Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01240 and RR42_RS01245 are separated by 52 nucleotides RR42_RS01245 and RR42_RS01250 are separated by 48 nucleotides RR42_RS01250 and RR42_RS01255 are separated by 100 nucleotides RR42_RS01255 and RR42_RS01260 overlap by 4 nucleotides
RR42_RS01240: RR42_RS01240 - membrane protein, at 280,383 to 281,735
_RS01240
RR42_RS01245: RR42_RS01245 - polyphosphate kinase, at 281,788 to 282,597
_RS01245
RR42_RS01250: RR42_RS01250 - diaminopimelate epimerase, at 282,646 to 283,512
_RS01250
RR42_RS01255: RR42_RS01255 - lauroyl acyltransferase, at 283,613 to 284,506
_RS01255
RR42_RS01260: RR42_RS01260 - lauroyl acyltransferase, at 284,503 to 285,441
_RS01260
Position (kb) 282
283
284 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 281.832 kb on - strand at 281.918 kb on + strand, within RR42_RS01245 at 281.918 kb on + strand, within RR42_RS01245 at 281.918 kb on + strand, within RR42_RS01245 at 281.919 kb on - strand, within RR42_RS01245 at 281.919 kb on - strand, within RR42_RS01245 at 281.961 kb on + strand, within RR42_RS01245 at 281.964 kb on - strand, within RR42_RS01245 at 281.964 kb on - strand, within RR42_RS01245 at 282.145 kb on + strand, within RR42_RS01245 at 282.156 kb on - strand, within RR42_RS01245 at 282.284 kb on + strand, within RR42_RS01245 at 282.440 kb on + strand, within RR42_RS01245 at 282.440 kb on + strand, within RR42_RS01245 at 282.642 kb on - strand at 283.523 kb on + strand at 283.523 kb on + strand at 283.617 kb on + strand at 283.617 kb on + strand at 283.669 kb on - strand at 283.672 kb on - strand at 283.785 kb on + strand, within RR42_RS01255 at 283.785 kb on + strand, within RR42_RS01255 at 283.795 kb on - strand, within RR42_RS01255 at 283.795 kb on - strand, within RR42_RS01255 at 283.816 kb on - strand, within RR42_RS01255 at 283.902 kb on + strand, within RR42_RS01255 at 284.075 kb on - strand, within RR42_RS01255 at 284.076 kb on + strand, within RR42_RS01255 at 284.076 kb on + strand, within RR42_RS01255 at 284.077 kb on - strand, within RR42_RS01255 at 284.400 kb on + strand, within RR42_RS01255 at 284.400 kb on + strand, within RR42_RS01255
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 281,832 - -2.4 281,918 + RR42_RS01245 0.16 -0.3 281,918 + RR42_RS01245 0.16 +0.0 281,918 + RR42_RS01245 0.16 -0.5 281,919 - RR42_RS01245 0.16 +1.2 281,919 - RR42_RS01245 0.16 -3.1 281,961 + RR42_RS01245 0.21 +0.4 281,964 - RR42_RS01245 0.22 +0.0 281,964 - RR42_RS01245 0.22 +0.2 282,145 + RR42_RS01245 0.44 +0.0 282,156 - RR42_RS01245 0.45 -0.6 282,284 + RR42_RS01245 0.61 -0.5 282,440 + RR42_RS01245 0.80 -1.1 282,440 + RR42_RS01245 0.80 +0.3 282,642 - -1.9 283,523 + +0.6 283,523 + +0.5 283,617 + -0.1 283,617 + +0.8 283,669 - +0.2 283,672 - +0.2 283,785 + RR42_RS01255 0.19 +0.4 283,785 + RR42_RS01255 0.19 +0.8 283,795 - RR42_RS01255 0.20 +0.5 283,795 - RR42_RS01255 0.20 -0.5 283,816 - RR42_RS01255 0.23 -2.3 283,902 + RR42_RS01255 0.32 -0.3 284,075 - RR42_RS01255 0.52 -0.0 284,076 + RR42_RS01255 0.52 -1.4 284,076 + RR42_RS01255 0.52 -1.0 284,077 - RR42_RS01255 0.52 -1.0 284,400 + RR42_RS01255 0.88 -2.5 284,400 + RR42_RS01255 0.88 +0.5
Or see this region's nucleotide sequence