Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1977 and Pf6N2E2_1978 are separated by 8 nucleotides Pf6N2E2_1978 and Pf6N2E2_1979 are separated by 461 nucleotides
Pf6N2E2_1977 - DNA topoisomerase III (EC 5.99.1.2) in PFGI-1-like cluster, at 2,342,473 to 2,344,023
_1977
Pf6N2E2_1978 - DNA topoisomerase III (EC 5.99.1.2) in PFGI-1-like cluster, at 2,344,032 to 2,344,559
_1978
Pf6N2E2_1979 - Cytosine-specific DNA methyltransferase (EC 2.1.1.37), at 2,345,021 to 2,346,277
_1979
Position (kb)
2344
2345 Strain fitness (log2 ratio)
-2
-1
0
1 at 2343.090 kb on + strand, within Pf6N2E2_1977 at 2343.131 kb on + strand, within Pf6N2E2_1977 at 2343.131 kb on + strand, within Pf6N2E2_1977 at 2343.132 kb on - strand, within Pf6N2E2_1977 at 2343.138 kb on - strand, within Pf6N2E2_1977 at 2343.212 kb on + strand, within Pf6N2E2_1977 at 2343.212 kb on + strand, within Pf6N2E2_1977 at 2343.342 kb on - strand, within Pf6N2E2_1977 at 2343.472 kb on + strand, within Pf6N2E2_1977 at 2343.727 kb on + strand, within Pf6N2E2_1977 at 2343.727 kb on + strand, within Pf6N2E2_1977 at 2343.748 kb on + strand, within Pf6N2E2_1977 at 2343.779 kb on - strand, within Pf6N2E2_1977 at 2343.818 kb on + strand, within Pf6N2E2_1977 at 2343.819 kb on - strand, within Pf6N2E2_1977 at 2343.819 kb on - strand, within Pf6N2E2_1977 at 2343.972 kb on + strand at 2343.973 kb on - strand at 2344.005 kb on + strand at 2344.124 kb on + strand, within Pf6N2E2_1978 at 2344.152 kb on + strand, within Pf6N2E2_1978 at 2344.153 kb on - strand, within Pf6N2E2_1978 at 2344.153 kb on - strand, within Pf6N2E2_1978 at 2344.158 kb on + strand, within Pf6N2E2_1978 at 2344.159 kb on - strand, within Pf6N2E2_1978 at 2344.159 kb on - strand, within Pf6N2E2_1978 at 2344.159 kb on - strand, within Pf6N2E2_1978 at 2344.159 kb on - strand, within Pf6N2E2_1978 at 2344.159 kb on - strand, within Pf6N2E2_1978 at 2344.659 kb on - strand at 2344.659 kb on - strand at 2344.749 kb on + strand at 2344.749 kb on + strand at 2344.749 kb on + strand at 2344.750 kb on - strand at 2344.750 kb on - strand at 2344.750 kb on - strand at 2344.750 kb on - strand at 2345.048 kb on + strand at 2345.101 kb on - strand at 2345.130 kb on + strand at 2345.131 kb on - strand at 2345.175 kb on + strand, within Pf6N2E2_1979 at 2345.176 kb on - strand, within Pf6N2E2_1979 at 2345.180 kb on - strand, within Pf6N2E2_1979 at 2345.180 kb on - strand, within Pf6N2E2_1979
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 2,343,090 + Pf6N2E2_1977 0.40 -0.3 2,343,131 + Pf6N2E2_1977 0.42 +0.9 2,343,131 + Pf6N2E2_1977 0.42 +1.2 2,343,132 - Pf6N2E2_1977 0.42 -0.0 2,343,138 - Pf6N2E2_1977 0.43 +0.5 2,343,212 + Pf6N2E2_1977 0.48 +0.8 2,343,212 + Pf6N2E2_1977 0.48 -1.1 2,343,342 - Pf6N2E2_1977 0.56 +0.0 2,343,472 + Pf6N2E2_1977 0.64 +0.8 2,343,727 + Pf6N2E2_1977 0.81 -0.5 2,343,727 + Pf6N2E2_1977 0.81 -1.2 2,343,748 + Pf6N2E2_1977 0.82 -1.1 2,343,779 - Pf6N2E2_1977 0.84 -0.1 2,343,818 + Pf6N2E2_1977 0.87 +0.5 2,343,819 - Pf6N2E2_1977 0.87 -0.8 2,343,819 - Pf6N2E2_1977 0.87 +0.2 2,343,972 + -0.3 2,343,973 - -0.4 2,344,005 + +0.1 2,344,124 + Pf6N2E2_1978 0.17 +0.5 2,344,152 + Pf6N2E2_1978 0.23 +1.2 2,344,153 - Pf6N2E2_1978 0.23 +1.3 2,344,153 - Pf6N2E2_1978 0.23 +0.6 2,344,158 + Pf6N2E2_1978 0.24 +0.5 2,344,159 - Pf6N2E2_1978 0.24 +0.5 2,344,159 - Pf6N2E2_1978 0.24 +0.8 2,344,159 - Pf6N2E2_1978 0.24 +1.5 2,344,159 - Pf6N2E2_1978 0.24 +0.5 2,344,159 - Pf6N2E2_1978 0.24 -2.0 2,344,659 - -0.4 2,344,659 - -0.9 2,344,749 + -0.9 2,344,749 + -1.3 2,344,749 + +0.8 2,344,750 - -0.5 2,344,750 - +0.0 2,344,750 - +0.2 2,344,750 - +1.1 2,345,048 + -0.5 2,345,101 - -0.5 2,345,130 + +0.6 2,345,131 - +0.4 2,345,175 + Pf6N2E2_1979 0.12 +0.5 2,345,176 - Pf6N2E2_1979 0.12 -0.7 2,345,180 - Pf6N2E2_1979 0.13 +0.5 2,345,180 - Pf6N2E2_1979 0.13 +1.1
Or see this region's nucleotide sequence