Experiment: R2A with Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22895 and RR42_RS22900 overlap by 4 nucleotides RR42_RS22900 and RR42_RS22905 are separated by 81 nucleotides
RR42_RS22895: RR42_RS22895 - 5-oxoprolinase, at 467,955 to 470,084
_RS22895
RR42_RS22900: RR42_RS22900 - 5-oxoprolinase, at 470,081 to 471,814
_RS22900
RR42_RS22905: RR42_RS22905 - myristoyl transferase, at 471,896 to 472,885
_RS22905
Position (kb) 470
471
472 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7 at 469.722 kb on - strand, within RR42_RS22895 at 469.722 kb on - strand, within RR42_RS22895 at 469.809 kb on + strand, within RR42_RS22895 at 470.414 kb on + strand, within RR42_RS22900 at 470.513 kb on + strand, within RR42_RS22900 at 470.550 kb on - strand, within RR42_RS22900 at 470.622 kb on + strand, within RR42_RS22900 at 470.623 kb on - strand, within RR42_RS22900 at 470.642 kb on + strand, within RR42_RS22900 at 470.673 kb on + strand, within RR42_RS22900 at 470.673 kb on + strand, within RR42_RS22900 at 470.674 kb on - strand, within RR42_RS22900 at 470.804 kb on + strand, within RR42_RS22900 at 470.804 kb on + strand, within RR42_RS22900 at 470.805 kb on - strand, within RR42_RS22900 at 470.874 kb on - strand, within RR42_RS22900 at 470.888 kb on + strand, within RR42_RS22900 at 470.939 kb on + strand, within RR42_RS22900 at 470.940 kb on - strand, within RR42_RS22900 at 470.957 kb on + strand, within RR42_RS22900 at 470.957 kb on + strand, within RR42_RS22900 at 470.958 kb on - strand, within RR42_RS22900 at 470.958 kb on - strand, within RR42_RS22900 at 471.221 kb on + strand, within RR42_RS22900 at 471.221 kb on + strand, within RR42_RS22900 at 471.221 kb on + strand, within RR42_RS22900 at 471.221 kb on + strand, within RR42_RS22900 at 471.221 kb on + strand, within RR42_RS22900 at 471.222 kb on - strand, within RR42_RS22900 at 471.365 kb on + strand, within RR42_RS22900 at 471.366 kb on - strand, within RR42_RS22900 at 471.616 kb on + strand, within RR42_RS22900 at 471.725 kb on + strand at 471.725 kb on + strand at 471.725 kb on + strand at 471.816 kb on - strand at 472.001 kb on + strand, within RR42_RS22905 at 472.378 kb on + strand, within RR42_RS22905 at 472.535 kb on + strand, within RR42_RS22905 at 472.628 kb on + strand, within RR42_RS22905 at 472.629 kb on - strand, within RR42_RS22905 at 472.629 kb on - strand, within RR42_RS22905 at 472.629 kb on - strand, within RR42_RS22905 at 472.724 kb on + strand, within RR42_RS22905 at 472.725 kb on - strand, within RR42_RS22905
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.5 mM remove 469,722 - RR42_RS22895 0.83 -0.0 469,722 - RR42_RS22895 0.83 +0.3 469,809 + RR42_RS22895 0.87 +6.4 470,414 + RR42_RS22900 0.19 +3.8 470,513 + RR42_RS22900 0.25 +4.2 470,550 - RR42_RS22900 0.27 +1.3 470,622 + RR42_RS22900 0.31 +1.9 470,623 - RR42_RS22900 0.31 +2.1 470,642 + RR42_RS22900 0.32 +3.8 470,673 + RR42_RS22900 0.34 +3.2 470,673 + RR42_RS22900 0.34 +3.2 470,674 - RR42_RS22900 0.34 +1.2 470,804 + RR42_RS22900 0.42 +5.0 470,804 + RR42_RS22900 0.42 +4.8 470,805 - RR42_RS22900 0.42 -3.3 470,874 - RR42_RS22900 0.46 +1.1 470,888 + RR42_RS22900 0.47 +5.1 470,939 + RR42_RS22900 0.49 +4.8 470,940 - RR42_RS22900 0.50 +1.5 470,957 + RR42_RS22900 0.51 +3.5 470,957 + RR42_RS22900 0.51 +4.4 470,958 - RR42_RS22900 0.51 +0.9 470,958 - RR42_RS22900 0.51 +1.1 471,221 + RR42_RS22900 0.66 +5.7 471,221 + RR42_RS22900 0.66 +5.8 471,221 + RR42_RS22900 0.66 +5.5 471,221 + RR42_RS22900 0.66 +6.2 471,221 + RR42_RS22900 0.66 +6.2 471,222 - RR42_RS22900 0.66 +2.1 471,365 + RR42_RS22900 0.74 +6.0 471,366 - RR42_RS22900 0.74 +1.9 471,616 + RR42_RS22900 0.89 +7.0 471,725 + +7.5 471,725 + +5.3 471,725 + +7.0 471,816 - +1.6 472,001 + RR42_RS22905 0.11 +3.9 472,378 + RR42_RS22905 0.49 +3.7 472,535 + RR42_RS22905 0.65 +6.3 472,628 + RR42_RS22905 0.74 +5.2 472,629 - RR42_RS22905 0.74 +2.2 472,629 - RR42_RS22905 0.74 +1.8 472,629 - RR42_RS22905 0.74 +3.2 472,724 + RR42_RS22905 0.84 +5.2 472,725 - RR42_RS22905 0.84 +3.6
Or see this region's nucleotide sequence