Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00950 and RR42_RS00955 are separated by 118 nucleotides
RR42_RS00950: RR42_RS00950 - glyoxylate reductase, at 226,613 to 227,554
_RS00950
RR42_RS00955: RR42_RS00955 - hydroxymethylglutaryl-CoA lyase, at 227,673 to 228,608
_RS00955
Position (kb) 226
227
228 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 225.619 kb on - strand at 225.789 kb on + strand at 225.789 kb on + strand at 225.789 kb on + strand at 225.789 kb on + strand at 225.790 kb on - strand at 225.790 kb on - strand at 225.853 kb on - strand at 226.163 kb on + strand at 226.220 kb on + strand at 226.221 kb on - strand at 226.231 kb on + strand at 226.231 kb on + strand at 226.231 kb on + strand at 226.231 kb on + strand at 226.232 kb on - strand at 226.232 kb on - strand at 226.452 kb on + strand at 226.452 kb on + strand at 226.581 kb on + strand at 226.628 kb on + strand at 226.628 kb on + strand at 226.628 kb on + strand at 226.628 kb on + strand at 226.628 kb on + strand at 226.628 kb on + strand at 226.628 kb on + strand at 226.629 kb on - strand at 226.629 kb on - strand at 226.629 kb on - strand at 226.629 kb on - strand at 226.629 kb on - strand at 226.632 kb on + strand at 226.632 kb on + strand at 226.733 kb on + strand, within RR42_RS00950 at 226.734 kb on - strand, within RR42_RS00950 at 226.919 kb on + strand, within RR42_RS00950 at 226.964 kb on + strand, within RR42_RS00950 at 226.965 kb on - strand, within RR42_RS00950 at 226.965 kb on - strand, within RR42_RS00950 at 226.965 kb on - strand, within RR42_RS00950 at 227.276 kb on + strand, within RR42_RS00950 at 227.277 kb on - strand, within RR42_RS00950 at 227.440 kb on + strand, within RR42_RS00950 at 227.441 kb on - strand, within RR42_RS00950 at 227.614 kb on + strand at 227.615 kb on - strand at 227.693 kb on - strand at 227.805 kb on - strand, within RR42_RS00955 at 227.901 kb on + strand, within RR42_RS00955 at 227.921 kb on + strand, within RR42_RS00955 at 227.922 kb on - strand, within RR42_RS00955 at 227.922 kb on - strand, within RR42_RS00955 at 228.158 kb on - strand, within RR42_RS00955 at 228.158 kb on - strand, within RR42_RS00955 at 228.307 kb on - strand, within RR42_RS00955 at 228.307 kb on - strand, within RR42_RS00955 at 228.441 kb on + strand, within RR42_RS00955 at 228.441 kb on + strand, within RR42_RS00955 at 228.442 kb on - strand, within RR42_RS00955 at 228.442 kb on - strand, within RR42_RS00955
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 225,619 - -3.0 225,789 + +0.6 225,789 + -0.6 225,789 + -1.4 225,789 + +0.1 225,790 - +0.2 225,790 - +0.5 225,853 - -1.0 226,163 + +0.4 226,220 + +0.2 226,221 - -1.2 226,231 + +0.8 226,231 + -0.3 226,231 + -1.8 226,231 + +0.3 226,232 - -1.8 226,232 - +1.1 226,452 + -0.4 226,452 + +2.2 226,581 + -1.2 226,628 + -0.4 226,628 + +0.5 226,628 + -0.5 226,628 + +1.3 226,628 + -0.0 226,628 + +0.8 226,628 + +0.3 226,629 - -0.3 226,629 - -2.2 226,629 - +0.0 226,629 - -0.2 226,629 - -0.0 226,632 + -1.6 226,632 + +0.2 226,733 + RR42_RS00950 0.13 -0.6 226,734 - RR42_RS00950 0.13 -2.0 226,919 + RR42_RS00950 0.32 +0.9 226,964 + RR42_RS00950 0.37 +0.9 226,965 - RR42_RS00950 0.37 +0.2 226,965 - RR42_RS00950 0.37 +0.9 226,965 - RR42_RS00950 0.37 +0.2 227,276 + RR42_RS00950 0.70 +1.7 227,277 - RR42_RS00950 0.70 +2.3 227,440 + RR42_RS00950 0.88 -1.1 227,441 - RR42_RS00950 0.88 +0.4 227,614 + -1.2 227,615 - +0.3 227,693 - +2.5 227,805 - RR42_RS00955 0.14 -3.6 227,901 + RR42_RS00955 0.24 +0.4 227,921 + RR42_RS00955 0.26 +0.4 227,922 - RR42_RS00955 0.27 +0.0 227,922 - RR42_RS00955 0.27 -0.0 228,158 - RR42_RS00955 0.52 -0.5 228,158 - RR42_RS00955 0.52 -0.8 228,307 - RR42_RS00955 0.68 +1.1 228,307 - RR42_RS00955 0.68 +1.1 228,441 + RR42_RS00955 0.82 -1.0 228,441 + RR42_RS00955 0.82 -0.5 228,442 - RR42_RS00955 0.82 -0.5 228,442 - RR42_RS00955 0.82 -1.4
Or see this region's nucleotide sequence