Experiment: R2A with Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31115 and RR42_RS31120 are separated by 93 nucleotides RR42_RS31120 and RR42_RS31125 are separated by 414 nucleotides RR42_RS31125 and RR42_RS31130 overlap by 22 nucleotides
RR42_RS31115: RR42_RS31115 - ABC transporter substrate-binding protein, at 2,360,587 to 2,361,564
_RS31115
RR42_RS31120: RR42_RS31120 - cupin, at 2,361,658 to 2,362,056
_RS31120
RR42_RS31125: RR42_RS31125 - hypothetical protein, at 2,362,471 to 2,362,740
_RS31125
RR42_RS31130: RR42_RS31130 - hypothetical protein, at 2,362,719 to 2,364,290
_RS31130
Position (kb)
2361
2362
2363 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2360.699 kb on + strand, within RR42_RS31115 at 2360.699 kb on + strand, within RR42_RS31115 at 2360.699 kb on + strand, within RR42_RS31115 at 2360.700 kb on - strand, within RR42_RS31115 at 2360.700 kb on - strand, within RR42_RS31115 at 2361.248 kb on + strand, within RR42_RS31115 at 2361.248 kb on + strand, within RR42_RS31115 at 2361.249 kb on - strand, within RR42_RS31115 at 2361.249 kb on - strand, within RR42_RS31115 at 2361.297 kb on + strand, within RR42_RS31115 at 2361.297 kb on + strand, within RR42_RS31115 at 2361.297 kb on + strand, within RR42_RS31115 at 2361.297 kb on + strand, within RR42_RS31115 at 2361.298 kb on - strand, within RR42_RS31115 at 2361.298 kb on - strand, within RR42_RS31115 at 2361.482 kb on + strand at 2361.575 kb on + strand at 2361.707 kb on + strand, within RR42_RS31120 at 2361.707 kb on + strand, within RR42_RS31120 at 2361.707 kb on + strand, within RR42_RS31120 at 2361.708 kb on - strand, within RR42_RS31120 at 2361.708 kb on - strand, within RR42_RS31120 at 2361.848 kb on + strand, within RR42_RS31120 at 2361.848 kb on + strand, within RR42_RS31120 at 2361.848 kb on + strand, within RR42_RS31120 at 2361.848 kb on + strand, within RR42_RS31120 at 2361.849 kb on - strand, within RR42_RS31120 at 2361.849 kb on - strand, within RR42_RS31120 at 2361.849 kb on - strand, within RR42_RS31120 at 2361.849 kb on - strand, within RR42_RS31120 at 2361.849 kb on - strand, within RR42_RS31120 at 2361.849 kb on - strand, within RR42_RS31120 at 2361.849 kb on - strand, within RR42_RS31120 at 2361.849 kb on - strand, within RR42_RS31120 at 2361.849 kb on - strand, within RR42_RS31120 at 2362.028 kb on + strand at 2362.028 kb on + strand at 2362.029 kb on - strand at 2362.029 kb on - strand at 2362.029 kb on - strand at 2362.029 kb on - strand at 2362.180 kb on - strand at 2362.210 kb on + strand at 2362.210 kb on + strand at 2362.211 kb on - strand at 2362.211 kb on - strand at 2362.222 kb on + strand at 2362.284 kb on + strand at 2362.285 kb on - strand at 2362.285 kb on - strand at 2362.285 kb on - strand at 2362.332 kb on + strand at 2362.332 kb on + strand at 2362.333 kb on - strand at 2362.333 kb on - strand at 2362.351 kb on + strand at 2362.351 kb on + strand at 2362.351 kb on + strand at 2362.352 kb on - strand at 2362.603 kb on + strand, within RR42_RS31125 at 2362.603 kb on + strand, within RR42_RS31125 at 2362.604 kb on - strand, within RR42_RS31125 at 2362.613 kb on - strand, within RR42_RS31125 at 2362.613 kb on - strand, within RR42_RS31125 at 2362.775 kb on + strand at 2362.775 kb on + strand at 2362.775 kb on + strand at 2362.776 kb on - strand at 2362.776 kb on - strand at 2362.938 kb on + strand, within RR42_RS31130 at 2362.939 kb on - strand, within RR42_RS31130 at 2362.939 kb on - strand, within RR42_RS31130
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.5 mM remove 2,360,699 + RR42_RS31115 0.11 +0.5 2,360,699 + RR42_RS31115 0.11 +0.7 2,360,699 + RR42_RS31115 0.11 +0.3 2,360,700 - RR42_RS31115 0.12 -1.3 2,360,700 - RR42_RS31115 0.12 +0.4 2,361,248 + RR42_RS31115 0.68 +0.7 2,361,248 + RR42_RS31115 0.68 +0.7 2,361,249 - RR42_RS31115 0.68 +1.0 2,361,249 - RR42_RS31115 0.68 +0.6 2,361,297 + RR42_RS31115 0.73 -1.5 2,361,297 + RR42_RS31115 0.73 +0.2 2,361,297 + RR42_RS31115 0.73 -0.4 2,361,297 + RR42_RS31115 0.73 +2.3 2,361,298 - RR42_RS31115 0.73 -1.3 2,361,298 - RR42_RS31115 0.73 +0.2 2,361,482 + +0.6 2,361,575 + +0.1 2,361,707 + RR42_RS31120 0.12 +0.1 2,361,707 + RR42_RS31120 0.12 +0.4 2,361,707 + RR42_RS31120 0.12 -0.4 2,361,708 - RR42_RS31120 0.13 +1.3 2,361,708 - RR42_RS31120 0.13 +0.2 2,361,848 + RR42_RS31120 0.48 +0.2 2,361,848 + RR42_RS31120 0.48 -0.6 2,361,848 + RR42_RS31120 0.48 +0.1 2,361,848 + RR42_RS31120 0.48 +0.5 2,361,849 - RR42_RS31120 0.48 -0.3 2,361,849 - RR42_RS31120 0.48 +0.6 2,361,849 - RR42_RS31120 0.48 -1.6 2,361,849 - RR42_RS31120 0.48 +0.7 2,361,849 - RR42_RS31120 0.48 -0.5 2,361,849 - RR42_RS31120 0.48 +1.9 2,361,849 - RR42_RS31120 0.48 -0.6 2,361,849 - RR42_RS31120 0.48 +0.2 2,361,849 - RR42_RS31120 0.48 +1.2 2,362,028 + -0.7 2,362,028 + +0.9 2,362,029 - -1.2 2,362,029 - -0.1 2,362,029 - +1.3 2,362,029 - -0.2 2,362,180 - +0.2 2,362,210 + -0.3 2,362,210 + +0.2 2,362,211 - -0.3 2,362,211 - +0.8 2,362,222 + -0.9 2,362,284 + -0.3 2,362,285 - -0.2 2,362,285 - +1.6 2,362,285 - -0.3 2,362,332 + -0.7 2,362,332 + -1.9 2,362,333 - +0.8 2,362,333 - +0.7 2,362,351 + +0.7 2,362,351 + +0.8 2,362,351 + -2.3 2,362,352 - -0.4 2,362,603 + RR42_RS31125 0.49 -0.3 2,362,603 + RR42_RS31125 0.49 +0.3 2,362,604 - RR42_RS31125 0.49 +0.2 2,362,613 - RR42_RS31125 0.53 +0.1 2,362,613 - RR42_RS31125 0.53 -0.4 2,362,775 + -0.0 2,362,775 + +0.2 2,362,775 + +0.9 2,362,776 - +0.4 2,362,776 - +0.4 2,362,938 + RR42_RS31130 0.14 +0.7 2,362,939 - RR42_RS31130 0.14 -0.1 2,362,939 - RR42_RS31130 0.14 +0.2
Or see this region's nucleotide sequence