Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS28115

Experiment: R2A with Nickel (II) chloride 0.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS28105 and RR42_RS28110 are separated by 32 nucleotidesRR42_RS28110 and RR42_RS28115 are separated by 126 nucleotidesRR42_RS28115 and RR42_RS28120 are separated by 53 nucleotidesRR42_RS28120 and RR42_RS28125 are separated by 57 nucleotides RR42_RS28105: RR42_RS28105 - phosphonate C-P lyase system protein PhnK, at 1,664,507 to 1,665,295 _RS28105 RR42_RS28110: RR42_RS28110 - phosphonate ABC transporter ATPase, at 1,665,328 to 1,666,017 _RS28110 RR42_RS28115: RR42_RS28115 - phosphonate metabolism protein PhnM, at 1,666,144 to 1,667,385 _RS28115 RR42_RS28120: RR42_RS28120 - molybdenum cofactor biosysynthesis protein, at 1,667,439 to 1,667,969 _RS28120 RR42_RS28125: RR42_RS28125 - hypothetical protein, at 1,668,027 to 1,668,299 _RS28125 Position (kb) 1666 1667 1668Strain fitness (log2 ratio) -1 0 1 2at 1665.313 kb on + strandat 1665.508 kb on + strand, within RR42_RS28110at 1665.509 kb on - strand, within RR42_RS28110at 1665.517 kb on + strand, within RR42_RS28110at 1665.517 kb on + strand, within RR42_RS28110at 1665.517 kb on + strand, within RR42_RS28110at 1665.626 kb on - strand, within RR42_RS28110at 1665.638 kb on + strand, within RR42_RS28110at 1666.157 kb on - strandat 1666.442 kb on - strand, within RR42_RS28115at 1666.691 kb on - strand, within RR42_RS28115at 1666.699 kb on + strand, within RR42_RS28115at 1666.700 kb on - strand, within RR42_RS28115at 1666.863 kb on + strand, within RR42_RS28115at 1666.864 kb on - strand, within RR42_RS28115at 1666.958 kb on + strand, within RR42_RS28115at 1666.958 kb on + strand, within RR42_RS28115at 1666.958 kb on + strand, within RR42_RS28115at 1666.958 kb on + strand, within RR42_RS28115at 1666.958 kb on + strand, within RR42_RS28115at 1666.959 kb on - strand, within RR42_RS28115at 1667.060 kb on - strand, within RR42_RS28115at 1667.256 kb on + strand, within RR42_RS28115at 1667.256 kb on + strand, within RR42_RS28115at 1667.256 kb on + strand, within RR42_RS28115at 1667.257 kb on - strand, within RR42_RS28115at 1667.450 kb on - strandat 1667.614 kb on + strand, within RR42_RS28120at 1668.028 kb on + strandat 1668.028 kb on + strandat 1668.082 kb on - strand, within RR42_RS28125at 1668.205 kb on + strand, within RR42_RS28125at 1668.206 kb on - strand, within RR42_RS28125at 1668.259 kb on - strand, within RR42_RS28125at 1668.282 kb on + strandat 1668.282 kb on + strandat 1668.283 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.5 mM
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1,665,313 + -1.4
1,665,508 + RR42_RS28110 0.26 -0.6
1,665,509 - RR42_RS28110 0.26 +0.7
1,665,517 + RR42_RS28110 0.27 +1.1
1,665,517 + RR42_RS28110 0.27 +0.4
1,665,517 + RR42_RS28110 0.27 +0.3
1,665,626 - RR42_RS28110 0.43 +2.0
1,665,638 + RR42_RS28110 0.45 +1.5
1,666,157 - +0.6
1,666,442 - RR42_RS28115 0.24 +2.6
1,666,691 - RR42_RS28115 0.44 -0.6
1,666,699 + RR42_RS28115 0.45 +0.7
1,666,700 - RR42_RS28115 0.45 +0.0
1,666,863 + RR42_RS28115 0.58 -0.1
1,666,864 - RR42_RS28115 0.58 -0.3
1,666,958 + RR42_RS28115 0.66 -0.0
1,666,958 + RR42_RS28115 0.66 -0.3
1,666,958 + RR42_RS28115 0.66 +0.8
1,666,958 + RR42_RS28115 0.66 +0.4
1,666,958 + RR42_RS28115 0.66 +0.4
1,666,959 - RR42_RS28115 0.66 -0.3
1,667,060 - RR42_RS28115 0.74 +0.5
1,667,256 + RR42_RS28115 0.90 -0.4
1,667,256 + RR42_RS28115 0.90 +0.3
1,667,256 + RR42_RS28115 0.90 -0.9
1,667,257 - RR42_RS28115 0.90 -0.7
1,667,450 - +0.1
1,667,614 + RR42_RS28120 0.33 +0.2
1,668,028 + -0.4
1,668,028 + -0.7
1,668,082 - RR42_RS28125 0.20 +0.4
1,668,205 + RR42_RS28125 0.65 -0.6
1,668,206 - RR42_RS28125 0.66 -0.1
1,668,259 - RR42_RS28125 0.85 -0.4
1,668,282 + +0.4
1,668,282 + -0.3
1,668,283 - +0.5

Or see this region's nucleotide sequence