Experiment: R2A with Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS34990 and RR42_RS34995 are separated by 55 nucleotides RR42_RS34995 and RR42_RS35000 are separated by 152 nucleotides RR42_RS35000 and RR42_RS35005 are separated by 137 nucleotides
RR42_RS34990: RR42_RS34990 - ABC transporter permease, at 3,301,569 to 3,302,972
_RS34990
RR42_RS34995: RR42_RS34995 - ABC transporter permease, at 3,303,028 to 3,303,582
_RS34995
RR42_RS35000: RR42_RS35000 - ABC transporter substrate-binding protein, at 3,303,735 to 3,304,682
_RS35000
RR42_RS35005: RR42_RS35005 - L-idonate 5-dehydrogenase, at 3,304,820 to 3,305,848
_RS35005
Position (kb)
3303
3304
3305 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3302.742 kb on - strand, within RR42_RS34990 at 3302.744 kb on - strand, within RR42_RS34990 at 3302.886 kb on - strand at 3302.886 kb on - strand at 3302.886 kb on - strand at 3302.886 kb on - strand at 3302.887 kb on + strand at 3302.887 kb on + strand at 3302.887 kb on + strand at 3302.887 kb on + strand at 3302.888 kb on - strand at 3302.888 kb on - strand at 3302.888 kb on - strand at 3303.149 kb on + strand, within RR42_RS34995 at 3303.149 kb on + strand, within RR42_RS34995 at 3303.150 kb on - strand, within RR42_RS34995 at 3303.150 kb on - strand, within RR42_RS34995 at 3303.152 kb on + strand, within RR42_RS34995 at 3303.153 kb on - strand, within RR42_RS34995 at 3303.153 kb on - strand, within RR42_RS34995 at 3303.153 kb on - strand, within RR42_RS34995 at 3303.153 kb on - strand, within RR42_RS34995 at 3303.153 kb on - strand, within RR42_RS34995 at 3303.212 kb on + strand, within RR42_RS34995 at 3303.212 kb on + strand, within RR42_RS34995 at 3303.213 kb on - strand, within RR42_RS34995 at 3303.213 kb on - strand, within RR42_RS34995 at 3303.213 kb on - strand, within RR42_RS34995 at 3303.237 kb on - strand, within RR42_RS34995 at 3303.311 kb on + strand, within RR42_RS34995 at 3303.312 kb on - strand, within RR42_RS34995 at 3303.312 kb on - strand, within RR42_RS34995 at 3303.390 kb on - strand, within RR42_RS34995 at 3303.556 kb on - strand at 3303.556 kb on - strand at 3303.556 kb on - strand at 3303.696 kb on + strand at 3303.696 kb on + strand at 3303.821 kb on - strand at 3304.051 kb on + strand, within RR42_RS35000 at 3304.123 kb on + strand, within RR42_RS35000 at 3304.123 kb on + strand, within RR42_RS35000 at 3304.124 kb on - strand, within RR42_RS35000 at 3304.151 kb on + strand, within RR42_RS35000 at 3304.152 kb on - strand, within RR42_RS35000 at 3304.152 kb on - strand, within RR42_RS35000 at 3304.184 kb on - strand, within RR42_RS35000 at 3304.273 kb on + strand, within RR42_RS35000 at 3304.276 kb on + strand, within RR42_RS35000 at 3304.277 kb on - strand, within RR42_RS35000 at 3304.293 kb on - strand, within RR42_RS35000 at 3304.336 kb on + strand, within RR42_RS35000 at 3304.337 kb on - strand, within RR42_RS35000 at 3304.340 kb on + strand, within RR42_RS35000 at 3304.343 kb on - strand, within RR42_RS35000 at 3304.353 kb on - strand, within RR42_RS35000 at 3304.394 kb on - strand, within RR42_RS35000 at 3304.394 kb on - strand, within RR42_RS35000 at 3304.396 kb on + strand, within RR42_RS35000 at 3304.396 kb on + strand, within RR42_RS35000 at 3304.396 kb on + strand, within RR42_RS35000 at 3304.397 kb on - strand, within RR42_RS35000 at 3304.397 kb on - strand, within RR42_RS35000 at 3304.397 kb on - strand, within RR42_RS35000 at 3304.454 kb on + strand, within RR42_RS35000 at 3304.609 kb on + strand at 3304.609 kb on + strand at 3304.610 kb on - strand at 3304.822 kb on - strand at 3304.822 kb on - strand at 3304.822 kb on - strand at 3304.838 kb on + strand at 3304.839 kb on - strand at 3304.998 kb on + strand, within RR42_RS35005 at 3304.999 kb on - strand, within RR42_RS35005 at 3304.999 kb on - strand, within RR42_RS35005 at 3304.999 kb on - strand, within RR42_RS35005 at 3305.419 kb on + strand, within RR42_RS35005 at 3305.420 kb on - strand, within RR42_RS35005 at 3305.493 kb on + strand, within RR42_RS35005 at 3305.494 kb on - strand, within RR42_RS35005 at 3305.494 kb on - strand, within RR42_RS35005
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.5 mM remove 3,302,742 - RR42_RS34990 0.84 +0.4 3,302,744 - RR42_RS34990 0.84 -1.6 3,302,886 - -0.1 3,302,886 - -0.2 3,302,886 - -2.0 3,302,886 - -0.8 3,302,887 + -0.2 3,302,887 + -0.0 3,302,887 + -0.0 3,302,887 + -1.3 3,302,888 - -0.1 3,302,888 - +0.5 3,302,888 - +0.5 3,303,149 + RR42_RS34995 0.22 -1.3 3,303,149 + RR42_RS34995 0.22 +1.9 3,303,150 - RR42_RS34995 0.22 +1.0 3,303,150 - RR42_RS34995 0.22 -0.5 3,303,152 + RR42_RS34995 0.22 -0.9 3,303,153 - RR42_RS34995 0.23 -0.2 3,303,153 - RR42_RS34995 0.23 -0.2 3,303,153 - RR42_RS34995 0.23 +0.1 3,303,153 - RR42_RS34995 0.23 -2.9 3,303,153 - RR42_RS34995 0.23 -0.4 3,303,212 + RR42_RS34995 0.33 +0.0 3,303,212 + RR42_RS34995 0.33 -1.2 3,303,213 - RR42_RS34995 0.33 +1.1 3,303,213 - RR42_RS34995 0.33 -0.2 3,303,213 - RR42_RS34995 0.33 -0.8 3,303,237 - RR42_RS34995 0.38 +0.1 3,303,311 + RR42_RS34995 0.51 -0.4 3,303,312 - RR42_RS34995 0.51 -2.7 3,303,312 - RR42_RS34995 0.51 -0.6 3,303,390 - RR42_RS34995 0.65 +0.5 3,303,556 - -0.7 3,303,556 - +0.0 3,303,556 - +0.9 3,303,696 + -0.0 3,303,696 + -1.8 3,303,821 - -0.6 3,304,051 + RR42_RS35000 0.33 +1.2 3,304,123 + RR42_RS35000 0.41 -0.3 3,304,123 + RR42_RS35000 0.41 +0.2 3,304,124 - RR42_RS35000 0.41 -1.8 3,304,151 + RR42_RS35000 0.44 -1.1 3,304,152 - RR42_RS35000 0.44 -0.3 3,304,152 - RR42_RS35000 0.44 -0.7 3,304,184 - RR42_RS35000 0.47 -0.5 3,304,273 + RR42_RS35000 0.57 -0.7 3,304,276 + RR42_RS35000 0.57 +0.6 3,304,277 - RR42_RS35000 0.57 +0.0 3,304,293 - RR42_RS35000 0.59 -0.4 3,304,336 + RR42_RS35000 0.63 +1.3 3,304,337 - RR42_RS35000 0.64 +0.5 3,304,340 + RR42_RS35000 0.64 -2.8 3,304,343 - RR42_RS35000 0.64 +0.1 3,304,353 - RR42_RS35000 0.65 -0.0 3,304,394 - RR42_RS35000 0.70 +1.1 3,304,394 - RR42_RS35000 0.70 -0.4 3,304,396 + RR42_RS35000 0.70 +0.7 3,304,396 + RR42_RS35000 0.70 +0.4 3,304,396 + RR42_RS35000 0.70 +0.4 3,304,397 - RR42_RS35000 0.70 -0.2 3,304,397 - RR42_RS35000 0.70 -0.6 3,304,397 - RR42_RS35000 0.70 -1.1 3,304,454 + RR42_RS35000 0.76 -0.9 3,304,609 + +0.3 3,304,609 + -0.7 3,304,610 - -1.9 3,304,822 - -0.1 3,304,822 - +1.2 3,304,822 - -0.3 3,304,838 + +0.9 3,304,839 - -0.4 3,304,998 + RR42_RS35005 0.17 +1.8 3,304,999 - RR42_RS35005 0.17 -0.9 3,304,999 - RR42_RS35005 0.17 -0.2 3,304,999 - RR42_RS35005 0.17 +0.1 3,305,419 + RR42_RS35005 0.58 +1.8 3,305,420 - RR42_RS35005 0.58 -0.9 3,305,493 + RR42_RS35005 0.65 -0.1 3,305,494 - RR42_RS35005 0.66 +0.1 3,305,494 - RR42_RS35005 0.66 -0.5
Or see this region's nucleotide sequence