Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_507

Experiment: LB no stress 48 hours

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_504 and Pf6N2E2_505 are separated by 24 nucleotidesPf6N2E2_505 and Pf6N2E2_506 overlap by 35 nucleotidesPf6N2E2_506 and Pf6N2E2_507 overlap by 4 nucleotidesPf6N2E2_507 and Pf6N2E2_508 overlap by 8 nucleotides Pf6N2E2_504 - DNA-binding response regulator, at 574,064 to 574,747 _504 Pf6N2E2_505 - hypothetical protein, at 574,772 to 575,053 _505 Pf6N2E2_506 - Hydrogen cyanide synthase HcnA / Opine oxidase subunit C, at 575,019 to 575,342 _506 Pf6N2E2_507 - Hydrogen cyanide synthase HcnB / Opine oxidase subunit A, at 575,339 to 576,748 _507 Pf6N2E2_508 - Hydrogen cyanide synthase HcnC / Opine oxidase subunit B, at 576,741 to 578,003 _508 Position (kb) 575 576 577Strain fitness (log2 ratio) -2 -1 0 1 2at 574.361 kb on + strand, within Pf6N2E2_504at 574.612 kb on - strand, within Pf6N2E2_504at 574.776 kb on + strandat 574.776 kb on + strandat 574.802 kb on - strand, within Pf6N2E2_505at 574.878 kb on + strand, within Pf6N2E2_505at 574.878 kb on + strand, within Pf6N2E2_505at 574.878 kb on + strand, within Pf6N2E2_505at 574.890 kb on + strand, within Pf6N2E2_505at 574.932 kb on + strand, within Pf6N2E2_505at 574.933 kb on - strand, within Pf6N2E2_505at 575.015 kb on + strand, within Pf6N2E2_505at 575.015 kb on + strand, within Pf6N2E2_505at 575.015 kb on + strand, within Pf6N2E2_505at 575.015 kb on + strand, within Pf6N2E2_505at 575.016 kb on - strand, within Pf6N2E2_505at 575.016 kb on - strand, within Pf6N2E2_505at 575.016 kb on - strand, within Pf6N2E2_505at 575.016 kb on - strand, within Pf6N2E2_505at 575.038 kb on + strandat 575.038 kb on + strandat 575.039 kb on - strandat 575.202 kb on + strand, within Pf6N2E2_506at 575.202 kb on + strand, within Pf6N2E2_506at 575.218 kb on + strand, within Pf6N2E2_506at 575.219 kb on - strand, within Pf6N2E2_506at 575.219 kb on - strand, within Pf6N2E2_506at 575.219 kb on - strand, within Pf6N2E2_506at 575.219 kb on - strand, within Pf6N2E2_506at 575.219 kb on - strand, within Pf6N2E2_506at 575.498 kb on + strand, within Pf6N2E2_507at 575.876 kb on + strand, within Pf6N2E2_507at 575.876 kb on + strand, within Pf6N2E2_507at 575.876 kb on + strand, within Pf6N2E2_507at 575.876 kb on + strand, within Pf6N2E2_507at 575.876 kb on + strand, within Pf6N2E2_507at 575.877 kb on - strand, within Pf6N2E2_507at 575.877 kb on - strand, within Pf6N2E2_507at 575.877 kb on - strand, within Pf6N2E2_507at 575.877 kb on - strand, within Pf6N2E2_507at 575.877 kb on - strand, within Pf6N2E2_507at 575.877 kb on - strand, within Pf6N2E2_507at 575.877 kb on - strand, within Pf6N2E2_507at 576.000 kb on - strand, within Pf6N2E2_507at 576.201 kb on - strand, within Pf6N2E2_507at 576.384 kb on - strand, within Pf6N2E2_507at 576.746 kb on + strandat 576.746 kb on + strandat 576.747 kb on - strandat 576.747 kb on - strandat 576.747 kb on - strandat 576.747 kb on - strandat 576.753 kb on + strandat 576.754 kb on - strandat 576.801 kb on + strandat 576.802 kb on - strandat 577.011 kb on - strand, within Pf6N2E2_508at 577.060 kb on - strand, within Pf6N2E2_508at 577.060 kb on - strand, within Pf6N2E2_508at 577.060 kb on - strand, within Pf6N2E2_508at 577.161 kb on + strand, within Pf6N2E2_508at 577.162 kb on - strand, within Pf6N2E2_508at 577.326 kb on + strand, within Pf6N2E2_508at 577.327 kb on - strand, within Pf6N2E2_508at 577.327 kb on - strand, within Pf6N2E2_508at 577.327 kb on - strand, within Pf6N2E2_508at 577.327 kb on - strand, within Pf6N2E2_508at 577.602 kb on + strand, within Pf6N2E2_508at 577.603 kb on - strand, within Pf6N2E2_508at 577.603 kb on - strand, within Pf6N2E2_508at 577.603 kb on - strand, within Pf6N2E2_508at 577.603 kb on - strand, within Pf6N2E2_508at 577.715 kb on - strand, within Pf6N2E2_508at 577.721 kb on + strand, within Pf6N2E2_508at 577.721 kb on + strand, within Pf6N2E2_508

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Per-strain Table

Position Strand Gene LocusTag Fraction LB no stress 48 hours
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574,361 + Pf6N2E2_504 0.43 +0.5
574,612 - Pf6N2E2_504 0.80 -1.2
574,776 + +0.2
574,776 + -0.6
574,802 - Pf6N2E2_505 0.11 -1.3
574,878 + Pf6N2E2_505 0.38 +0.5
574,878 + Pf6N2E2_505 0.38 +2.1
574,878 + Pf6N2E2_505 0.38 +0.5
574,890 + Pf6N2E2_505 0.42 +1.9
574,932 + Pf6N2E2_505 0.57 +0.7
574,933 - Pf6N2E2_505 0.57 +0.2
575,015 + Pf6N2E2_505 0.86 -0.4
575,015 + Pf6N2E2_505 0.86 +0.5
575,015 + Pf6N2E2_505 0.86 +1.1
575,015 + Pf6N2E2_505 0.86 +1.0
575,016 - Pf6N2E2_505 0.87 -0.2
575,016 - Pf6N2E2_505 0.87 +0.5
575,016 - Pf6N2E2_505 0.87 +0.0
575,016 - Pf6N2E2_505 0.87 -1.2
575,038 + -2.6
575,038 + +0.1
575,039 - -0.9
575,202 + Pf6N2E2_506 0.56 +1.5
575,202 + Pf6N2E2_506 0.56 -1.0
575,218 + Pf6N2E2_506 0.61 -0.3
575,219 - Pf6N2E2_506 0.62 -1.0
575,219 - Pf6N2E2_506 0.62 -1.2
575,219 - Pf6N2E2_506 0.62 +0.4
575,219 - Pf6N2E2_506 0.62 -1.0
575,219 - Pf6N2E2_506 0.62 -0.5
575,498 + Pf6N2E2_507 0.11 +0.7
575,876 + Pf6N2E2_507 0.38 +0.1
575,876 + Pf6N2E2_507 0.38 -0.1
575,876 + Pf6N2E2_507 0.38 -0.6
575,876 + Pf6N2E2_507 0.38 +1.5
575,876 + Pf6N2E2_507 0.38 +1.5
575,877 - Pf6N2E2_507 0.38 -1.9
575,877 - Pf6N2E2_507 0.38 +0.1
575,877 - Pf6N2E2_507 0.38 +1.0
575,877 - Pf6N2E2_507 0.38 +0.5
575,877 - Pf6N2E2_507 0.38 +1.8
575,877 - Pf6N2E2_507 0.38 +1.5
575,877 - Pf6N2E2_507 0.38 +0.8
576,000 - Pf6N2E2_507 0.47 +1.1
576,201 - Pf6N2E2_507 0.61 -0.3
576,384 - Pf6N2E2_507 0.74 -0.0
576,746 + +0.1
576,746 + -1.5
576,747 - +0.1
576,747 - +1.2
576,747 - +0.2
576,747 - -1.2
576,753 + +0.5
576,754 - -0.7
576,801 + +0.1
576,802 - -0.7
577,011 - Pf6N2E2_508 0.21 -0.5
577,060 - Pf6N2E2_508 0.25 +1.5
577,060 - Pf6N2E2_508 0.25 +0.2
577,060 - Pf6N2E2_508 0.25 +1.1
577,161 + Pf6N2E2_508 0.33 +0.1
577,162 - Pf6N2E2_508 0.33 +0.4
577,326 + Pf6N2E2_508 0.46 +0.0
577,327 - Pf6N2E2_508 0.46 +1.9
577,327 - Pf6N2E2_508 0.46 -0.5
577,327 - Pf6N2E2_508 0.46 +0.6
577,327 - Pf6N2E2_508 0.46 +0.1
577,602 + Pf6N2E2_508 0.68 +1.2
577,603 - Pf6N2E2_508 0.68 +0.1
577,603 - Pf6N2E2_508 0.68 +0.7
577,603 - Pf6N2E2_508 0.68 +0.2
577,603 - Pf6N2E2_508 0.68 +0.3
577,715 - Pf6N2E2_508 0.77 -1.2
577,721 + Pf6N2E2_508 0.78 +0.7
577,721 + Pf6N2E2_508 0.78 +1.0

Or see this region's nucleotide sequence