Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1578 and Pf6N2E2_1579 are separated by 465 nucleotides Pf6N2E2_1579 and Pf6N2E2_1580 are separated by 84 nucleotides
Pf6N2E2_1578 - T1SS secreted agglutinin RTX, at 1,843,752 to 1,847,927
_1578
Pf6N2E2_1579 - hypothetical protein, at 1,848,393 to 1,848,752
_1579
Pf6N2E2_1580 - putative two-component system response regulator, at 1,848,837 to 1,849,454
_1580
Position (kb)
1848
1849 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1847.724 kb on + strand at 1847.747 kb on - strand at 1847.873 kb on + strand at 1847.873 kb on + strand at 1847.873 kb on + strand at 1847.873 kb on + strand at 1847.873 kb on + strand at 1847.874 kb on - strand at 1847.988 kb on + strand at 1848.057 kb on - strand at 1848.089 kb on - strand at 1848.089 kb on - strand at 1848.110 kb on + strand at 1848.110 kb on + strand at 1848.110 kb on + strand at 1848.116 kb on + strand at 1848.117 kb on - strand at 1848.117 kb on - strand at 1848.117 kb on - strand at 1848.158 kb on + strand at 1848.212 kb on - strand at 1848.219 kb on + strand at 1848.219 kb on + strand at 1848.219 kb on + strand at 1848.220 kb on - strand at 1848.220 kb on - strand at 1848.220 kb on - strand at 1848.256 kb on + strand at 1848.256 kb on + strand at 1848.257 kb on - strand at 1848.257 kb on - strand at 1848.272 kb on + strand at 1848.272 kb on + strand at 1848.272 kb on + strand at 1848.273 kb on - strand at 1848.273 kb on - strand at 1848.273 kb on - strand at 1848.273 kb on - strand at 1848.273 kb on - strand at 1848.273 kb on - strand at 1848.273 kb on - strand at 1848.276 kb on + strand at 1848.276 kb on + strand at 1848.277 kb on - strand at 1848.277 kb on - strand at 1848.277 kb on - strand at 1848.304 kb on + strand at 1848.304 kb on + strand at 1848.304 kb on + strand at 1848.304 kb on + strand at 1848.305 kb on - strand at 1848.305 kb on - strand at 1848.380 kb on + strand at 1848.381 kb on - strand at 1848.441 kb on + strand, within Pf6N2E2_1579 at 1848.441 kb on + strand, within Pf6N2E2_1579 at 1848.441 kb on + strand, within Pf6N2E2_1579 at 1848.441 kb on + strand, within Pf6N2E2_1579 at 1848.495 kb on + strand, within Pf6N2E2_1579 at 1848.499 kb on - strand, within Pf6N2E2_1579 at 1848.499 kb on - strand, within Pf6N2E2_1579 at 1848.499 kb on - strand, within Pf6N2E2_1579 at 1848.791 kb on - strand at 1848.791 kb on - strand at 1848.804 kb on - strand at 1848.904 kb on + strand, within Pf6N2E2_1580 at 1848.904 kb on + strand, within Pf6N2E2_1580 at 1848.904 kb on + strand, within Pf6N2E2_1580 at 1848.905 kb on - strand, within Pf6N2E2_1580 at 1848.905 kb on - strand, within Pf6N2E2_1580 at 1848.905 kb on - strand, within Pf6N2E2_1580 at 1848.905 kb on - strand, within Pf6N2E2_1580 at 1848.905 kb on - strand, within Pf6N2E2_1580 at 1848.905 kb on - strand, within Pf6N2E2_1580 at 1848.946 kb on + strand, within Pf6N2E2_1580 at 1848.946 kb on + strand at 1848.947 kb on - strand, within Pf6N2E2_1580 at 1848.953 kb on + strand, within Pf6N2E2_1580 at 1848.954 kb on - strand, within Pf6N2E2_1580 at 1848.954 kb on - strand, within Pf6N2E2_1580 at 1849.012 kb on + strand, within Pf6N2E2_1580 at 1849.012 kb on + strand, within Pf6N2E2_1580 at 1849.012 kb on + strand, within Pf6N2E2_1580 at 1849.013 kb on - strand, within Pf6N2E2_1580 at 1849.013 kb on - strand, within Pf6N2E2_1580 at 1849.282 kb on + strand, within Pf6N2E2_1580 at 1849.424 kb on + strand at 1849.426 kb on + strand at 1849.426 kb on + strand at 1849.426 kb on + strand at 1849.541 kb on + strand at 1849.541 kb on + strand at 1849.682 kb on - strand at 1849.691 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 1,847,724 + +0.7 1,847,747 - -1.1 1,847,873 + -0.4 1,847,873 + +0.2 1,847,873 + -0.5 1,847,873 + +0.1 1,847,873 + -0.6 1,847,874 - +0.9 1,847,988 + -0.3 1,848,057 - +0.3 1,848,089 - -2.0 1,848,089 - +0.9 1,848,110 + +0.8 1,848,110 + -0.4 1,848,110 + -0.6 1,848,116 + +1.3 1,848,117 - -0.1 1,848,117 - +0.0 1,848,117 - -0.6 1,848,158 + -1.5 1,848,212 - +0.8 1,848,219 + -0.6 1,848,219 + +0.4 1,848,219 + -0.8 1,848,220 - -1.6 1,848,220 - +1.9 1,848,220 - +0.5 1,848,256 + -0.5 1,848,256 + +1.4 1,848,257 - +0.8 1,848,257 - +0.8 1,848,272 + -1.8 1,848,272 + +0.6 1,848,272 + +0.1 1,848,273 - -0.2 1,848,273 - -0.1 1,848,273 - -0.6 1,848,273 - -0.2 1,848,273 - +0.2 1,848,273 - +0.4 1,848,273 - +2.7 1,848,276 + +0.0 1,848,276 + +0.9 1,848,277 - -1.1 1,848,277 - -0.1 1,848,277 - -2.2 1,848,304 + -1.1 1,848,304 + +0.9 1,848,304 + -0.5 1,848,304 + -0.3 1,848,305 - -1.2 1,848,305 - -1.9 1,848,380 + +2.4 1,848,381 - +1.6 1,848,441 + Pf6N2E2_1579 0.13 +0.4 1,848,441 + Pf6N2E2_1579 0.13 +0.2 1,848,441 + Pf6N2E2_1579 0.13 +0.5 1,848,441 + Pf6N2E2_1579 0.13 +0.2 1,848,495 + Pf6N2E2_1579 0.28 -1.1 1,848,499 - Pf6N2E2_1579 0.29 +2.1 1,848,499 - Pf6N2E2_1579 0.29 +1.4 1,848,499 - Pf6N2E2_1579 0.29 +0.7 1,848,791 - -0.6 1,848,791 - -0.5 1,848,804 - -0.8 1,848,904 + Pf6N2E2_1580 0.11 +0.6 1,848,904 + Pf6N2E2_1580 0.11 +0.6 1,848,904 + Pf6N2E2_1580 0.11 -0.5 1,848,905 - Pf6N2E2_1580 0.11 -0.9 1,848,905 - Pf6N2E2_1580 0.11 +1.4 1,848,905 - Pf6N2E2_1580 0.11 +0.0 1,848,905 - Pf6N2E2_1580 0.11 +0.7 1,848,905 - Pf6N2E2_1580 0.11 -0.5 1,848,905 - Pf6N2E2_1580 0.11 +0.0 1,848,946 + Pf6N2E2_1580 0.18 +1.4 1,848,946 + +0.4 1,848,947 - Pf6N2E2_1580 0.18 +2.7 1,848,953 + Pf6N2E2_1580 0.19 -1.2 1,848,954 - Pf6N2E2_1580 0.19 -0.6 1,848,954 - Pf6N2E2_1580 0.19 -0.2 1,849,012 + Pf6N2E2_1580 0.28 -1.9 1,849,012 + Pf6N2E2_1580 0.28 +0.4 1,849,012 + Pf6N2E2_1580 0.28 +0.4 1,849,013 - Pf6N2E2_1580 0.28 -2.0 1,849,013 - Pf6N2E2_1580 0.28 -0.8 1,849,282 + Pf6N2E2_1580 0.72 -2.2 1,849,424 + -0.1 1,849,426 + -1.0 1,849,426 + -1.2 1,849,426 + +1.9 1,849,541 + +0.8 1,849,541 + -0.6 1,849,682 - +0.4 1,849,691 - +0.9
Or see this region's nucleotide sequence