Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4591 and Pf6N2E2_4592 are separated by 144 nucleotides Pf6N2E2_4592 and Pf6N2E2_4593 are separated by 21 nucleotides Pf6N2E2_4593 and Pf6N2E2_4594 are separated by 62 nucleotides Pf6N2E2_4594 and Pf6N2E2_4595 overlap by 4 nucleotides
Pf6N2E2_4591 - tRNA (guanine46-N7-)-methyltransferase (EC 2.1.1.33), at 5,319,902 to 5,320,627
_4591
Pf6N2E2_4592 - Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific, at 5,320,772 to 5,321,095
_4592
Pf6N2E2_4593 - Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB, at 5,321,117 to 5,322,250
_4593
Pf6N2E2_4594 - Nucleoside 5-triphosphatase RdgB (dHAPTP, dITP, XTP-specific) (EC 3.6.1.15), at 5,322,313 to 5,322,909
_4594
Pf6N2E2_4595 - FIG003551: hypothetical protein, at 5,322,906 to 5,323,340
_4595
Position (kb)
5321
5322
5323 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5320.457 kb on + strand, within Pf6N2E2_4591 at 5320.457 kb on + strand, within Pf6N2E2_4591 at 5320.457 kb on + strand, within Pf6N2E2_4591 at 5320.457 kb on + strand, within Pf6N2E2_4591 at 5320.458 kb on - strand, within Pf6N2E2_4591 at 5320.458 kb on - strand, within Pf6N2E2_4591 at 5320.466 kb on + strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.626 kb on - strand at 5320.690 kb on + strand at 5320.690 kb on + strand at 5320.691 kb on - strand at 5320.691 kb on - strand at 5320.716 kb on + strand at 5320.836 kb on + strand, within Pf6N2E2_4592 at 5320.836 kb on + strand, within Pf6N2E2_4592 at 5320.836 kb on + strand, within Pf6N2E2_4592 at 5320.836 kb on + strand, within Pf6N2E2_4592 at 5320.836 kb on + strand, within Pf6N2E2_4592 at 5320.836 kb on + strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.881 kb on + strand, within Pf6N2E2_4592 at 5320.881 kb on + strand, within Pf6N2E2_4592 at 5320.881 kb on + strand, within Pf6N2E2_4592 at 5320.882 kb on - strand, within Pf6N2E2_4592 at 5320.882 kb on - strand, within Pf6N2E2_4592 at 5320.882 kb on - strand, within Pf6N2E2_4592 at 5320.882 kb on - strand, within Pf6N2E2_4592 at 5320.931 kb on + strand, within Pf6N2E2_4592 at 5321.115 kb on - strand at 5321.118 kb on + strand at 5321.118 kb on + strand at 5321.118 kb on + strand at 5321.119 kb on - strand at 5321.119 kb on - strand at 5321.119 kb on - strand at 5321.119 kb on - strand at 5321.119 kb on - strand at 5321.388 kb on + strand, within Pf6N2E2_4593 at 5321.388 kb on + strand, within Pf6N2E2_4593 at 5321.388 kb on + strand, within Pf6N2E2_4593 at 5321.389 kb on - strand, within Pf6N2E2_4593 at 5321.420 kb on + strand, within Pf6N2E2_4593 at 5321.421 kb on - strand, within Pf6N2E2_4593 at 5321.496 kb on + strand, within Pf6N2E2_4593 at 5321.496 kb on + strand, within Pf6N2E2_4593 at 5321.497 kb on - strand, within Pf6N2E2_4593 at 5321.506 kb on + strand, within Pf6N2E2_4593 at 5321.550 kb on + strand, within Pf6N2E2_4593 at 5322.141 kb on + strand at 5322.141 kb on + strand at 5322.142 kb on - strand at 5322.142 kb on - strand at 5322.142 kb on - strand at 5322.142 kb on - strand at 5322.142 kb on - strand at 5322.142 kb on - strand at 5322.142 kb on - strand at 5322.228 kb on + strand at 5322.228 kb on + strand at 5322.228 kb on + strand at 5322.228 kb on + strand at 5322.229 kb on - strand at 5322.229 kb on - strand at 5322.229 kb on - strand at 5322.653 kb on + strand, within Pf6N2E2_4594 at 5322.654 kb on - strand, within Pf6N2E2_4594 at 5322.654 kb on - strand, within Pf6N2E2_4594 at 5322.654 kb on - strand, within Pf6N2E2_4594 at 5322.820 kb on + strand, within Pf6N2E2_4594 at 5322.886 kb on + strand at 5322.887 kb on - strand at 5323.021 kb on + strand, within Pf6N2E2_4595 at 5323.021 kb on + strand, within Pf6N2E2_4595 at 5323.021 kb on + strand, within Pf6N2E2_4595 at 5323.021 kb on + strand, within Pf6N2E2_4595 at 5323.021 kb on + strand, within Pf6N2E2_4595 at 5323.021 kb on + strand, within Pf6N2E2_4595 at 5323.022 kb on - strand, within Pf6N2E2_4595 at 5323.022 kb on - strand, within Pf6N2E2_4595 at 5323.022 kb on - strand, within Pf6N2E2_4595 at 5323.022 kb on - strand, within Pf6N2E2_4595 at 5323.024 kb on + strand, within Pf6N2E2_4595 at 5323.025 kb on - strand, within Pf6N2E2_4595 at 5323.025 kb on - strand, within Pf6N2E2_4595 at 5323.025 kb on - strand, within Pf6N2E2_4595 at 5323.025 kb on - strand, within Pf6N2E2_4595 at 5323.228 kb on + strand, within Pf6N2E2_4595 at 5323.228 kb on + strand, within Pf6N2E2_4595 at 5323.228 kb on + strand, within Pf6N2E2_4595 at 5323.228 kb on + strand, within Pf6N2E2_4595 at 5323.228 kb on + strand, within Pf6N2E2_4595 at 5323.228 kb on + strand, within Pf6N2E2_4595 at 5323.229 kb on - strand, within Pf6N2E2_4595 at 5323.229 kb on - strand, within Pf6N2E2_4595 at 5323.229 kb on - strand, within Pf6N2E2_4595 at 5323.229 kb on - strand, within Pf6N2E2_4595 at 5323.242 kb on - strand, within Pf6N2E2_4595
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 5,320,457 + Pf6N2E2_4591 0.76 -0.6 5,320,457 + Pf6N2E2_4591 0.76 +0.4 5,320,457 + Pf6N2E2_4591 0.76 -1.0 5,320,457 + Pf6N2E2_4591 0.76 +0.1 5,320,458 - Pf6N2E2_4591 0.77 -0.5 5,320,458 - Pf6N2E2_4591 0.77 -0.3 5,320,466 + Pf6N2E2_4591 0.78 -1.5 5,320,467 - Pf6N2E2_4591 0.78 -0.8 5,320,467 - Pf6N2E2_4591 0.78 +0.4 5,320,467 - Pf6N2E2_4591 0.78 -0.2 5,320,467 - Pf6N2E2_4591 0.78 +0.6 5,320,467 - Pf6N2E2_4591 0.78 -0.6 5,320,467 - Pf6N2E2_4591 0.78 +1.5 5,320,467 - Pf6N2E2_4591 0.78 -0.6 5,320,626 - -0.4 5,320,690 + -0.3 5,320,690 + +0.8 5,320,691 - +2.0 5,320,691 - -1.1 5,320,716 + -0.0 5,320,836 + Pf6N2E2_4592 0.20 -0.2 5,320,836 + Pf6N2E2_4592 0.20 -0.5 5,320,836 + Pf6N2E2_4592 0.20 +0.6 5,320,836 + Pf6N2E2_4592 0.20 -0.4 5,320,836 + Pf6N2E2_4592 0.20 +0.1 5,320,836 + Pf6N2E2_4592 0.20 -0.6 5,320,837 - Pf6N2E2_4592 0.20 -2.4 5,320,837 - Pf6N2E2_4592 0.20 -1.0 5,320,837 - Pf6N2E2_4592 0.20 -3.2 5,320,837 - Pf6N2E2_4592 0.20 -1.9 5,320,837 - Pf6N2E2_4592 0.20 -0.6 5,320,837 - Pf6N2E2_4592 0.20 +0.1 5,320,837 - Pf6N2E2_4592 0.20 -1.0 5,320,837 - Pf6N2E2_4592 0.20 +0.4 5,320,881 + Pf6N2E2_4592 0.34 +0.8 5,320,881 + Pf6N2E2_4592 0.34 -2.1 5,320,881 + Pf6N2E2_4592 0.34 +1.2 5,320,882 - Pf6N2E2_4592 0.34 +0.6 5,320,882 - Pf6N2E2_4592 0.34 -0.6 5,320,882 - Pf6N2E2_4592 0.34 +0.5 5,320,882 - Pf6N2E2_4592 0.34 -0.2 5,320,931 + Pf6N2E2_4592 0.49 +0.4 5,321,115 - +1.0 5,321,118 + +1.5 5,321,118 + +1.4 5,321,118 + -0.1 5,321,119 - -0.6 5,321,119 - +0.4 5,321,119 - -0.4 5,321,119 - -1.3 5,321,119 - +1.3 5,321,388 + Pf6N2E2_4593 0.24 -0.1 5,321,388 + Pf6N2E2_4593 0.24 -2.0 5,321,388 + Pf6N2E2_4593 0.24 +0.2 5,321,389 - Pf6N2E2_4593 0.24 -0.4 5,321,420 + Pf6N2E2_4593 0.27 -1.0 5,321,421 - Pf6N2E2_4593 0.27 +0.2 5,321,496 + Pf6N2E2_4593 0.33 -0.0 5,321,496 + Pf6N2E2_4593 0.33 -1.4 5,321,497 - Pf6N2E2_4593 0.34 -2.1 5,321,506 + Pf6N2E2_4593 0.34 -0.2 5,321,550 + Pf6N2E2_4593 0.38 +0.4 5,322,141 + +1.2 5,322,141 + +0.7 5,322,142 - -0.2 5,322,142 - +0.1 5,322,142 - +0.6 5,322,142 - -0.6 5,322,142 - -1.2 5,322,142 - +1.6 5,322,142 - +0.3 5,322,228 + +0.6 5,322,228 + +3.7 5,322,228 + -2.5 5,322,228 + +1.0 5,322,229 - -0.4 5,322,229 - +1.2 5,322,229 - -0.7 5,322,653 + Pf6N2E2_4594 0.57 +0.1 5,322,654 - Pf6N2E2_4594 0.57 -0.4 5,322,654 - Pf6N2E2_4594 0.57 +0.6 5,322,654 - Pf6N2E2_4594 0.57 -0.5 5,322,820 + Pf6N2E2_4594 0.85 -2.0 5,322,886 + +0.7 5,322,887 - -0.1 5,323,021 + Pf6N2E2_4595 0.26 -2.1 5,323,021 + Pf6N2E2_4595 0.26 -0.5 5,323,021 + Pf6N2E2_4595 0.26 -0.9 5,323,021 + Pf6N2E2_4595 0.26 -0.9 5,323,021 + Pf6N2E2_4595 0.26 -2.9 5,323,021 + Pf6N2E2_4595 0.26 +1.3 5,323,022 - Pf6N2E2_4595 0.27 +0.5 5,323,022 - Pf6N2E2_4595 0.27 -0.1 5,323,022 - Pf6N2E2_4595 0.27 +0.3 5,323,022 - Pf6N2E2_4595 0.27 -0.5 5,323,024 + Pf6N2E2_4595 0.27 -2.7 5,323,025 - Pf6N2E2_4595 0.27 -0.3 5,323,025 - Pf6N2E2_4595 0.27 +1.2 5,323,025 - Pf6N2E2_4595 0.27 -2.3 5,323,025 - Pf6N2E2_4595 0.27 +0.1 5,323,228 + Pf6N2E2_4595 0.74 +0.4 5,323,228 + Pf6N2E2_4595 0.74 +0.5 5,323,228 + Pf6N2E2_4595 0.74 -1.0 5,323,228 + Pf6N2E2_4595 0.74 +1.2 5,323,228 + Pf6N2E2_4595 0.74 -0.9 5,323,228 + Pf6N2E2_4595 0.74 +1.0 5,323,229 - Pf6N2E2_4595 0.74 -1.2 5,323,229 - Pf6N2E2_4595 0.74 +0.3 5,323,229 - Pf6N2E2_4595 0.74 +0.3 5,323,229 - Pf6N2E2_4595 0.74 -0.6 5,323,242 - Pf6N2E2_4595 0.77 -0.3
Or see this region's nucleotide sequence