Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5625

Experiment: LB no stress 48 hours

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5623 and Pf6N2E2_5624 are separated by 1 nucleotidesPf6N2E2_5624 and Pf6N2E2_5625 are separated by 87 nucleotidesPf6N2E2_5625 and Pf6N2E2_5626 are separated by 13 nucleotidesPf6N2E2_5626 and Pf6N2E2_5627 are separated by 0 nucleotides Pf6N2E2_5623 - YciL protein, at 6,439,798 to 6,440,097 _5623 Pf6N2E2_5624 - Intracellular septation protein IspA, at 6,440,099 to 6,440,695 _5624 Pf6N2E2_5625 - COG0613, Predicted metal-dependent phosphoesterases (PHP family), at 6,440,783 to 6,441,646 _5625 Pf6N2E2_5626 - Hypothetical YciO protein, TsaC/YrdC paralog, at 6,441,660 to 6,442,289 _5626 Pf6N2E2_5627 - Segregation and condensation protein A, at 6,442,290 to 6,443,120 _5627 Position (kb) 6440 6441 6442Strain fitness (log2 ratio) -2 -1 0 1 2at 6439.800 kb on - strandat 6439.841 kb on + strand, within Pf6N2E2_5623at 6439.841 kb on + strand, within Pf6N2E2_5623at 6439.842 kb on - strand, within Pf6N2E2_5623at 6439.987 kb on + strand, within Pf6N2E2_5623at 6440.090 kb on + strandat 6440.090 kb on + strandat 6440.120 kb on - strandat 6440.727 kb on - strandat 6440.729 kb on + strandat 6440.729 kb on + strandat 6440.730 kb on - strandat 6440.730 kb on - strandat 6440.772 kb on - strandat 6440.806 kb on + strandat 6440.806 kb on + strandat 6440.807 kb on - strandat 6440.980 kb on + strand, within Pf6N2E2_5625at 6441.374 kb on + strand, within Pf6N2E2_5625at 6441.416 kb on + strand, within Pf6N2E2_5625at 6441.416 kb on + strand, within Pf6N2E2_5625at 6441.417 kb on - strand, within Pf6N2E2_5625at 6441.417 kb on - strand, within Pf6N2E2_5625at 6441.417 kb on - strand, within Pf6N2E2_5625at 6441.417 kb on - strand, within Pf6N2E2_5625at 6441.418 kb on + strand, within Pf6N2E2_5625at 6441.418 kb on + strand, within Pf6N2E2_5625at 6441.418 kb on + strand, within Pf6N2E2_5625at 6441.418 kb on + strand, within Pf6N2E2_5625at 6441.418 kb on + strand, within Pf6N2E2_5625at 6441.419 kb on - strand, within Pf6N2E2_5625at 6441.419 kb on - strand, within Pf6N2E2_5625at 6441.603 kb on + strandat 6441.603 kb on + strandat 6441.610 kb on + strandat 6441.610 kb on + strandat 6441.611 kb on - strandat 6441.611 kb on - strandat 6441.652 kb on + strandat 6441.653 kb on - strandat 6441.728 kb on + strand, within Pf6N2E2_5626at 6441.729 kb on - strand, within Pf6N2E2_5626at 6441.754 kb on - strand, within Pf6N2E2_5626at 6441.754 kb on - strand, within Pf6N2E2_5626at 6441.771 kb on + strand, within Pf6N2E2_5626at 6441.948 kb on + strand, within Pf6N2E2_5626at 6441.948 kb on + strand, within Pf6N2E2_5626at 6441.948 kb on + strand, within Pf6N2E2_5626at 6441.948 kb on + strand, within Pf6N2E2_5626at 6441.948 kb on + strand, within Pf6N2E2_5626at 6441.949 kb on - strand, within Pf6N2E2_5626at 6441.949 kb on - strand, within Pf6N2E2_5626at 6441.949 kb on - strand, within Pf6N2E2_5626at 6441.949 kb on - strand, within Pf6N2E2_5626at 6441.949 kb on - strand, within Pf6N2E2_5626at 6441.956 kb on + strand, within Pf6N2E2_5626at 6441.956 kb on + strand, within Pf6N2E2_5626at 6441.957 kb on - strand, within Pf6N2E2_5626at 6441.968 kb on + strand, within Pf6N2E2_5626at 6441.969 kb on - strand, within Pf6N2E2_5626at 6442.131 kb on + strand, within Pf6N2E2_5626at 6442.319 kb on + strandat 6442.368 kb on + strandat 6442.369 kb on - strandat 6442.404 kb on + strand, within Pf6N2E2_5627at 6442.404 kb on + strand, within Pf6N2E2_5627at 6442.405 kb on - strand, within Pf6N2E2_5627at 6442.405 kb on - strand, within Pf6N2E2_5627at 6442.470 kb on + strand, within Pf6N2E2_5627at 6442.470 kb on + strand, within Pf6N2E2_5627at 6442.470 kb on + strand, within Pf6N2E2_5627at 6442.533 kb on + strand, within Pf6N2E2_5627at 6442.533 kb on + strand, within Pf6N2E2_5627at 6442.534 kb on - strand, within Pf6N2E2_5627at 6442.534 kb on - strand, within Pf6N2E2_5627at 6442.543 kb on - strand, within Pf6N2E2_5627at 6442.543 kb on - strand, within Pf6N2E2_5627at 6442.543 kb on - strand, within Pf6N2E2_5627at 6442.587 kb on + strand, within Pf6N2E2_5627

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Per-strain Table

Position Strand Gene LocusTag Fraction LB no stress 48 hours
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6,439,800 - +0.5
6,439,841 + Pf6N2E2_5623 0.14 -2.2
6,439,841 + Pf6N2E2_5623 0.14 +0.9
6,439,842 - Pf6N2E2_5623 0.15 +0.2
6,439,987 + Pf6N2E2_5623 0.63 +0.4
6,440,090 + +1.6
6,440,090 + -1.6
6,440,120 - +0.9
6,440,727 - -0.5
6,440,729 + -0.4
6,440,729 + +0.3
6,440,730 - -0.4
6,440,730 - +0.3
6,440,772 - -0.9
6,440,806 + -2.2
6,440,806 + +0.8
6,440,807 - -0.3
6,440,980 + Pf6N2E2_5625 0.23 -1.4
6,441,374 + Pf6N2E2_5625 0.68 +1.9
6,441,416 + Pf6N2E2_5625 0.73 +0.3
6,441,416 + Pf6N2E2_5625 0.73 +1.1
6,441,417 - Pf6N2E2_5625 0.73 +0.6
6,441,417 - Pf6N2E2_5625 0.73 +2.3
6,441,417 - Pf6N2E2_5625 0.73 +1.8
6,441,417 - Pf6N2E2_5625 0.73 +0.0
6,441,418 + Pf6N2E2_5625 0.73 -0.5
6,441,418 + Pf6N2E2_5625 0.73 -0.2
6,441,418 + Pf6N2E2_5625 0.73 +0.2
6,441,418 + Pf6N2E2_5625 0.73 +0.6
6,441,418 + Pf6N2E2_5625 0.73 -0.2
6,441,419 - Pf6N2E2_5625 0.74 +1.1
6,441,419 - Pf6N2E2_5625 0.74 +0.8
6,441,603 + +0.3
6,441,603 + +0.5
6,441,610 + +0.8
6,441,610 + -0.2
6,441,611 - -0.4
6,441,611 - +0.5
6,441,652 + +0.1
6,441,653 - +0.8
6,441,728 + Pf6N2E2_5626 0.11 -1.5
6,441,729 - Pf6N2E2_5626 0.11 +0.9
6,441,754 - Pf6N2E2_5626 0.15 +0.9
6,441,754 - Pf6N2E2_5626 0.15 -0.8
6,441,771 + Pf6N2E2_5626 0.18 +0.1
6,441,948 + Pf6N2E2_5626 0.46 +0.6
6,441,948 + Pf6N2E2_5626 0.46 -1.1
6,441,948 + Pf6N2E2_5626 0.46 +2.0
6,441,948 + Pf6N2E2_5626 0.46 -0.0
6,441,948 + Pf6N2E2_5626 0.46 -0.0
6,441,949 - Pf6N2E2_5626 0.46 -0.1
6,441,949 - Pf6N2E2_5626 0.46 +1.1
6,441,949 - Pf6N2E2_5626 0.46 +1.0
6,441,949 - Pf6N2E2_5626 0.46 -0.8
6,441,949 - Pf6N2E2_5626 0.46 -0.2
6,441,956 + Pf6N2E2_5626 0.47 -1.9
6,441,956 + Pf6N2E2_5626 0.47 +1.9
6,441,957 - Pf6N2E2_5626 0.47 -2.2
6,441,968 + Pf6N2E2_5626 0.49 +1.1
6,441,969 - Pf6N2E2_5626 0.49 -0.1
6,442,131 + Pf6N2E2_5626 0.75 +0.8
6,442,319 + +0.5
6,442,368 + +1.9
6,442,369 - -0.7
6,442,404 + Pf6N2E2_5627 0.14 -1.8
6,442,404 + Pf6N2E2_5627 0.14 +0.1
6,442,405 - Pf6N2E2_5627 0.14 -0.3
6,442,405 - Pf6N2E2_5627 0.14 +0.9
6,442,470 + Pf6N2E2_5627 0.22 +1.1
6,442,470 + Pf6N2E2_5627 0.22 +0.1
6,442,470 + Pf6N2E2_5627 0.22 -0.2
6,442,533 + Pf6N2E2_5627 0.29 -0.7
6,442,533 + Pf6N2E2_5627 0.29 +0.5
6,442,534 - Pf6N2E2_5627 0.29 -2.2
6,442,534 - Pf6N2E2_5627 0.29 -0.7
6,442,543 - Pf6N2E2_5627 0.30 -0.2
6,442,543 - Pf6N2E2_5627 0.30 -0.2
6,442,543 - Pf6N2E2_5627 0.30 +0.7
6,442,587 + Pf6N2E2_5627 0.36 -1.9

Or see this region's nucleotide sequence