Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5417 and Pf6N2E2_5418 are separated by 261 nucleotides Pf6N2E2_5418 and Pf6N2E2_5419 are separated by 8 nucleotides Pf6N2E2_5419 and Pf6N2E2_5420 are separated by 60 nucleotides
Pf6N2E2_5417 - Valyl-tRNA synthetase (EC 6.1.1.9), at 6,220,443 to 6,223,289
_5417
Pf6N2E2_5418 - FIG00957284: hypothetical protein, at 6,223,551 to 6,223,916
_5418
Pf6N2E2_5419 - DNA polymerase III chi subunit (EC 2.7.7.7), at 6,223,925 to 6,224,353
_5419
Pf6N2E2_5420 - Cytosol aminopeptidase PepA (EC 3.4.11.1), at 6,224,414 to 6,225,904
_5420
Position (kb)
6223
6224
6225 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6223.336 kb on - strand at 6223.336 kb on - strand at 6223.336 kb on - strand at 6223.382 kb on + strand at 6223.383 kb on - strand at 6223.386 kb on - strand at 6223.552 kb on + strand at 6223.553 kb on - strand at 6223.553 kb on - strand at 6223.553 kb on - strand at 6223.558 kb on + strand at 6223.558 kb on + strand at 6223.559 kb on - strand at 6223.559 kb on - strand at 6223.919 kb on + strand at 6223.937 kb on + strand at 6223.938 kb on - strand at 6224.247 kb on + strand, within Pf6N2E2_5419 at 6224.247 kb on + strand, within Pf6N2E2_5419 at 6224.248 kb on - strand, within Pf6N2E2_5419 at 6224.248 kb on - strand, within Pf6N2E2_5419 at 6224.248 kb on - strand, within Pf6N2E2_5419 at 6224.248 kb on - strand, within Pf6N2E2_5419 at 6224.334 kb on + strand at 6224.334 kb on + strand at 6224.335 kb on - strand at 6224.335 kb on - strand at 6224.335 kb on - strand at 6224.335 kb on - strand at 6224.335 kb on - strand at 6224.355 kb on - strand at 6224.439 kb on + strand at 6224.641 kb on - strand, within Pf6N2E2_5420 at 6224.641 kb on - strand, within Pf6N2E2_5420 at 6224.824 kb on - strand, within Pf6N2E2_5420 at 6224.824 kb on - strand, within Pf6N2E2_5420 at 6224.824 kb on - strand, within Pf6N2E2_5420 at 6225.043 kb on - strand, within Pf6N2E2_5420 at 6225.043 kb on - strand, within Pf6N2E2_5420 at 6225.043 kb on - strand, within Pf6N2E2_5420 at 6225.043 kb on - strand, within Pf6N2E2_5420 at 6225.153 kb on + strand, within Pf6N2E2_5420 at 6225.154 kb on - strand, within Pf6N2E2_5420 at 6225.154 kb on - strand, within Pf6N2E2_5420 at 6225.154 kb on - strand, within Pf6N2E2_5420 at 6225.204 kb on + strand, within Pf6N2E2_5420
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 6,223,336 - -0.5 6,223,336 - +0.8 6,223,336 - -0.1 6,223,382 + -0.8 6,223,383 - +0.8 6,223,386 - +0.0 6,223,552 + +1.0 6,223,553 - +0.4 6,223,553 - +0.0 6,223,553 - +0.4 6,223,558 + -2.4 6,223,558 + +0.9 6,223,559 - +0.9 6,223,559 - +0.5 6,223,919 + +0.3 6,223,937 + +0.5 6,223,938 - +0.2 6,224,247 + Pf6N2E2_5419 0.75 -0.8 6,224,247 + Pf6N2E2_5419 0.75 -1.2 6,224,248 - Pf6N2E2_5419 0.75 -1.6 6,224,248 - Pf6N2E2_5419 0.75 +0.6 6,224,248 - Pf6N2E2_5419 0.75 -2.0 6,224,248 - Pf6N2E2_5419 0.75 +0.2 6,224,334 + -1.3 6,224,334 + +1.1 6,224,335 - -0.8 6,224,335 - +1.7 6,224,335 - +0.3 6,224,335 - -0.4 6,224,335 - -0.8 6,224,355 - +0.2 6,224,439 + +0.3 6,224,641 - Pf6N2E2_5420 0.15 +0.7 6,224,641 - Pf6N2E2_5420 0.15 -0.3 6,224,824 - Pf6N2E2_5420 0.27 -0.4 6,224,824 - Pf6N2E2_5420 0.27 -0.2 6,224,824 - Pf6N2E2_5420 0.27 -0.5 6,225,043 - Pf6N2E2_5420 0.42 -0.0 6,225,043 - Pf6N2E2_5420 0.42 -0.6 6,225,043 - Pf6N2E2_5420 0.42 -2.9 6,225,043 - Pf6N2E2_5420 0.42 -0.4 6,225,153 + Pf6N2E2_5420 0.50 -0.1 6,225,154 - Pf6N2E2_5420 0.50 +0.4 6,225,154 - Pf6N2E2_5420 0.50 +1.9 6,225,154 - Pf6N2E2_5420 0.50 +0.6 6,225,204 + Pf6N2E2_5420 0.53 -1.1
Or see this region's nucleotide sequence