Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1978

Experiment: LB no stress 48 hours

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1977 and Pf6N2E2_1978 are separated by 8 nucleotidesPf6N2E2_1978 and Pf6N2E2_1979 are separated by 461 nucleotides Pf6N2E2_1977 - DNA topoisomerase III (EC 5.99.1.2) in PFGI-1-like cluster, at 2,342,473 to 2,344,023 _1977 Pf6N2E2_1978 - DNA topoisomerase III (EC 5.99.1.2) in PFGI-1-like cluster, at 2,344,032 to 2,344,559 _1978 Pf6N2E2_1979 - Cytosine-specific DNA methyltransferase (EC 2.1.1.37), at 2,345,021 to 2,346,277 _1979 Position (kb) 2344 2345Strain fitness (log2 ratio) -2 -1 0 1at 2343.090 kb on + strand, within Pf6N2E2_1977at 2343.131 kb on + strand, within Pf6N2E2_1977at 2343.131 kb on + strand, within Pf6N2E2_1977at 2343.132 kb on - strand, within Pf6N2E2_1977at 2343.138 kb on - strand, within Pf6N2E2_1977at 2343.212 kb on + strand, within Pf6N2E2_1977at 2343.212 kb on + strand, within Pf6N2E2_1977at 2343.342 kb on - strand, within Pf6N2E2_1977at 2343.472 kb on + strand, within Pf6N2E2_1977at 2343.727 kb on + strand, within Pf6N2E2_1977at 2343.727 kb on + strand, within Pf6N2E2_1977at 2343.748 kb on + strand, within Pf6N2E2_1977at 2343.779 kb on - strand, within Pf6N2E2_1977at 2343.818 kb on + strand, within Pf6N2E2_1977at 2343.819 kb on - strand, within Pf6N2E2_1977at 2343.819 kb on - strand, within Pf6N2E2_1977at 2343.972 kb on + strandat 2343.973 kb on - strandat 2344.005 kb on + strandat 2344.124 kb on + strand, within Pf6N2E2_1978at 2344.152 kb on + strand, within Pf6N2E2_1978at 2344.153 kb on - strand, within Pf6N2E2_1978at 2344.153 kb on - strand, within Pf6N2E2_1978at 2344.158 kb on + strand, within Pf6N2E2_1978at 2344.159 kb on - strand, within Pf6N2E2_1978at 2344.159 kb on - strand, within Pf6N2E2_1978at 2344.159 kb on - strand, within Pf6N2E2_1978at 2344.159 kb on - strand, within Pf6N2E2_1978at 2344.159 kb on - strand, within Pf6N2E2_1978at 2344.659 kb on - strandat 2344.659 kb on - strandat 2344.749 kb on + strandat 2344.749 kb on + strandat 2344.749 kb on + strandat 2344.750 kb on - strandat 2344.750 kb on - strandat 2344.750 kb on - strandat 2344.750 kb on - strandat 2345.048 kb on + strandat 2345.101 kb on - strandat 2345.130 kb on + strandat 2345.131 kb on - strandat 2345.175 kb on + strand, within Pf6N2E2_1979at 2345.176 kb on - strand, within Pf6N2E2_1979at 2345.180 kb on - strand, within Pf6N2E2_1979at 2345.180 kb on - strand, within Pf6N2E2_1979

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Per-strain Table

Position Strand Gene LocusTag Fraction LB no stress 48 hours
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2,343,090 + Pf6N2E2_1977 0.40 -0.3
2,343,131 + Pf6N2E2_1977 0.42 +0.9
2,343,131 + Pf6N2E2_1977 0.42 +1.2
2,343,132 - Pf6N2E2_1977 0.42 -0.0
2,343,138 - Pf6N2E2_1977 0.43 +0.5
2,343,212 + Pf6N2E2_1977 0.48 +0.8
2,343,212 + Pf6N2E2_1977 0.48 -1.1
2,343,342 - Pf6N2E2_1977 0.56 +0.0
2,343,472 + Pf6N2E2_1977 0.64 +0.8
2,343,727 + Pf6N2E2_1977 0.81 -0.5
2,343,727 + Pf6N2E2_1977 0.81 -1.2
2,343,748 + Pf6N2E2_1977 0.82 -1.1
2,343,779 - Pf6N2E2_1977 0.84 -0.1
2,343,818 + Pf6N2E2_1977 0.87 +0.5
2,343,819 - Pf6N2E2_1977 0.87 -0.8
2,343,819 - Pf6N2E2_1977 0.87 +0.2
2,343,972 + -0.3
2,343,973 - -0.4
2,344,005 + +0.1
2,344,124 + Pf6N2E2_1978 0.17 +0.5
2,344,152 + Pf6N2E2_1978 0.23 +1.2
2,344,153 - Pf6N2E2_1978 0.23 +1.3
2,344,153 - Pf6N2E2_1978 0.23 +0.6
2,344,158 + Pf6N2E2_1978 0.24 +0.5
2,344,159 - Pf6N2E2_1978 0.24 +0.5
2,344,159 - Pf6N2E2_1978 0.24 +0.8
2,344,159 - Pf6N2E2_1978 0.24 +1.5
2,344,159 - Pf6N2E2_1978 0.24 +0.5
2,344,159 - Pf6N2E2_1978 0.24 -2.0
2,344,659 - -0.4
2,344,659 - -0.9
2,344,749 + -0.9
2,344,749 + -1.3
2,344,749 + +0.8
2,344,750 - -0.5
2,344,750 - +0.0
2,344,750 - +0.2
2,344,750 - +1.1
2,345,048 + -0.5
2,345,101 - -0.5
2,345,130 + +0.6
2,345,131 - +0.4
2,345,175 + Pf6N2E2_1979 0.12 +0.5
2,345,176 - Pf6N2E2_1979 0.12 -0.7
2,345,180 - Pf6N2E2_1979 0.13 +0.5
2,345,180 - Pf6N2E2_1979 0.13 +1.1

Or see this region's nucleotide sequence