Experiment: R2A with Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32565 and RR42_RS32570 are separated by 123 nucleotides RR42_RS32570 and RR42_RS32575 overlap by 1 nucleotides
RR42_RS32565: RR42_RS32565 - MFS transporter, at 2,702,087 to 2,703,424
_RS32565
RR42_RS32570: RR42_RS32570 - Vanillate O-demethylase oxidoreductase, at 2,703,548 to 2,704,492
_RS32570
RR42_RS32575: RR42_RS32575 - (2Fe-2S)-binding protein, at 2,704,492 to 2,705,538
_RS32575
Position (kb)
2703
2704
2705 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2702.616 kb on + strand, within RR42_RS32565 at 2702.616 kb on + strand, within RR42_RS32565 at 2702.617 kb on - strand, within RR42_RS32565 at 2702.663 kb on - strand, within RR42_RS32565 at 2702.747 kb on - strand, within RR42_RS32565 at 2702.913 kb on + strand, within RR42_RS32565 at 2702.914 kb on - strand, within RR42_RS32565 at 2703.018 kb on + strand, within RR42_RS32565 at 2703.289 kb on + strand, within RR42_RS32565 at 2703.290 kb on - strand, within RR42_RS32565 at 2703.290 kb on - strand, within RR42_RS32565 at 2703.290 kb on - strand, within RR42_RS32565 at 2703.430 kb on - strand at 2703.430 kb on - strand at 2703.430 kb on - strand at 2703.443 kb on + strand at 2703.444 kb on - strand at 2703.454 kb on - strand at 2703.519 kb on + strand at 2703.550 kb on - strand at 2703.637 kb on - strand at 2703.849 kb on + strand, within RR42_RS32570 at 2703.936 kb on + strand, within RR42_RS32570 at 2703.936 kb on + strand, within RR42_RS32570 at 2703.937 kb on - strand, within RR42_RS32570 at 2704.011 kb on + strand, within RR42_RS32570 at 2704.012 kb on - strand, within RR42_RS32570 at 2704.012 kb on - strand, within RR42_RS32570 at 2704.089 kb on + strand, within RR42_RS32570 at 2704.090 kb on - strand, within RR42_RS32570 at 2704.384 kb on + strand, within RR42_RS32570 at 2704.737 kb on - strand, within RR42_RS32575 at 2704.814 kb on + strand, within RR42_RS32575 at 2704.971 kb on + strand, within RR42_RS32575 at 2704.972 kb on - strand, within RR42_RS32575 at 2704.972 kb on - strand, within RR42_RS32575 at 2705.062 kb on + strand, within RR42_RS32575 at 2705.063 kb on + strand, within RR42_RS32575 at 2705.063 kb on + strand, within RR42_RS32575 at 2705.063 kb on + strand, within RR42_RS32575 at 2705.063 kb on + strand, within RR42_RS32575 at 2705.063 kb on + strand, within RR42_RS32575 at 2705.063 kb on + strand, within RR42_RS32575 at 2705.064 kb on - strand, within RR42_RS32575 at 2705.064 kb on - strand, within RR42_RS32575 at 2705.064 kb on - strand, within RR42_RS32575 at 2705.064 kb on - strand, within RR42_RS32575 at 2705.123 kb on + strand, within RR42_RS32575 at 2705.123 kb on + strand, within RR42_RS32575 at 2705.123 kb on + strand, within RR42_RS32575 at 2705.123 kb on + strand, within RR42_RS32575 at 2705.124 kb on - strand, within RR42_RS32575 at 2705.124 kb on - strand, within RR42_RS32575 at 2705.124 kb on - strand, within RR42_RS32575 at 2705.126 kb on + strand, within RR42_RS32575 at 2705.235 kb on - strand, within RR42_RS32575
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.5 mM remove 2,702,616 + RR42_RS32565 0.40 +0.2 2,702,616 + RR42_RS32565 0.40 -0.1 2,702,617 - RR42_RS32565 0.40 -0.3 2,702,663 - RR42_RS32565 0.43 -0.2 2,702,747 - RR42_RS32565 0.49 +0.5 2,702,913 + RR42_RS32565 0.62 +0.2 2,702,914 - RR42_RS32565 0.62 +0.1 2,703,018 + RR42_RS32565 0.70 -1.2 2,703,289 + RR42_RS32565 0.90 -2.6 2,703,290 - RR42_RS32565 0.90 -2.2 2,703,290 - RR42_RS32565 0.90 +2.3 2,703,290 - RR42_RS32565 0.90 +0.3 2,703,430 - -0.2 2,703,430 - +1.3 2,703,430 - +0.6 2,703,443 + +0.7 2,703,444 - +0.0 2,703,454 - +0.0 2,703,519 + -0.2 2,703,550 - -0.9 2,703,637 - +0.6 2,703,849 + RR42_RS32570 0.32 -0.4 2,703,936 + RR42_RS32570 0.41 -1.2 2,703,936 + RR42_RS32570 0.41 -0.7 2,703,937 - RR42_RS32570 0.41 -2.1 2,704,011 + RR42_RS32570 0.49 +0.3 2,704,012 - RR42_RS32570 0.49 +0.7 2,704,012 - RR42_RS32570 0.49 -1.7 2,704,089 + RR42_RS32570 0.57 -0.1 2,704,090 - RR42_RS32570 0.57 -2.8 2,704,384 + RR42_RS32570 0.88 -1.7 2,704,737 - RR42_RS32575 0.23 +0.0 2,704,814 + RR42_RS32575 0.31 -0.7 2,704,971 + RR42_RS32575 0.46 -1.2 2,704,972 - RR42_RS32575 0.46 -1.8 2,704,972 - RR42_RS32575 0.46 -0.6 2,705,062 + RR42_RS32575 0.54 -1.5 2,705,063 + RR42_RS32575 0.55 +0.2 2,705,063 + RR42_RS32575 0.55 -0.7 2,705,063 + RR42_RS32575 0.55 +0.1 2,705,063 + RR42_RS32575 0.55 -0.0 2,705,063 + RR42_RS32575 0.55 +0.7 2,705,063 + RR42_RS32575 0.55 +0.5 2,705,064 - RR42_RS32575 0.55 -1.0 2,705,064 - RR42_RS32575 0.55 +0.6 2,705,064 - RR42_RS32575 0.55 -0.2 2,705,064 - RR42_RS32575 0.55 +0.8 2,705,123 + RR42_RS32575 0.60 +0.9 2,705,123 + RR42_RS32575 0.60 +0.7 2,705,123 + RR42_RS32575 0.60 -0.3 2,705,123 + RR42_RS32575 0.60 +0.9 2,705,124 - RR42_RS32575 0.60 +0.4 2,705,124 - RR42_RS32575 0.60 -0.6 2,705,124 - RR42_RS32575 0.60 -1.9 2,705,126 + RR42_RS32575 0.61 -0.4 2,705,235 - RR42_RS32575 0.71 -2.0
Or see this region's nucleotide sequence