Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4551 and Pf6N2E2_4552 are separated by 293 nucleotides Pf6N2E2_4552 and Pf6N2E2_4553 are separated by 93 nucleotides Pf6N2E2_4553 and Pf6N2E2_4554 are separated by 403 nucleotides
Pf6N2E2_4551 - hypothetical protein, at 5,281,073 to 5,281,750
_4551
Pf6N2E2_4552 - hypothetical protein, at 5,282,044 to 5,282,253
_4552
Pf6N2E2_4553 - Nitrogen regulation protein NtrC, at 5,282,347 to 5,283,270
_4553
Pf6N2E2_4554 - Thiol-disulfide isomerase and thioredoxins, at 5,283,674 to 5,284,249
_4554
Position (kb)
5282
5283
5284 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5281.668 kb on + strand, within Pf6N2E2_4551 at 5281.668 kb on + strand, within Pf6N2E2_4551 at 5281.669 kb on - strand, within Pf6N2E2_4551 at 5281.669 kb on - strand, within Pf6N2E2_4551 at 5281.739 kb on + strand at 5282.053 kb on + strand at 5282.053 kb on + strand at 5282.053 kb on + strand at 5282.053 kb on + strand at 5282.253 kb on + strand at 5282.254 kb on - strand at 5282.254 kb on - strand at 5282.259 kb on + strand at 5282.260 kb on - strand at 5282.260 kb on - strand at 5282.307 kb on + strand at 5282.359 kb on + strand at 5282.359 kb on + strand at 5282.384 kb on + strand at 5282.384 kb on + strand at 5282.385 kb on - strand at 5282.385 kb on - strand at 5282.385 kb on - strand at 5282.560 kb on + strand, within Pf6N2E2_4553 at 5282.560 kb on + strand, within Pf6N2E2_4553 at 5282.560 kb on + strand, within Pf6N2E2_4553 at 5282.561 kb on - strand, within Pf6N2E2_4553 at 5282.561 kb on - strand, within Pf6N2E2_4553 at 5282.654 kb on + strand, within Pf6N2E2_4553 at 5282.771 kb on + strand, within Pf6N2E2_4553 at 5282.771 kb on + strand, within Pf6N2E2_4553 at 5282.954 kb on + strand, within Pf6N2E2_4553 at 5282.954 kb on + strand, within Pf6N2E2_4553 at 5282.967 kb on + strand, within Pf6N2E2_4553 at 5282.968 kb on - strand, within Pf6N2E2_4553 at 5282.968 kb on - strand, within Pf6N2E2_4553 at 5283.012 kb on + strand, within Pf6N2E2_4553 at 5283.233 kb on + strand at 5283.233 kb on + strand at 5283.233 kb on + strand at 5283.376 kb on - strand at 5283.376 kb on - strand at 5283.378 kb on + strand at 5283.379 kb on - strand at 5283.404 kb on - strand at 5283.416 kb on - strand at 5283.461 kb on - strand at 5283.583 kb on + strand at 5283.583 kb on + strand at 5283.583 kb on + strand at 5283.583 kb on + strand at 5283.601 kb on + strand at 5283.614 kb on + strand at 5283.614 kb on + strand at 5283.614 kb on + strand at 5283.614 kb on + strand at 5283.614 kb on + strand at 5283.615 kb on - strand at 5283.615 kb on - strand at 5283.655 kb on - strand at 5283.664 kb on - strand at 5283.667 kb on + strand at 5283.730 kb on + strand at 5283.731 kb on - strand at 5283.811 kb on - strand, within Pf6N2E2_4554 at 5283.822 kb on + strand, within Pf6N2E2_4554 at 5283.964 kb on - strand, within Pf6N2E2_4554 at 5283.983 kb on + strand, within Pf6N2E2_4554 at 5283.983 kb on + strand, within Pf6N2E2_4554 at 5283.983 kb on + strand, within Pf6N2E2_4554 at 5283.983 kb on + strand, within Pf6N2E2_4554 at 5283.987 kb on + strand, within Pf6N2E2_4554 at 5283.987 kb on + strand, within Pf6N2E2_4554 at 5283.987 kb on + strand, within Pf6N2E2_4554 at 5283.987 kb on + strand, within Pf6N2E2_4554 at 5283.987 kb on + strand, within Pf6N2E2_4554 at 5283.987 kb on + strand, within Pf6N2E2_4554 at 5283.987 kb on + strand, within Pf6N2E2_4554 at 5283.987 kb on + strand, within Pf6N2E2_4554 at 5283.987 kb on + strand, within Pf6N2E2_4554 at 5283.987 kb on + strand, within Pf6N2E2_4554 at 5283.987 kb on + strand, within Pf6N2E2_4554 at 5283.987 kb on + strand, within Pf6N2E2_4554 at 5283.987 kb on + strand, within Pf6N2E2_4554 at 5283.987 kb on + strand, within Pf6N2E2_4554 at 5283.987 kb on + strand, within Pf6N2E2_4554 at 5283.988 kb on - strand, within Pf6N2E2_4554 at 5283.988 kb on - strand, within Pf6N2E2_4554 at 5283.988 kb on - strand, within Pf6N2E2_4554 at 5283.988 kb on - strand, within Pf6N2E2_4554 at 5283.988 kb on - strand, within Pf6N2E2_4554 at 5283.988 kb on - strand, within Pf6N2E2_4554 at 5283.988 kb on - strand, within Pf6N2E2_4554 at 5283.988 kb on - strand, within Pf6N2E2_4554 at 5283.988 kb on - strand, within Pf6N2E2_4554 at 5283.988 kb on - strand, within Pf6N2E2_4554 at 5283.988 kb on - strand, within Pf6N2E2_4554 at 5283.988 kb on - strand, within Pf6N2E2_4554 at 5283.988 kb on - strand, within Pf6N2E2_4554 at 5283.988 kb on - strand, within Pf6N2E2_4554 at 5283.988 kb on - strand, within Pf6N2E2_4554 at 5283.988 kb on - strand, within Pf6N2E2_4554 at 5283.991 kb on + strand, within Pf6N2E2_4554 at 5284.068 kb on - strand, within Pf6N2E2_4554 at 5284.173 kb on + strand, within Pf6N2E2_4554 at 5284.187 kb on + strand, within Pf6N2E2_4554
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 5,281,668 + Pf6N2E2_4551 0.88 -0.2 5,281,668 + Pf6N2E2_4551 0.88 +1.0 5,281,669 - Pf6N2E2_4551 0.88 +0.0 5,281,669 - Pf6N2E2_4551 0.88 +0.3 5,281,739 + -1.7 5,282,053 + +0.3 5,282,053 + +0.5 5,282,053 + +1.0 5,282,053 + +0.7 5,282,253 + +0.2 5,282,254 - +0.9 5,282,254 - +0.7 5,282,259 + +0.2 5,282,260 - +0.0 5,282,260 - -0.9 5,282,307 + -0.6 5,282,359 + +1.0 5,282,359 + -1.0 5,282,384 + -1.3 5,282,384 + -1.6 5,282,385 - -0.7 5,282,385 - +0.8 5,282,385 - -0.8 5,282,560 + Pf6N2E2_4553 0.23 -0.6 5,282,560 + Pf6N2E2_4553 0.23 -1.5 5,282,560 + Pf6N2E2_4553 0.23 -2.1 5,282,561 - Pf6N2E2_4553 0.23 -0.0 5,282,561 - Pf6N2E2_4553 0.23 -0.9 5,282,654 + Pf6N2E2_4553 0.33 -1.3 5,282,771 + Pf6N2E2_4553 0.46 -1.8 5,282,771 + Pf6N2E2_4553 0.46 -1.1 5,282,954 + Pf6N2E2_4553 0.66 -1.6 5,282,954 + Pf6N2E2_4553 0.66 +0.4 5,282,967 + Pf6N2E2_4553 0.67 -0.3 5,282,968 - Pf6N2E2_4553 0.67 +3.0 5,282,968 - Pf6N2E2_4553 0.67 -0.3 5,283,012 + Pf6N2E2_4553 0.72 -0.6 5,283,233 + -0.4 5,283,233 + -0.2 5,283,233 + +0.8 5,283,376 - -1.0 5,283,376 - +0.2 5,283,378 + -0.9 5,283,379 - -0.5 5,283,404 - +0.8 5,283,416 - -0.2 5,283,461 - +1.0 5,283,583 + -1.6 5,283,583 + -1.2 5,283,583 + -1.0 5,283,583 + +1.4 5,283,601 + +0.8 5,283,614 + -0.5 5,283,614 + -0.6 5,283,614 + +1.1 5,283,614 + +1.2 5,283,614 + +0.7 5,283,615 - -0.5 5,283,615 - -0.1 5,283,655 - +0.0 5,283,664 - -0.5 5,283,667 + -0.3 5,283,730 + +0.5 5,283,731 - +0.3 5,283,811 - Pf6N2E2_4554 0.24 -0.0 5,283,822 + Pf6N2E2_4554 0.26 -0.5 5,283,964 - Pf6N2E2_4554 0.50 +0.3 5,283,983 + Pf6N2E2_4554 0.54 +1.1 5,283,983 + Pf6N2E2_4554 0.54 -0.6 5,283,983 + Pf6N2E2_4554 0.54 -0.4 5,283,983 + Pf6N2E2_4554 0.54 -0.4 5,283,987 + Pf6N2E2_4554 0.54 -1.0 5,283,987 + Pf6N2E2_4554 0.54 +0.7 5,283,987 + Pf6N2E2_4554 0.54 +0.1 5,283,987 + Pf6N2E2_4554 0.54 -0.1 5,283,987 + Pf6N2E2_4554 0.54 +1.9 5,283,987 + Pf6N2E2_4554 0.54 -1.3 5,283,987 + Pf6N2E2_4554 0.54 -2.5 5,283,987 + Pf6N2E2_4554 0.54 +0.9 5,283,987 + Pf6N2E2_4554 0.54 +0.4 5,283,987 + Pf6N2E2_4554 0.54 -0.2 5,283,987 + Pf6N2E2_4554 0.54 +0.8 5,283,987 + Pf6N2E2_4554 0.54 +0.6 5,283,987 + Pf6N2E2_4554 0.54 +0.0 5,283,987 + Pf6N2E2_4554 0.54 +1.0 5,283,987 + Pf6N2E2_4554 0.54 +0.3 5,283,988 - Pf6N2E2_4554 0.55 -0.4 5,283,988 - Pf6N2E2_4554 0.55 +0.3 5,283,988 - Pf6N2E2_4554 0.55 -0.4 5,283,988 - Pf6N2E2_4554 0.55 -1.3 5,283,988 - Pf6N2E2_4554 0.55 -0.9 5,283,988 - Pf6N2E2_4554 0.55 +0.7 5,283,988 - Pf6N2E2_4554 0.55 -0.4 5,283,988 - Pf6N2E2_4554 0.55 -0.8 5,283,988 - Pf6N2E2_4554 0.55 -0.1 5,283,988 - Pf6N2E2_4554 0.55 -0.4 5,283,988 - Pf6N2E2_4554 0.55 +2.0 5,283,988 - Pf6N2E2_4554 0.55 +1.0 5,283,988 - Pf6N2E2_4554 0.55 -0.4 5,283,988 - Pf6N2E2_4554 0.55 -0.4 5,283,988 - Pf6N2E2_4554 0.55 -0.2 5,283,988 - Pf6N2E2_4554 0.55 -0.8 5,283,991 + Pf6N2E2_4554 0.55 +0.1 5,284,068 - Pf6N2E2_4554 0.68 +0.8 5,284,173 + Pf6N2E2_4554 0.87 -0.1 5,284,187 + Pf6N2E2_4554 0.89 +0.0
Or see this region's nucleotide sequence