Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1282

Experiment: LB no stress 48 hours

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1280 and Pf6N2E2_1281 are separated by 22 nucleotidesPf6N2E2_1281 and Pf6N2E2_1282 are separated by 15 nucleotidesPf6N2E2_1282 and Pf6N2E2_1283 are separated by 33 nucleotidesPf6N2E2_1283 and Pf6N2E2_1284 are separated by 35 nucleotides Pf6N2E2_1280 - FIG00958542: hypothetical protein, at 1,510,311 to 1,510,790 _1280 Pf6N2E2_1281 - ATPase involved in DNA repair, at 1,510,813 to 1,511,460 _1281 Pf6N2E2_1282 - Putative outer membrane TonB-dependent receptor associated with haemagglutinin family outer membrane protein, at 1,511,476 to 1,513,173 _1282 Pf6N2E2_1283 - Ferric siderophore transport system, periplasmic binding protein TonB, at 1,513,207 to 1,513,878 _1283 Pf6N2E2_1284 - Biopolymer transport protein ExbD/TolR, at 1,513,914 to 1,514,339 _1284 Position (kb) 1511 1512 1513 1514Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1510.771 kb on + strandat 1510.771 kb on + strandat 1510.772 kb on - strandat 1510.772 kb on - strandat 1510.856 kb on + strandat 1510.856 kb on + strandat 1510.857 kb on - strandat 1510.961 kb on + strand, within Pf6N2E2_1281at 1510.962 kb on - strand, within Pf6N2E2_1281at 1511.109 kb on - strand, within Pf6N2E2_1281at 1511.109 kb on - strand, within Pf6N2E2_1281at 1511.441 kb on + strandat 1511.588 kb on + strandat 1511.588 kb on + strandat 1511.588 kb on + strandat 1511.589 kb on - strandat 1511.589 kb on - strandat 1511.606 kb on + strandat 1511.607 kb on - strandat 1511.681 kb on + strand, within Pf6N2E2_1282at 1511.681 kb on + strand, within Pf6N2E2_1282at 1511.681 kb on + strand, within Pf6N2E2_1282at 1511.681 kb on + strand, within Pf6N2E2_1282at 1511.681 kb on + strand, within Pf6N2E2_1282at 1511.682 kb on - strand, within Pf6N2E2_1282at 1511.682 kb on - strand, within Pf6N2E2_1282at 1511.687 kb on + strand, within Pf6N2E2_1282at 1511.867 kb on + strand, within Pf6N2E2_1282at 1511.868 kb on - strand, within Pf6N2E2_1282at 1511.963 kb on + strand, within Pf6N2E2_1282at 1511.963 kb on + strand, within Pf6N2E2_1282at 1512.143 kb on + strand, within Pf6N2E2_1282at 1512.144 kb on - strand, within Pf6N2E2_1282at 1512.168 kb on + strand, within Pf6N2E2_1282at 1512.272 kb on + strand, within Pf6N2E2_1282at 1512.272 kb on + strand, within Pf6N2E2_1282at 1512.272 kb on + strand, within Pf6N2E2_1282at 1512.374 kb on + strand, within Pf6N2E2_1282at 1512.375 kb on - strand, within Pf6N2E2_1282at 1512.375 kb on - strand, within Pf6N2E2_1282at 1512.743 kb on + strand, within Pf6N2E2_1282at 1512.743 kb on + strand, within Pf6N2E2_1282at 1512.813 kb on + strand, within Pf6N2E2_1282at 1512.905 kb on + strand, within Pf6N2E2_1282at 1512.905 kb on + strand, within Pf6N2E2_1282at 1512.906 kb on - strand, within Pf6N2E2_1282at 1512.906 kb on - strand, within Pf6N2E2_1282at 1513.031 kb on - strandat 1513.031 kb on - strandat 1513.200 kb on + strandat 1513.200 kb on + strandat 1513.246 kb on + strandat 1513.247 kb on - strandat 1513.247 kb on - strandat 1513.472 kb on + strand, within Pf6N2E2_1283at 1513.472 kb on + strand, within Pf6N2E2_1283at 1513.473 kb on - strand, within Pf6N2E2_1283at 1513.473 kb on - strand, within Pf6N2E2_1283at 1514.002 kb on + strand, within Pf6N2E2_1284

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Per-strain Table

Position Strand Gene LocusTag Fraction LB no stress 48 hours
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1,510,771 + +0.4
1,510,771 + +0.5
1,510,772 - +0.8
1,510,772 - -0.2
1,510,856 + +0.6
1,510,856 + -0.3
1,510,857 - +0.2
1,510,961 + Pf6N2E2_1281 0.23 +0.7
1,510,962 - Pf6N2E2_1281 0.23 -0.3
1,511,109 - Pf6N2E2_1281 0.46 +1.1
1,511,109 - Pf6N2E2_1281 0.46 +1.2
1,511,441 + +0.0
1,511,588 + -0.9
1,511,588 + -0.2
1,511,588 + +0.2
1,511,589 - +0.4
1,511,589 - -1.1
1,511,606 + +0.9
1,511,607 - -1.1
1,511,681 + Pf6N2E2_1282 0.12 -3.0
1,511,681 + Pf6N2E2_1282 0.12 -0.1
1,511,681 + Pf6N2E2_1282 0.12 -0.6
1,511,681 + Pf6N2E2_1282 0.12 -0.6
1,511,681 + Pf6N2E2_1282 0.12 -0.7
1,511,682 - Pf6N2E2_1282 0.12 -0.8
1,511,682 - Pf6N2E2_1282 0.12 +0.6
1,511,687 + Pf6N2E2_1282 0.12 -0.4
1,511,867 + Pf6N2E2_1282 0.23 -0.3
1,511,868 - Pf6N2E2_1282 0.23 -1.8
1,511,963 + Pf6N2E2_1282 0.29 +2.2
1,511,963 + Pf6N2E2_1282 0.29 -0.3
1,512,143 + Pf6N2E2_1282 0.39 -0.4
1,512,144 - Pf6N2E2_1282 0.39 +0.2
1,512,168 + Pf6N2E2_1282 0.41 -1.3
1,512,272 + Pf6N2E2_1282 0.47 -0.9
1,512,272 + Pf6N2E2_1282 0.47 -0.7
1,512,272 + Pf6N2E2_1282 0.47 +0.4
1,512,374 + Pf6N2E2_1282 0.53 -0.1
1,512,375 - Pf6N2E2_1282 0.53 -0.4
1,512,375 - Pf6N2E2_1282 0.53 -1.4
1,512,743 + Pf6N2E2_1282 0.75 -1.3
1,512,743 + Pf6N2E2_1282 0.75 -0.1
1,512,813 + Pf6N2E2_1282 0.79 -1.6
1,512,905 + Pf6N2E2_1282 0.84 +2.2
1,512,905 + Pf6N2E2_1282 0.84 -0.7
1,512,906 - Pf6N2E2_1282 0.84 +0.2
1,512,906 - Pf6N2E2_1282 0.84 -2.2
1,513,031 - +0.6
1,513,031 - +1.3
1,513,200 + -1.4
1,513,200 + -0.9
1,513,246 + -1.6
1,513,247 - -0.4
1,513,247 - -0.4
1,513,472 + Pf6N2E2_1283 0.39 +1.2
1,513,472 + Pf6N2E2_1283 0.39 +0.6
1,513,473 - Pf6N2E2_1283 0.40 -1.4
1,513,473 - Pf6N2E2_1283 0.40 -0.5
1,514,002 + Pf6N2E2_1284 0.21 -2.1

Or see this region's nucleotide sequence