Experiment: R2A with Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32250 and RR42_RS32255 are separated by 29 nucleotides RR42_RS32255 and RR42_RS32260 are separated by 53 nucleotides RR42_RS32260 and RR42_RS32265 are separated by 93 nucleotides
RR42_RS32250: RR42_RS32250 - ABC transporter, at 2,623,493 to 2,624,296
_RS32250
RR42_RS32255: RR42_RS32255 - squalene-hopene cyclase, at 2,624,326 to 2,624,964
_RS32255
RR42_RS32260: RR42_RS32260 - squalene--hopene cyclase, at 2,625,018 to 2,627,033
_RS32260
RR42_RS32265: RR42_RS32265 - phytoene synthase, at 2,627,127 to 2,627,975
_RS32265
Position (kb)
2625
2626
2627
2628 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2624.090 kb on - strand, within RR42_RS32250 at 2624.090 kb on - strand, within RR42_RS32250 at 2624.090 kb on - strand, within RR42_RS32250 at 2624.090 kb on - strand, within RR42_RS32250 at 2624.090 kb on - strand, within RR42_RS32250 at 2624.093 kb on + strand, within RR42_RS32250 at 2624.093 kb on + strand, within RR42_RS32250 at 2624.093 kb on + strand, within RR42_RS32250 at 2624.093 kb on + strand, within RR42_RS32250 at 2624.093 kb on + strand, within RR42_RS32250 at 2624.093 kb on + strand, within RR42_RS32250 at 2624.093 kb on + strand, within RR42_RS32250 at 2624.094 kb on - strand, within RR42_RS32250 at 2624.114 kb on + strand, within RR42_RS32250 at 2624.115 kb on - strand, within RR42_RS32250 at 2624.132 kb on + strand, within RR42_RS32250 at 2624.132 kb on + strand, within RR42_RS32250 at 2624.133 kb on - strand, within RR42_RS32250 at 2625.121 kb on + strand at 2625.199 kb on + strand at 2625.200 kb on - strand at 2625.200 kb on - strand at 2625.301 kb on + strand, within RR42_RS32260 at 2625.301 kb on + strand, within RR42_RS32260 at 2625.302 kb on - strand, within RR42_RS32260 at 2625.322 kb on + strand, within RR42_RS32260 at 2625.457 kb on + strand, within RR42_RS32260 at 2625.457 kb on + strand, within RR42_RS32260 at 2625.463 kb on + strand, within RR42_RS32260 at 2625.463 kb on + strand, within RR42_RS32260 at 2625.463 kb on + strand, within RR42_RS32260 at 2625.463 kb on + strand, within RR42_RS32260 at 2625.463 kb on + strand, within RR42_RS32260 at 2625.463 kb on + strand, within RR42_RS32260 at 2625.464 kb on - strand, within RR42_RS32260 at 2625.464 kb on - strand, within RR42_RS32260 at 2625.464 kb on - strand, within RR42_RS32260 at 2625.464 kb on - strand, within RR42_RS32260 at 2625.484 kb on + strand, within RR42_RS32260 at 2625.484 kb on + strand, within RR42_RS32260 at 2625.650 kb on + strand, within RR42_RS32260 at 2625.662 kb on - strand, within RR42_RS32260 at 2625.662 kb on - strand, within RR42_RS32260 at 2625.662 kb on - strand, within RR42_RS32260 at 2625.662 kb on - strand, within RR42_RS32260 at 2625.662 kb on - strand, within RR42_RS32260 at 2625.878 kb on - strand, within RR42_RS32260 at 2626.287 kb on + strand, within RR42_RS32260 at 2626.492 kb on + strand, within RR42_RS32260 at 2626.492 kb on + strand at 2626.492 kb on + strand, within RR42_RS32260 at 2626.493 kb on - strand, within RR42_RS32260 at 2626.493 kb on - strand, within RR42_RS32260 at 2626.493 kb on - strand, within RR42_RS32260 at 2626.493 kb on - strand, within RR42_RS32260 at 2626.608 kb on - strand, within RR42_RS32260 at 2626.608 kb on - strand, within RR42_RS32260 at 2626.711 kb on + strand, within RR42_RS32260 at 2626.711 kb on + strand, within RR42_RS32260 at 2626.712 kb on - strand, within RR42_RS32260 at 2626.806 kb on - strand, within RR42_RS32260 at 2626.843 kb on + strand at 2626.844 kb on - strand at 2626.844 kb on - strand at 2626.861 kb on + strand at 2626.861 kb on + strand at 2627.016 kb on + strand at 2627.066 kb on + strand at 2627.067 kb on - strand at 2627.157 kb on + strand at 2627.240 kb on - strand, within RR42_RS32265 at 2627.314 kb on + strand, within RR42_RS32265 at 2627.315 kb on - strand, within RR42_RS32265 at 2627.317 kb on + strand, within RR42_RS32265 at 2627.317 kb on + strand, within RR42_RS32265 at 2627.318 kb on - strand, within RR42_RS32265 at 2627.434 kb on + strand, within RR42_RS32265 at 2627.435 kb on - strand, within RR42_RS32265 at 2627.435 kb on - strand, within RR42_RS32265 at 2627.521 kb on - strand, within RR42_RS32265 at 2627.593 kb on + strand, within RR42_RS32265 at 2627.593 kb on + strand, within RR42_RS32265 at 2627.594 kb on - strand, within RR42_RS32265 at 2627.594 kb on - strand, within RR42_RS32265 at 2627.594 kb on - strand, within RR42_RS32265 at 2627.601 kb on + strand, within RR42_RS32265 at 2627.601 kb on + strand, within RR42_RS32265 at 2627.601 kb on + strand, within RR42_RS32265 at 2627.602 kb on - strand, within RR42_RS32265 at 2627.607 kb on - strand, within RR42_RS32265 at 2627.746 kb on + strand, within RR42_RS32265 at 2627.747 kb on - strand, within RR42_RS32265 at 2627.747 kb on - strand, within RR42_RS32265 at 2627.822 kb on + strand, within RR42_RS32265 at 2627.822 kb on + strand, within RR42_RS32265 at 2627.823 kb on - strand, within RR42_RS32265 at 2627.823 kb on - strand, within RR42_RS32265 at 2627.848 kb on + strand, within RR42_RS32265 at 2627.848 kb on + strand, within RR42_RS32265 at 2627.848 kb on + strand, within RR42_RS32265 at 2627.848 kb on + strand, within RR42_RS32265 at 2627.849 kb on - strand, within RR42_RS32265 at 2627.849 kb on - strand, within RR42_RS32265 at 2627.849 kb on - strand, within RR42_RS32265 at 2627.900 kb on + strand at 2627.977 kb on + strand at 2627.977 kb on + strand at 2627.978 kb on - strand at 2627.978 kb on - strand at 2628.021 kb on - strand at 2628.021 kb on - strand at 2628.021 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.5 mM remove 2,624,090 - RR42_RS32250 0.74 -0.5 2,624,090 - RR42_RS32250 0.74 -0.7 2,624,090 - RR42_RS32250 0.74 -0.7 2,624,090 - RR42_RS32250 0.74 +0.7 2,624,090 - RR42_RS32250 0.74 -1.6 2,624,093 + RR42_RS32250 0.75 -1.6 2,624,093 + RR42_RS32250 0.75 +0.2 2,624,093 + RR42_RS32250 0.75 -2.0 2,624,093 + RR42_RS32250 0.75 -1.3 2,624,093 + RR42_RS32250 0.75 -1.3 2,624,093 + RR42_RS32250 0.75 -1.9 2,624,093 + RR42_RS32250 0.75 -1.4 2,624,094 - RR42_RS32250 0.75 -1.3 2,624,114 + RR42_RS32250 0.77 -0.1 2,624,115 - RR42_RS32250 0.77 -0.8 2,624,132 + RR42_RS32250 0.79 -0.3 2,624,132 + RR42_RS32250 0.79 -1.5 2,624,133 - RR42_RS32250 0.80 -0.9 2,625,121 + -0.1 2,625,199 + -0.8 2,625,200 - -1.4 2,625,200 - -0.7 2,625,301 + RR42_RS32260 0.14 +0.5 2,625,301 + RR42_RS32260 0.14 -1.9 2,625,302 - RR42_RS32260 0.14 -0.7 2,625,322 + RR42_RS32260 0.15 -1.7 2,625,457 + RR42_RS32260 0.22 +0.6 2,625,457 + RR42_RS32260 0.22 -1.4 2,625,463 + RR42_RS32260 0.22 -0.6 2,625,463 + RR42_RS32260 0.22 -1.7 2,625,463 + RR42_RS32260 0.22 -2.0 2,625,463 + RR42_RS32260 0.22 -1.4 2,625,463 + RR42_RS32260 0.22 -0.4 2,625,463 + RR42_RS32260 0.22 -0.0 2,625,464 - RR42_RS32260 0.22 -2.2 2,625,464 - RR42_RS32260 0.22 -2.2 2,625,464 - RR42_RS32260 0.22 -0.9 2,625,464 - RR42_RS32260 0.22 -1.5 2,625,484 + RR42_RS32260 0.23 -0.9 2,625,484 + RR42_RS32260 0.23 -0.4 2,625,650 + RR42_RS32260 0.31 -1.2 2,625,662 - RR42_RS32260 0.32 -1.3 2,625,662 - RR42_RS32260 0.32 -1.2 2,625,662 - RR42_RS32260 0.32 -0.6 2,625,662 - RR42_RS32260 0.32 -0.3 2,625,662 - RR42_RS32260 0.32 -0.7 2,625,878 - RR42_RS32260 0.43 -1.8 2,626,287 + RR42_RS32260 0.63 -3.1 2,626,492 + RR42_RS32260 0.73 -2.2 2,626,492 + -0.3 2,626,492 + RR42_RS32260 0.73 -2.3 2,626,493 - RR42_RS32260 0.73 -0.5 2,626,493 - RR42_RS32260 0.73 -1.9 2,626,493 - RR42_RS32260 0.73 -1.0 2,626,493 - RR42_RS32260 0.73 -1.0 2,626,608 - RR42_RS32260 0.79 -0.8 2,626,608 - RR42_RS32260 0.79 -1.7 2,626,711 + RR42_RS32260 0.84 -0.7 2,626,711 + RR42_RS32260 0.84 -1.1 2,626,712 - RR42_RS32260 0.84 -1.3 2,626,806 - RR42_RS32260 0.89 -1.7 2,626,843 + -0.6 2,626,844 - -1.8 2,626,844 - -1.0 2,626,861 + -0.5 2,626,861 + -0.7 2,627,016 + +0.1 2,627,066 + -2.4 2,627,067 - -0.0 2,627,157 + -0.7 2,627,240 - RR42_RS32265 0.13 +0.2 2,627,314 + RR42_RS32265 0.22 +1.1 2,627,315 - RR42_RS32265 0.22 +0.2 2,627,317 + RR42_RS32265 0.22 -1.8 2,627,317 + RR42_RS32265 0.22 -2.0 2,627,318 - RR42_RS32265 0.22 -0.1 2,627,434 + RR42_RS32265 0.36 -1.0 2,627,435 - RR42_RS32265 0.36 -1.8 2,627,435 - RR42_RS32265 0.36 -0.2 2,627,521 - RR42_RS32265 0.46 -0.1 2,627,593 + RR42_RS32265 0.55 -1.7 2,627,593 + RR42_RS32265 0.55 -0.6 2,627,594 - RR42_RS32265 0.55 +0.3 2,627,594 - RR42_RS32265 0.55 +1.3 2,627,594 - RR42_RS32265 0.55 -0.2 2,627,601 + RR42_RS32265 0.56 -1.5 2,627,601 + RR42_RS32265 0.56 -2.1 2,627,601 + RR42_RS32265 0.56 -1.0 2,627,602 - RR42_RS32265 0.56 -0.4 2,627,607 - RR42_RS32265 0.57 +0.7 2,627,746 + RR42_RS32265 0.73 -0.1 2,627,747 - RR42_RS32265 0.73 -0.0 2,627,747 - RR42_RS32265 0.73 +0.6 2,627,822 + RR42_RS32265 0.82 +0.7 2,627,822 + RR42_RS32265 0.82 -0.2 2,627,823 - RR42_RS32265 0.82 +0.9 2,627,823 - RR42_RS32265 0.82 +0.7 2,627,848 + RR42_RS32265 0.85 -0.2 2,627,848 + RR42_RS32265 0.85 +0.6 2,627,848 + RR42_RS32265 0.85 -0.3 2,627,848 + RR42_RS32265 0.85 -0.6 2,627,849 - RR42_RS32265 0.85 -0.1 2,627,849 - RR42_RS32265 0.85 -0.0 2,627,849 - RR42_RS32265 0.85 +0.4 2,627,900 + -0.7 2,627,977 + +1.0 2,627,977 + -0.2 2,627,978 - -0.7 2,627,978 - +0.0 2,628,021 - -0.5 2,628,021 - +1.2 2,628,021 - +0.5
Or see this region's nucleotide sequence