Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_118 and Pf6N2E2_119 overlap by 4 nucleotides Pf6N2E2_119 and Pf6N2E2_120 overlap by 4 nucleotides
Pf6N2E2_118 - FIG00961753: hypothetical protein, at 164,733 to 166,103
_118
Pf6N2E2_119 - Glycosyltransferase, at 166,100 to 167,203
_119
Pf6N2E2_120 - Tyrosine-protein kinase Wzc (EC 2.7.10.2), at 167,200 to 168,792
_120
Position (kb) 166
167
168 Strain fitness (log2 ratio) -2
-1
0
1
2 at 165.172 kb on + strand, within Pf6N2E2_118 at 165.172 kb on + strand, within Pf6N2E2_118 at 165.172 kb on + strand, within Pf6N2E2_118 at 165.172 kb on + strand, within Pf6N2E2_118 at 165.173 kb on - strand, within Pf6N2E2_118 at 165.364 kb on + strand, within Pf6N2E2_118 at 165.384 kb on - strand, within Pf6N2E2_118 at 165.474 kb on + strand, within Pf6N2E2_118 at 165.475 kb on - strand, within Pf6N2E2_118 at 165.589 kb on + strand, within Pf6N2E2_118 at 165.778 kb on + strand, within Pf6N2E2_118 at 165.812 kb on + strand, within Pf6N2E2_118 at 165.813 kb on - strand, within Pf6N2E2_118 at 165.982 kb on + strand at 166.131 kb on + strand at 166.131 kb on + strand at 166.132 kb on - strand at 166.294 kb on + strand, within Pf6N2E2_119 at 166.310 kb on + strand, within Pf6N2E2_119 at 166.310 kb on + strand, within Pf6N2E2_119 at 166.311 kb on - strand, within Pf6N2E2_119 at 166.311 kb on - strand, within Pf6N2E2_119 at 166.311 kb on - strand, within Pf6N2E2_119 at 166.455 kb on + strand, within Pf6N2E2_119 at 166.822 kb on + strand, within Pf6N2E2_119 at 166.823 kb on - strand, within Pf6N2E2_119 at 166.824 kb on + strand, within Pf6N2E2_119 at 166.824 kb on + strand, within Pf6N2E2_119 at 166.824 kb on + strand, within Pf6N2E2_119 at 166.824 kb on + strand, within Pf6N2E2_119 at 166.825 kb on - strand, within Pf6N2E2_119 at 166.825 kb on - strand, within Pf6N2E2_119 at 166.825 kb on - strand, within Pf6N2E2_119 at 166.825 kb on - strand, within Pf6N2E2_119 at 166.825 kb on - strand, within Pf6N2E2_119 at 166.825 kb on - strand, within Pf6N2E2_119 at 167.127 kb on + strand at 167.127 kb on + strand at 167.360 kb on + strand, within Pf6N2E2_120 at 167.360 kb on + strand, within Pf6N2E2_120 at 167.360 kb on + strand, within Pf6N2E2_120 at 167.360 kb on + strand, within Pf6N2E2_120 at 167.361 kb on - strand, within Pf6N2E2_120 at 167.361 kb on - strand, within Pf6N2E2_120 at 167.397 kb on + strand, within Pf6N2E2_120 at 167.531 kb on + strand, within Pf6N2E2_120 at 167.532 kb on - strand, within Pf6N2E2_120 at 167.532 kb on - strand, within Pf6N2E2_120 at 167.804 kb on + strand, within Pf6N2E2_120 at 167.906 kb on + strand, within Pf6N2E2_120 at 167.906 kb on + strand, within Pf6N2E2_120 at 167.906 kb on + strand, within Pf6N2E2_120 at 167.907 kb on - strand, within Pf6N2E2_120 at 167.907 kb on - strand, within Pf6N2E2_120 at 167.954 kb on + strand, within Pf6N2E2_120 at 167.955 kb on - strand, within Pf6N2E2_120 at 167.994 kb on - strand, within Pf6N2E2_120 at 168.125 kb on + strand, within Pf6N2E2_120 at 168.126 kb on - strand, within Pf6N2E2_120 at 168.146 kb on + strand, within Pf6N2E2_120 at 168.146 kb on + strand, within Pf6N2E2_120 at 168.146 kb on + strand, within Pf6N2E2_120 at 168.146 kb on + strand, within Pf6N2E2_120 at 168.147 kb on - strand, within Pf6N2E2_120 at 168.197 kb on + strand, within Pf6N2E2_120 at 168.197 kb on + strand, within Pf6N2E2_120
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 165,172 + Pf6N2E2_118 0.32 +0.4 165,172 + Pf6N2E2_118 0.32 +0.7 165,172 + Pf6N2E2_118 0.32 +0.6 165,172 + Pf6N2E2_118 0.32 +1.1 165,173 - Pf6N2E2_118 0.32 +1.1 165,364 + Pf6N2E2_118 0.46 -0.4 165,384 - Pf6N2E2_118 0.47 -0.7 165,474 + Pf6N2E2_118 0.54 +0.1 165,475 - Pf6N2E2_118 0.54 +1.2 165,589 + Pf6N2E2_118 0.62 +0.3 165,778 + Pf6N2E2_118 0.76 +0.0 165,812 + Pf6N2E2_118 0.79 +0.0 165,813 - Pf6N2E2_118 0.79 +0.2 165,982 + -1.3 166,131 + -0.8 166,131 + -0.9 166,132 - +0.8 166,294 + Pf6N2E2_119 0.18 +0.0 166,310 + Pf6N2E2_119 0.19 -0.1 166,310 + Pf6N2E2_119 0.19 +1.5 166,311 - Pf6N2E2_119 0.19 +0.9 166,311 - Pf6N2E2_119 0.19 +0.7 166,311 - Pf6N2E2_119 0.19 +1.0 166,455 + Pf6N2E2_119 0.32 +1.6 166,822 + Pf6N2E2_119 0.65 -1.2 166,823 - Pf6N2E2_119 0.65 -0.1 166,824 + Pf6N2E2_119 0.66 +1.6 166,824 + Pf6N2E2_119 0.66 -1.6 166,824 + Pf6N2E2_119 0.66 -0.3 166,824 + Pf6N2E2_119 0.66 +1.0 166,825 - Pf6N2E2_119 0.66 +0.1 166,825 - Pf6N2E2_119 0.66 +0.8 166,825 - Pf6N2E2_119 0.66 +1.4 166,825 - Pf6N2E2_119 0.66 +1.2 166,825 - Pf6N2E2_119 0.66 +0.6 166,825 - Pf6N2E2_119 0.66 -0.1 167,127 + -1.2 167,127 + +1.6 167,360 + Pf6N2E2_120 0.10 -0.4 167,360 + Pf6N2E2_120 0.10 +0.2 167,360 + Pf6N2E2_120 0.10 +1.8 167,360 + Pf6N2E2_120 0.10 +0.8 167,361 - Pf6N2E2_120 0.10 +0.1 167,361 - Pf6N2E2_120 0.10 +0.3 167,397 + Pf6N2E2_120 0.12 -0.7 167,531 + Pf6N2E2_120 0.21 -1.4 167,532 - Pf6N2E2_120 0.21 +0.3 167,532 - Pf6N2E2_120 0.21 -2.2 167,804 + Pf6N2E2_120 0.38 -0.2 167,906 + Pf6N2E2_120 0.44 -1.0 167,906 + Pf6N2E2_120 0.44 +1.5 167,906 + Pf6N2E2_120 0.44 -0.1 167,907 - Pf6N2E2_120 0.44 +0.1 167,907 - Pf6N2E2_120 0.44 -1.8 167,954 + Pf6N2E2_120 0.47 -0.9 167,955 - Pf6N2E2_120 0.47 +0.3 167,994 - Pf6N2E2_120 0.50 -0.8 168,125 + Pf6N2E2_120 0.58 +0.5 168,126 - Pf6N2E2_120 0.58 -0.1 168,146 + Pf6N2E2_120 0.59 -0.9 168,146 + Pf6N2E2_120 0.59 -0.5 168,146 + Pf6N2E2_120 0.59 -0.4 168,146 + Pf6N2E2_120 0.59 -0.4 168,147 - Pf6N2E2_120 0.59 -1.2 168,197 + Pf6N2E2_120 0.63 -1.6 168,197 + Pf6N2E2_120 0.63 +0.8
Or see this region's nucleotide sequence