Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3619 and Pf6N2E2_3620 are separated by 63 nucleotides Pf6N2E2_3620 and Pf6N2E2_3621 overlap by 19 nucleotides Pf6N2E2_3621 and Pf6N2E2_3622 are separated by 264 nucleotides
Pf6N2E2_3619 - NAD(P)HX epimerase / NAD(P)HX dehydratase, at 4,214,277 to 4,215,776
_3619
Pf6N2E2_3620 - Epoxyqueuosine (oQ) reductase QueG, at 4,215,840 to 4,216,919
_3620
Pf6N2E2_3621 - putative transporter, required for glycine utilization (from data) , at 4,216,901 to 4,217,512
_3621
Pf6N2E2_3622 - 3'-to-5' oligoribonuclease (orn), at 4,217,777 to 4,218,319
_3622
Position (kb)
4215
4216
4217 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4214.916 kb on + strand, within Pf6N2E2_3619 at 4214.916 kb on + strand, within Pf6N2E2_3619 at 4215.087 kb on - strand, within Pf6N2E2_3619 at 4215.132 kb on - strand, within Pf6N2E2_3619 at 4215.132 kb on - strand, within Pf6N2E2_3619 at 4215.132 kb on - strand, within Pf6N2E2_3619 at 4215.132 kb on - strand, within Pf6N2E2_3619 at 4215.132 kb on - strand, within Pf6N2E2_3619 at 4215.132 kb on - strand, within Pf6N2E2_3619 at 4215.350 kb on - strand, within Pf6N2E2_3619 at 4215.350 kb on - strand, within Pf6N2E2_3619 at 4215.350 kb on - strand, within Pf6N2E2_3619 at 4215.350 kb on - strand, within Pf6N2E2_3619 at 4215.350 kb on - strand, within Pf6N2E2_3619 at 4215.350 kb on - strand, within Pf6N2E2_3619 at 4215.350 kb on - strand, within Pf6N2E2_3619 at 4215.350 kb on - strand, within Pf6N2E2_3619 at 4215.740 kb on - strand at 4215.740 kb on - strand at 4215.740 kb on - strand at 4215.740 kb on - strand at 4215.765 kb on - strand at 4215.765 kb on - strand at 4215.765 kb on - strand at 4215.808 kb on - strand at 4215.808 kb on - strand at 4215.808 kb on - strand at 4215.809 kb on + strand at 4215.809 kb on + strand at 4215.809 kb on + strand at 4215.810 kb on - strand at 4215.810 kb on - strand at 4215.810 kb on - strand at 4215.810 kb on - strand at 4215.810 kb on - strand at 4216.002 kb on + strand, within Pf6N2E2_3620 at 4216.002 kb on + strand, within Pf6N2E2_3620 at 4216.002 kb on + strand, within Pf6N2E2_3620 at 4216.003 kb on - strand, within Pf6N2E2_3620 at 4216.003 kb on - strand, within Pf6N2E2_3620 at 4216.003 kb on - strand, within Pf6N2E2_3620 at 4216.003 kb on - strand, within Pf6N2E2_3620 at 4216.087 kb on - strand, within Pf6N2E2_3620 at 4216.087 kb on - strand, within Pf6N2E2_3620 at 4216.087 kb on - strand, within Pf6N2E2_3620 at 4216.140 kb on + strand, within Pf6N2E2_3620 at 4216.140 kb on + strand, within Pf6N2E2_3620 at 4216.140 kb on + strand, within Pf6N2E2_3620 at 4216.141 kb on - strand, within Pf6N2E2_3620 at 4216.141 kb on - strand, within Pf6N2E2_3620 at 4216.141 kb on - strand, within Pf6N2E2_3620 at 4216.152 kb on + strand, within Pf6N2E2_3620 at 4216.170 kb on + strand, within Pf6N2E2_3620 at 4216.317 kb on - strand, within Pf6N2E2_3620 at 4216.353 kb on + strand, within Pf6N2E2_3620 at 4216.353 kb on + strand, within Pf6N2E2_3620 at 4216.353 kb on + strand, within Pf6N2E2_3620 at 4216.354 kb on - strand, within Pf6N2E2_3620 at 4216.457 kb on + strand, within Pf6N2E2_3620 at 4216.457 kb on + strand, within Pf6N2E2_3620 at 4216.457 kb on + strand, within Pf6N2E2_3620 at 4216.458 kb on - strand, within Pf6N2E2_3620 at 4216.479 kb on + strand, within Pf6N2E2_3620 at 4216.479 kb on + strand, within Pf6N2E2_3620 at 4216.480 kb on - strand, within Pf6N2E2_3620 at 4216.988 kb on - strand, within Pf6N2E2_3621 at 4216.988 kb on - strand, within Pf6N2E2_3621 at 4216.988 kb on - strand, within Pf6N2E2_3621 at 4217.037 kb on + strand, within Pf6N2E2_3621 at 4217.037 kb on + strand, within Pf6N2E2_3621 at 4217.037 kb on + strand, within Pf6N2E2_3621 at 4217.037 kb on + strand, within Pf6N2E2_3621 at 4217.037 kb on + strand, within Pf6N2E2_3621 at 4217.037 kb on + strand, within Pf6N2E2_3621 at 4217.037 kb on + strand, within Pf6N2E2_3621 at 4217.070 kb on + strand, within Pf6N2E2_3621 at 4217.070 kb on + strand, within Pf6N2E2_3621 at 4217.070 kb on + strand, within Pf6N2E2_3621 at 4217.071 kb on - strand, within Pf6N2E2_3621 at 4217.071 kb on - strand, within Pf6N2E2_3621 at 4217.071 kb on - strand, within Pf6N2E2_3621 at 4217.099 kb on - strand, within Pf6N2E2_3621 at 4217.099 kb on - strand, within Pf6N2E2_3621 at 4217.321 kb on + strand, within Pf6N2E2_3621 at 4217.321 kb on + strand, within Pf6N2E2_3621 at 4217.322 kb on - strand, within Pf6N2E2_3621 at 4217.322 kb on - strand, within Pf6N2E2_3621 at 4217.322 kb on - strand, within Pf6N2E2_3621 at 4217.322 kb on - strand, within Pf6N2E2_3621 at 4217.326 kb on - strand, within Pf6N2E2_3621 at 4217.326 kb on - strand, within Pf6N2E2_3621 at 4217.496 kb on + strand at 4217.496 kb on + strand at 4217.496 kb on + strand at 4217.497 kb on - strand at 4217.598 kb on + strand at 4217.598 kb on + strand at 4217.598 kb on + strand at 4217.599 kb on - strand at 4217.599 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 4,214,916 + Pf6N2E2_3619 0.43 -1.0 4,214,916 + Pf6N2E2_3619 0.43 -0.9 4,215,087 - Pf6N2E2_3619 0.54 -1.7 4,215,132 - Pf6N2E2_3619 0.57 +0.2 4,215,132 - Pf6N2E2_3619 0.57 -3.0 4,215,132 - Pf6N2E2_3619 0.57 -0.5 4,215,132 - Pf6N2E2_3619 0.57 +0.0 4,215,132 - Pf6N2E2_3619 0.57 -0.3 4,215,132 - Pf6N2E2_3619 0.57 -1.6 4,215,350 - Pf6N2E2_3619 0.72 +0.3 4,215,350 - Pf6N2E2_3619 0.72 +3.3 4,215,350 - Pf6N2E2_3619 0.72 -1.1 4,215,350 - Pf6N2E2_3619 0.72 +0.1 4,215,350 - Pf6N2E2_3619 0.72 +0.9 4,215,350 - Pf6N2E2_3619 0.72 +0.4 4,215,350 - Pf6N2E2_3619 0.72 -0.8 4,215,350 - Pf6N2E2_3619 0.72 +0.4 4,215,740 - +1.0 4,215,740 - -0.7 4,215,740 - -0.9 4,215,740 - +0.7 4,215,765 - -0.3 4,215,765 - -0.1 4,215,765 - +0.1 4,215,808 - +1.0 4,215,808 - -1.3 4,215,808 - -0.6 4,215,809 + +0.2 4,215,809 + +0.5 4,215,809 + +0.7 4,215,810 - -0.6 4,215,810 - +0.7 4,215,810 - -0.9 4,215,810 - +0.8 4,215,810 - +0.3 4,216,002 + Pf6N2E2_3620 0.15 -0.3 4,216,002 + Pf6N2E2_3620 0.15 -1.1 4,216,002 + Pf6N2E2_3620 0.15 +0.7 4,216,003 - Pf6N2E2_3620 0.15 -0.2 4,216,003 - Pf6N2E2_3620 0.15 -1.3 4,216,003 - Pf6N2E2_3620 0.15 -0.8 4,216,003 - Pf6N2E2_3620 0.15 -0.7 4,216,087 - Pf6N2E2_3620 0.23 +0.1 4,216,087 - Pf6N2E2_3620 0.23 -1.5 4,216,087 - Pf6N2E2_3620 0.23 -1.2 4,216,140 + Pf6N2E2_3620 0.28 -2.5 4,216,140 + Pf6N2E2_3620 0.28 -1.8 4,216,140 + Pf6N2E2_3620 0.28 -1.0 4,216,141 - Pf6N2E2_3620 0.28 -0.1 4,216,141 - Pf6N2E2_3620 0.28 -0.9 4,216,141 - Pf6N2E2_3620 0.28 -2.5 4,216,152 + Pf6N2E2_3620 0.29 -2.0 4,216,170 + Pf6N2E2_3620 0.31 +0.0 4,216,317 - Pf6N2E2_3620 0.44 -0.7 4,216,353 + Pf6N2E2_3620 0.47 -0.1 4,216,353 + Pf6N2E2_3620 0.47 -1.8 4,216,353 + Pf6N2E2_3620 0.47 +0.4 4,216,354 - Pf6N2E2_3620 0.48 -2.1 4,216,457 + Pf6N2E2_3620 0.57 +0.2 4,216,457 + Pf6N2E2_3620 0.57 +0.4 4,216,457 + Pf6N2E2_3620 0.57 -0.8 4,216,458 - Pf6N2E2_3620 0.57 -0.2 4,216,479 + Pf6N2E2_3620 0.59 -0.9 4,216,479 + Pf6N2E2_3620 0.59 +0.9 4,216,480 - Pf6N2E2_3620 0.59 -2.5 4,216,988 - Pf6N2E2_3621 0.14 -0.5 4,216,988 - Pf6N2E2_3621 0.14 +0.3 4,216,988 - Pf6N2E2_3621 0.14 +0.4 4,217,037 + Pf6N2E2_3621 0.22 +0.2 4,217,037 + Pf6N2E2_3621 0.22 -1.0 4,217,037 + Pf6N2E2_3621 0.22 -0.2 4,217,037 + Pf6N2E2_3621 0.22 +0.7 4,217,037 + Pf6N2E2_3621 0.22 +1.6 4,217,037 + Pf6N2E2_3621 0.22 -1.1 4,217,037 + Pf6N2E2_3621 0.22 +0.3 4,217,070 + Pf6N2E2_3621 0.28 +0.1 4,217,070 + Pf6N2E2_3621 0.28 -0.8 4,217,070 + Pf6N2E2_3621 0.28 +0.0 4,217,071 - Pf6N2E2_3621 0.28 +0.3 4,217,071 - Pf6N2E2_3621 0.28 -0.4 4,217,071 - Pf6N2E2_3621 0.28 +0.3 4,217,099 - Pf6N2E2_3621 0.32 -0.4 4,217,099 - Pf6N2E2_3621 0.32 -0.7 4,217,321 + Pf6N2E2_3621 0.69 +1.6 4,217,321 + Pf6N2E2_3621 0.69 +1.0 4,217,322 - Pf6N2E2_3621 0.69 -0.5 4,217,322 - Pf6N2E2_3621 0.69 -0.9 4,217,322 - Pf6N2E2_3621 0.69 +1.4 4,217,322 - Pf6N2E2_3621 0.69 -2.1 4,217,326 - Pf6N2E2_3621 0.69 -0.9 4,217,326 - Pf6N2E2_3621 0.69 +0.4 4,217,496 + -1.0 4,217,496 + -0.2 4,217,496 + +1.6 4,217,497 - +0.7 4,217,598 + -0.2 4,217,598 + -2.6 4,217,598 + +0.7 4,217,599 - -0.1 4,217,599 - -0.3
Or see this region's nucleotide sequence