Experiment: R2A with Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35275 and RR42_RS35280 overlap by 4 nucleotides RR42_RS35280 and RR42_RS35285 are separated by 17 nucleotides
RR42_RS35275: RR42_RS35275 - membrane protein, at 3,368,843 to 3,369,814
_RS35275
RR42_RS35280: RR42_RS35280 - hypothetical protein, at 3,369,811 to 3,370,662
_RS35280
RR42_RS35285: RR42_RS35285 - hopanoid biosynthesis associated radical SAM protein HpnJ, at 3,370,680 to 3,372,101
_RS35285
Position (kb)
3369
3370
3371 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3368.844 kb on + strand at 3368.844 kb on + strand at 3368.845 kb on - strand at 3368.845 kb on - strand at 3368.845 kb on - strand at 3369.292 kb on + strand, within RR42_RS35275 at 3369.292 kb on + strand, within RR42_RS35275 at 3369.293 kb on - strand, within RR42_RS35275 at 3369.426 kb on + strand, within RR42_RS35275 at 3369.773 kb on + strand at 3369.798 kb on + strand at 3369.799 kb on - strand at 3369.951 kb on + strand, within RR42_RS35280 at 3369.951 kb on + strand, within RR42_RS35280 at 3369.951 kb on + strand, within RR42_RS35280 at 3369.951 kb on + strand, within RR42_RS35280 at 3369.951 kb on + strand, within RR42_RS35280 at 3369.951 kb on + strand, within RR42_RS35280 at 3369.951 kb on + strand, within RR42_RS35280 at 3369.952 kb on - strand, within RR42_RS35280 at 3369.952 kb on - strand, within RR42_RS35280 at 3369.952 kb on - strand, within RR42_RS35280 at 3369.952 kb on - strand, within RR42_RS35280 at 3369.952 kb on - strand, within RR42_RS35280 at 3369.952 kb on - strand, within RR42_RS35280 at 3369.952 kb on - strand, within RR42_RS35280 at 3369.952 kb on - strand, within RR42_RS35280 at 3369.992 kb on + strand, within RR42_RS35280 at 3369.992 kb on + strand, within RR42_RS35280 at 3369.992 kb on + strand, within RR42_RS35280 at 3369.993 kb on - strand, within RR42_RS35280 at 3369.993 kb on - strand, within RR42_RS35280 at 3370.083 kb on - strand, within RR42_RS35280 at 3370.801 kb on + strand at 3370.801 kb on + strand at 3370.802 kb on - strand at 3370.811 kb on + strand at 3370.811 kb on + strand at 3370.812 kb on - strand at 3370.812 kb on - strand at 3370.812 kb on - strand at 3370.812 kb on - strand at 3370.812 kb on - strand at 3370.813 kb on + strand at 3370.813 kb on + strand at 3370.813 kb on + strand at 3370.813 kb on + strand at 3370.813 kb on + strand at 3370.813 kb on + strand at 3370.813 kb on + strand at 3370.814 kb on - strand at 3370.834 kb on + strand, within RR42_RS35285 at 3370.834 kb on + strand, within RR42_RS35285 at 3370.834 kb on + strand, within RR42_RS35285 at 3370.834 kb on + strand, within RR42_RS35285 at 3370.834 kb on + strand, within RR42_RS35285 at 3370.834 kb on + strand, within RR42_RS35285 at 3370.834 kb on + strand, within RR42_RS35285 at 3370.834 kb on + strand, within RR42_RS35285 at 3370.835 kb on - strand, within RR42_RS35285 at 3370.835 kb on - strand, within RR42_RS35285 at 3370.835 kb on - strand, within RR42_RS35285 at 3370.835 kb on - strand, within RR42_RS35285 at 3370.835 kb on - strand, within RR42_RS35285 at 3370.952 kb on - strand, within RR42_RS35285 at 3370.954 kb on - strand, within RR42_RS35285 at 3370.954 kb on - strand, within RR42_RS35285 at 3370.954 kb on - strand, within RR42_RS35285 at 3370.954 kb on - strand, within RR42_RS35285 at 3371.243 kb on - strand, within RR42_RS35285 at 3371.243 kb on - strand, within RR42_RS35285 at 3371.499 kb on - strand, within RR42_RS35285 at 3371.500 kb on + strand, within RR42_RS35285 at 3371.501 kb on - strand, within RR42_RS35285 at 3371.501 kb on - strand, within RR42_RS35285 at 3371.501 kb on - strand, within RR42_RS35285 at 3371.512 kb on + strand, within RR42_RS35285 at 3371.539 kb on + strand, within RR42_RS35285 at 3371.539 kb on + strand, within RR42_RS35285 at 3371.540 kb on - strand, within RR42_RS35285 at 3371.566 kb on + strand, within RR42_RS35285 at 3371.566 kb on + strand, within RR42_RS35285 at 3371.567 kb on - strand, within RR42_RS35285 at 3371.567 kb on - strand, within RR42_RS35285 at 3371.653 kb on + strand, within RR42_RS35285 at 3371.654 kb on - strand, within RR42_RS35285
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.5 mM remove 3,368,844 + +0.8 3,368,844 + +0.5 3,368,845 - -0.8 3,368,845 - +1.0 3,368,845 - +0.5 3,369,292 + RR42_RS35275 0.46 +0.4 3,369,292 + RR42_RS35275 0.46 +0.0 3,369,293 - RR42_RS35275 0.46 -0.7 3,369,426 + RR42_RS35275 0.60 +0.6 3,369,773 + -3.1 3,369,798 + -1.1 3,369,799 - -0.2 3,369,951 + RR42_RS35280 0.16 +0.1 3,369,951 + RR42_RS35280 0.16 -0.8 3,369,951 + RR42_RS35280 0.16 -0.0 3,369,951 + RR42_RS35280 0.16 +0.4 3,369,951 + RR42_RS35280 0.16 +0.3 3,369,951 + RR42_RS35280 0.16 -0.2 3,369,951 + RR42_RS35280 0.16 -0.2 3,369,952 - RR42_RS35280 0.17 -0.7 3,369,952 - RR42_RS35280 0.17 -1.3 3,369,952 - RR42_RS35280 0.17 -0.5 3,369,952 - RR42_RS35280 0.17 -0.6 3,369,952 - RR42_RS35280 0.17 +0.6 3,369,952 - RR42_RS35280 0.17 +0.2 3,369,952 - RR42_RS35280 0.17 -1.1 3,369,952 - RR42_RS35280 0.17 +0.2 3,369,992 + RR42_RS35280 0.21 +0.0 3,369,992 + RR42_RS35280 0.21 -0.8 3,369,992 + RR42_RS35280 0.21 -2.0 3,369,993 - RR42_RS35280 0.21 -1.1 3,369,993 - RR42_RS35280 0.21 -0.9 3,370,083 - RR42_RS35280 0.32 -0.7 3,370,801 + +0.9 3,370,801 + -0.8 3,370,802 - -0.2 3,370,811 + -2.1 3,370,811 + +0.0 3,370,812 - +0.5 3,370,812 - +0.3 3,370,812 - -0.0 3,370,812 - +0.2 3,370,812 - -0.5 3,370,813 + -0.3 3,370,813 + -1.7 3,370,813 + -1.0 3,370,813 + -0.1 3,370,813 + +0.1 3,370,813 + -0.1 3,370,813 + +1.0 3,370,814 - -0.2 3,370,834 + RR42_RS35285 0.11 -1.1 3,370,834 + RR42_RS35285 0.11 +1.0 3,370,834 + RR42_RS35285 0.11 -0.7 3,370,834 + RR42_RS35285 0.11 +0.5 3,370,834 + RR42_RS35285 0.11 +0.1 3,370,834 + RR42_RS35285 0.11 -0.7 3,370,834 + RR42_RS35285 0.11 +0.2 3,370,834 + RR42_RS35285 0.11 +0.1 3,370,835 - RR42_RS35285 0.11 +0.7 3,370,835 - RR42_RS35285 0.11 +0.2 3,370,835 - RR42_RS35285 0.11 -0.7 3,370,835 - RR42_RS35285 0.11 +0.2 3,370,835 - RR42_RS35285 0.11 +0.5 3,370,952 - RR42_RS35285 0.19 +0.0 3,370,954 - RR42_RS35285 0.19 -0.3 3,370,954 - RR42_RS35285 0.19 +0.9 3,370,954 - RR42_RS35285 0.19 +0.1 3,370,954 - RR42_RS35285 0.19 -0.6 3,371,243 - RR42_RS35285 0.40 +0.3 3,371,243 - RR42_RS35285 0.40 +1.0 3,371,499 - RR42_RS35285 0.58 +0.2 3,371,500 + RR42_RS35285 0.58 +0.0 3,371,501 - RR42_RS35285 0.58 +0.1 3,371,501 - RR42_RS35285 0.58 -0.9 3,371,501 - RR42_RS35285 0.58 +0.6 3,371,512 + RR42_RS35285 0.59 -0.6 3,371,539 + RR42_RS35285 0.60 -0.4 3,371,539 + RR42_RS35285 0.60 -1.8 3,371,540 - RR42_RS35285 0.60 +0.5 3,371,566 + RR42_RS35285 0.62 -0.2 3,371,566 + RR42_RS35285 0.62 +0.6 3,371,567 - RR42_RS35285 0.62 -0.5 3,371,567 - RR42_RS35285 0.62 -0.2 3,371,653 + RR42_RS35285 0.68 -0.3 3,371,654 - RR42_RS35285 0.68 -0.7
Or see this region's nucleotide sequence