0.66 -2.1
454,873 - RR42_RS02040 0.66 -0.5
454,873 - RR42_RS02040 0.66 -2.6
454,873 - RR42_RS02040 0.66 -0.4
454,873 - RR42_RS02040 0.66 -0.4
455,071 - RR42_RS02040 0.89 +1.0
455,148 + -0.3
455,148 + -0.8
455,148 + -2.1
455,148 + -0.5

Or see this region's nucleotide sequence