Experiment: R2A with Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31060 and RR42_RS31065 are separated by 250 nucleotides RR42_RS31065 and RR42_RS31070 are separated by 58 nucleotides RR42_RS31070 and RR42_RS31075 are separated by 323 nucleotides
RR42_RS31060: RR42_RS31060 - diguanylate cyclase, at 2,348,177 to 2,349,748
_RS31060
RR42_RS31065: RR42_RS31065 - GntR family transcriptional regulator, at 2,349,999 to 2,350,673
_RS31065
RR42_RS31070: RR42_RS31070 - dihydroxy-acid dehydratase, at 2,350,732 to 2,352,462
_RS31070
RR42_RS31075: RR42_RS31075 - MFS transporter, at 2,352,786 to 2,353,814
_RS31075
Position (kb)
2350
2351
2352
2353 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2349.740 kb on + strand at 2349.741 kb on - strand at 2349.741 kb on - strand at 2349.741 kb on - strand at 2349.775 kb on + strand at 2349.775 kb on + strand at 2349.988 kb on - strand at 2349.988 kb on - strand at 2349.997 kb on + strand at 2349.997 kb on + strand at 2349.998 kb on - strand at 2350.000 kb on + strand at 2350.000 kb on + strand at 2350.039 kb on + strand at 2350.039 kb on + strand at 2350.039 kb on + strand at 2350.040 kb on - strand at 2350.074 kb on + strand, within RR42_RS31065 at 2350.075 kb on - strand, within RR42_RS31065 at 2350.204 kb on + strand at 2350.421 kb on - strand, within RR42_RS31065 at 2350.661 kb on + strand at 2350.661 kb on + strand at 2350.676 kb on + strand at 2350.676 kb on + strand at 2350.676 kb on + strand at 2350.836 kb on - strand at 2351.105 kb on + strand, within RR42_RS31070 at 2351.106 kb on - strand, within RR42_RS31070 at 2351.513 kb on + strand, within RR42_RS31070 at 2351.513 kb on + strand, within RR42_RS31070 at 2351.513 kb on + strand, within RR42_RS31070 at 2351.513 kb on + strand, within RR42_RS31070 at 2351.513 kb on + strand, within RR42_RS31070 at 2351.514 kb on - strand, within RR42_RS31070 at 2351.514 kb on - strand, within RR42_RS31070 at 2352.170 kb on + strand, within RR42_RS31070 at 2352.170 kb on + strand, within RR42_RS31070 at 2352.170 kb on + strand, within RR42_RS31070 at 2352.170 kb on + strand, within RR42_RS31070 at 2352.170 kb on + strand, within RR42_RS31070 at 2352.170 kb on + strand, within RR42_RS31070 at 2352.170 kb on + strand, within RR42_RS31070 at 2352.171 kb on + strand, within RR42_RS31070 at 2352.171 kb on - strand, within RR42_RS31070 at 2352.171 kb on - strand, within RR42_RS31070 at 2352.302 kb on + strand at 2352.303 kb on - strand at 2352.303 kb on - strand at 2352.360 kb on - strand at 2352.375 kb on + strand at 2352.376 kb on - strand at 2352.417 kb on - strand at 2352.417 kb on - strand at 2352.551 kb on - strand at 2352.685 kb on - strand at 2352.759 kb on + strand at 2352.760 kb on - strand at 2352.916 kb on - strand, within RR42_RS31075 at 2353.098 kb on + strand, within RR42_RS31075 at 2353.098 kb on + strand, within RR42_RS31075 at 2353.098 kb on + strand, within RR42_RS31075 at 2353.099 kb on - strand, within RR42_RS31075 at 2353.100 kb on + strand, within RR42_RS31075 at 2353.101 kb on - strand, within RR42_RS31075 at 2353.149 kb on + strand, within RR42_RS31075 at 2353.150 kb on - strand, within RR42_RS31075 at 2353.167 kb on + strand, within RR42_RS31075 at 2353.168 kb on - strand, within RR42_RS31075 at 2353.392 kb on + strand, within RR42_RS31075 at 2353.392 kb on + strand, within RR42_RS31075 at 2353.393 kb on - strand, within RR42_RS31075 at 2353.393 kb on - strand, within RR42_RS31075
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.5 mM remove 2,349,740 + +0.5 2,349,741 - +1.5 2,349,741 - +0.7 2,349,741 - -0.1 2,349,775 + +0.7 2,349,775 + -0.4 2,349,988 - -0.1 2,349,988 - -0.0 2,349,997 + +2.2 2,349,997 + +0.8 2,349,998 - +0.6 2,350,000 + +0.5 2,350,000 + +1.6 2,350,039 + +1.0 2,350,039 + +0.1 2,350,039 + -0.7 2,350,040 - -0.8 2,350,074 + RR42_RS31065 0.11 -0.2 2,350,075 - RR42_RS31065 0.11 -1.5 2,350,204 + +0.2 2,350,421 - RR42_RS31065 0.63 +0.7 2,350,661 + +0.5 2,350,661 + +0.0 2,350,676 + -0.7 2,350,676 + +0.2 2,350,676 + +0.0 2,350,836 - +1.0 2,351,105 + RR42_RS31070 0.22 -0.1 2,351,106 - RR42_RS31070 0.22 +0.7 2,351,513 + RR42_RS31070 0.45 +0.2 2,351,513 + RR42_RS31070 0.45 +0.1 2,351,513 + RR42_RS31070 0.45 +0.1 2,351,513 + RR42_RS31070 0.45 +0.7 2,351,513 + RR42_RS31070 0.45 -1.7 2,351,514 - RR42_RS31070 0.45 +0.5 2,351,514 - RR42_RS31070 0.45 -0.1 2,352,170 + RR42_RS31070 0.83 +0.2 2,352,170 + RR42_RS31070 0.83 -0.4 2,352,170 + RR42_RS31070 0.83 -0.4 2,352,170 + RR42_RS31070 0.83 +0.2 2,352,170 + RR42_RS31070 0.83 -0.9 2,352,170 + RR42_RS31070 0.83 -0.6 2,352,170 + RR42_RS31070 0.83 +1.7 2,352,171 + RR42_RS31070 0.83 -0.7 2,352,171 - RR42_RS31070 0.83 +0.4 2,352,171 - RR42_RS31070 0.83 +0.9 2,352,302 + -0.4 2,352,303 - -0.8 2,352,303 - +0.5 2,352,360 - +0.4 2,352,375 + -0.0 2,352,376 - +0.0 2,352,417 - +1.3 2,352,417 - -2.7 2,352,551 - -1.4 2,352,685 - -0.5 2,352,759 + +0.3 2,352,760 - -0.0 2,352,916 - RR42_RS31075 0.13 +0.9 2,353,098 + RR42_RS31075 0.30 +0.4 2,353,098 + RR42_RS31075 0.30 -0.8 2,353,098 + RR42_RS31075 0.30 +0.7 2,353,099 - RR42_RS31075 0.30 -0.7 2,353,100 + RR42_RS31075 0.31 -0.6 2,353,101 - RR42_RS31075 0.31 +0.2 2,353,149 + RR42_RS31075 0.35 +0.7 2,353,150 - RR42_RS31075 0.35 -1.6 2,353,167 + RR42_RS31075 0.37 -0.4 2,353,168 - RR42_RS31075 0.37 +1.2 2,353,392 + RR42_RS31075 0.59 -0.1 2,353,392 + RR42_RS31075 0.59 +0.7 2,353,393 - RR42_RS31075 0.59 +0.2 2,353,393 - RR42_RS31075 0.59 +2.6
Or see this region's nucleotide sequence