Experiment: R2A with Cobalt chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00335 and RR42_RS00340 are separated by 21 nucleotides RR42_RS00340 and RR42_RS00345 are separated by 152 nucleotides RR42_RS00345 and RR42_RS00350 are separated by 18 nucleotides
RR42_RS00335: RR42_RS00335 - MBL fold metallo-hydrolase, at 93,213 to 94,004
_RS00335
RR42_RS00340: RR42_RS00340 - glutathione S-transferase, at 94,026 to 94,652
_RS00340
RR42_RS00345: RR42_RS00345 - LysR family transcriptional regulator, at 94,805 to 95,716
_RS00345
RR42_RS00350: RR42_RS00350 - LysR family transcriptional regulator, at 95,735 to 96,637
_RS00350
Position (kb) 94
95
96 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 93.969 kb on + strand at 93.969 kb on + strand at 93.969 kb on + strand at 93.969 kb on + strand at 93.969 kb on + strand at 93.969 kb on + strand at 93.969 kb on + strand at 93.970 kb on - strand at 93.970 kb on - strand at 93.970 kb on - strand at 94.114 kb on + strand, within RR42_RS00340 at 94.115 kb on - strand, within RR42_RS00340 at 94.168 kb on + strand, within RR42_RS00340 at 94.168 kb on + strand, within RR42_RS00340 at 94.204 kb on + strand, within RR42_RS00340 at 94.204 kb on + strand, within RR42_RS00340 at 94.204 kb on + strand, within RR42_RS00340 at 94.205 kb on - strand, within RR42_RS00340 at 94.426 kb on + strand, within RR42_RS00340 at 94.426 kb on + strand, within RR42_RS00340 at 94.426 kb on + strand, within RR42_RS00340 at 94.426 kb on + strand, within RR42_RS00340 at 94.426 kb on + strand, within RR42_RS00340 at 94.427 kb on - strand, within RR42_RS00340 at 94.427 kb on - strand, within RR42_RS00340 at 94.504 kb on - strand, within RR42_RS00340 at 94.510 kb on + strand, within RR42_RS00340 at 94.510 kb on + strand, within RR42_RS00340 at 94.510 kb on + strand, within RR42_RS00340 at 94.511 kb on - strand, within RR42_RS00340 at 94.511 kb on - strand, within RR42_RS00340 at 94.639 kb on + strand at 94.639 kb on + strand at 94.639 kb on + strand at 94.640 kb on - strand at 94.642 kb on + strand at 94.643 kb on - strand at 94.735 kb on - strand at 94.812 kb on + strand at 94.813 kb on - strand at 94.818 kb on - strand at 94.985 kb on + strand, within RR42_RS00345 at 94.986 kb on - strand, within RR42_RS00345 at 95.714 kb on + strand at 95.714 kb on + strand at 95.790 kb on + strand at 95.908 kb on + strand, within RR42_RS00350 at 95.908 kb on + strand, within RR42_RS00350 at 95.910 kb on + strand, within RR42_RS00350 at 95.910 kb on + strand, within RR42_RS00350 at 95.910 kb on + strand, within RR42_RS00350 at 95.910 kb on + strand, within RR42_RS00350 at 95.910 kb on + strand, within RR42_RS00350 at 95.911 kb on - strand, within RR42_RS00350 at 96.118 kb on - strand, within RR42_RS00350 at 96.118 kb on - strand, within RR42_RS00350 at 96.136 kb on + strand, within RR42_RS00350 at 96.136 kb on + strand, within RR42_RS00350 at 96.136 kb on + strand, within RR42_RS00350 at 96.137 kb on - strand, within RR42_RS00350 at 96.137 kb on - strand, within RR42_RS00350 at 96.140 kb on + strand, within RR42_RS00350 at 96.140 kb on + strand, within RR42_RS00350 at 96.666 kb on + strand at 96.667 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.3 mM remove 93,969 + -1.7 93,969 + +2.0 93,969 + +0.3 93,969 + -0.2 93,969 + -0.0 93,969 + -0.0 93,969 + -1.9 93,970 - +0.3 93,970 - +0.7 93,970 - -0.5 94,114 + RR42_RS00340 0.14 -0.7 94,115 - RR42_RS00340 0.14 -0.2 94,168 + RR42_RS00340 0.23 -0.7 94,168 + RR42_RS00340 0.23 -1.1 94,204 + RR42_RS00340 0.28 -0.0 94,204 + RR42_RS00340 0.28 +0.1 94,204 + RR42_RS00340 0.28 +0.5 94,205 - RR42_RS00340 0.29 -1.5 94,426 + RR42_RS00340 0.64 -0.5 94,426 + RR42_RS00340 0.64 +0.4 94,426 + RR42_RS00340 0.64 -0.1 94,426 + RR42_RS00340 0.64 -0.6 94,426 + RR42_RS00340 0.64 +0.1 94,427 - RR42_RS00340 0.64 +0.1 94,427 - RR42_RS00340 0.64 +0.1 94,504 - RR42_RS00340 0.76 -1.1 94,510 + RR42_RS00340 0.77 -2.5 94,510 + RR42_RS00340 0.77 -1.7 94,510 + RR42_RS00340 0.77 -0.5 94,511 - RR42_RS00340 0.77 +0.6 94,511 - RR42_RS00340 0.77 -0.3 94,639 + -0.4 94,639 + +0.8 94,639 + +1.3 94,640 - +0.9 94,642 + -0.1 94,643 - -2.1 94,735 - -0.0 94,812 + +0.6 94,813 - +0.4 94,818 - +0.2 94,985 + RR42_RS00345 0.20 -0.1 94,986 - RR42_RS00345 0.20 -1.1 95,714 + -0.0 95,714 + +0.2 95,790 + -1.6 95,908 + RR42_RS00350 0.19 -0.7 95,908 + RR42_RS00350 0.19 +0.6 95,910 + RR42_RS00350 0.19 -0.8 95,910 + RR42_RS00350 0.19 +0.2 95,910 + RR42_RS00350 0.19 -2.1 95,910 + RR42_RS00350 0.19 +0.8 95,910 + RR42_RS00350 0.19 +0.2 95,911 - RR42_RS00350 0.19 -3.3 96,118 - RR42_RS00350 0.42 +0.2 96,118 - RR42_RS00350 0.42 +0.5 96,136 + RR42_RS00350 0.44 -1.2 96,136 + RR42_RS00350 0.44 +0.4 96,136 + RR42_RS00350 0.44 -0.5 96,137 - RR42_RS00350 0.45 +0.7 96,137 - RR42_RS00350 0.45 -1.2 96,140 + RR42_RS00350 0.45 -0.9 96,140 + RR42_RS00350 0.45 +0.5 96,666 + -1.3 96,667 - +0.3
Or see this region's nucleotide sequence