Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1861 and Pf6N2E2_1862 are separated by 334 nucleotides Pf6N2E2_1862 and Pf6N2E2_1863 are separated by 0 nucleotides
Pf6N2E2_1861 - Ferredoxin reductase, at 2,204,360 to 2,205,595
_1861
Pf6N2E2_1862 - Benzoate 1,2-dioxygenase beta subunit (EC 1.14.12.10), at 2,205,930 to 2,206,424
_1862
Pf6N2E2_1863 - putative dioxygenase hydroxylase component, at 2,206,425 to 2,207,684
_1863
Position (kb)
2205
2206
2207 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2205.013 kb on - strand, within Pf6N2E2_1861 at 2205.013 kb on - strand, within Pf6N2E2_1861 at 2205.207 kb on + strand, within Pf6N2E2_1861 at 2205.207 kb on + strand, within Pf6N2E2_1861 at 2205.285 kb on + strand, within Pf6N2E2_1861 at 2205.438 kb on + strand, within Pf6N2E2_1861 at 2205.439 kb on - strand, within Pf6N2E2_1861 at 2205.591 kb on + strand at 2205.592 kb on - strand at 2205.619 kb on + strand at 2205.620 kb on - strand at 2205.701 kb on + strand at 2205.701 kb on + strand at 2205.701 kb on + strand at 2205.702 kb on - strand at 2206.031 kb on - strand, within Pf6N2E2_1862 at 2206.092 kb on - strand, within Pf6N2E2_1862 at 2206.092 kb on - strand, within Pf6N2E2_1862 at 2206.194 kb on - strand, within Pf6N2E2_1862 at 2206.237 kb on + strand, within Pf6N2E2_1862 at 2206.237 kb on + strand, within Pf6N2E2_1862 at 2206.241 kb on + strand, within Pf6N2E2_1862 at 2206.295 kb on - strand, within Pf6N2E2_1862 at 2206.343 kb on + strand, within Pf6N2E2_1862 at 2206.427 kb on - strand at 2206.550 kb on - strand at 2206.597 kb on + strand, within Pf6N2E2_1863 at 2206.840 kb on + strand, within Pf6N2E2_1863 at 2206.841 kb on - strand, within Pf6N2E2_1863 at 2206.865 kb on - strand, within Pf6N2E2_1863 at 2206.996 kb on + strand, within Pf6N2E2_1863 at 2206.996 kb on + strand, within Pf6N2E2_1863 at 2206.997 kb on - strand, within Pf6N2E2_1863 at 2206.997 kb on - strand, within Pf6N2E2_1863 at 2206.997 kb on - strand, within Pf6N2E2_1863 at 2206.997 kb on - strand, within Pf6N2E2_1863 at 2206.997 kb on - strand, within Pf6N2E2_1863 at 2207.251 kb on + strand, within Pf6N2E2_1863 at 2207.369 kb on - strand, within Pf6N2E2_1863 at 2207.379 kb on - strand, within Pf6N2E2_1863 at 2207.379 kb on - strand, within Pf6N2E2_1863
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 2,205,013 - Pf6N2E2_1861 0.53 +0.6 2,205,013 - Pf6N2E2_1861 0.53 +0.7 2,205,207 + Pf6N2E2_1861 0.69 -1.9 2,205,207 + Pf6N2E2_1861 0.69 +0.6 2,205,285 + Pf6N2E2_1861 0.75 +1.8 2,205,438 + Pf6N2E2_1861 0.87 -1.1 2,205,439 - Pf6N2E2_1861 0.87 -0.9 2,205,591 + -0.2 2,205,592 - +1.4 2,205,619 + -1.2 2,205,620 - +0.2 2,205,701 + +1.8 2,205,701 + +1.5 2,205,701 + -1.5 2,205,702 - +0.4 2,206,031 - Pf6N2E2_1862 0.20 +1.4 2,206,092 - Pf6N2E2_1862 0.33 +3.0 2,206,092 - Pf6N2E2_1862 0.33 +1.4 2,206,194 - Pf6N2E2_1862 0.53 -0.6 2,206,237 + Pf6N2E2_1862 0.62 +0.2 2,206,237 + Pf6N2E2_1862 0.62 -0.0 2,206,241 + Pf6N2E2_1862 0.63 +1.1 2,206,295 - Pf6N2E2_1862 0.74 +0.5 2,206,343 + Pf6N2E2_1862 0.83 -1.8 2,206,427 - +0.2 2,206,550 - -0.8 2,206,597 + Pf6N2E2_1863 0.14 +0.1 2,206,840 + Pf6N2E2_1863 0.33 +0.3 2,206,841 - Pf6N2E2_1863 0.33 -1.3 2,206,865 - Pf6N2E2_1863 0.35 +0.8 2,206,996 + Pf6N2E2_1863 0.45 +0.7 2,206,996 + Pf6N2E2_1863 0.45 +0.1 2,206,997 - Pf6N2E2_1863 0.45 -0.5 2,206,997 - Pf6N2E2_1863 0.45 +2.4 2,206,997 - Pf6N2E2_1863 0.45 -0.5 2,206,997 - Pf6N2E2_1863 0.45 +0.8 2,206,997 - Pf6N2E2_1863 0.45 -0.4 2,207,251 + Pf6N2E2_1863 0.66 -0.4 2,207,369 - Pf6N2E2_1863 0.75 -0.5 2,207,379 - Pf6N2E2_1863 0.76 -3.0 2,207,379 - Pf6N2E2_1863 0.76 -1.8
Or see this region's nucleotide sequence