Experiment: R2A with Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22775 and RR42_RS22780 are separated by 20 nucleotides RR42_RS22780 and RR42_RS22785 are separated by 160 nucleotides RR42_RS22785 and RR42_RS22790 are separated by 138 nucleotides
RR42_RS22775: RR42_RS22775 - 3-isopropylmalate dehydratase, at 442,544 to 443,170
_RS22775
RR42_RS22780: RR42_RS22780 - hypothetical protein, at 443,191 to 443,745
_RS22780
RR42_RS22785: RR42_RS22785 - receptor, at 443,906 to 444,859
_RS22785
RR42_RS22790: RR42_RS22790 - MmgE/PrpD family protein, at 444,998 to 446,410
_RS22790
Position (kb) 443
444
445 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 443.828 kb on - strand at 443.840 kb on + strand at 444.008 kb on + strand, within RR42_RS22785 at 444.011 kb on - strand, within RR42_RS22785 at 444.026 kb on - strand, within RR42_RS22785 at 444.160 kb on + strand, within RR42_RS22785 at 444.160 kb on + strand, within RR42_RS22785 at 444.161 kb on - strand, within RR42_RS22785 at 444.200 kb on + strand, within RR42_RS22785 at 444.201 kb on - strand, within RR42_RS22785 at 444.279 kb on + strand, within RR42_RS22785 at 444.279 kb on + strand, within RR42_RS22785 at 444.280 kb on - strand, within RR42_RS22785 at 444.329 kb on + strand, within RR42_RS22785 at 444.444 kb on - strand, within RR42_RS22785 at 444.444 kb on - strand, within RR42_RS22785 at 444.530 kb on + strand, within RR42_RS22785 at 444.601 kb on + strand, within RR42_RS22785 at 444.804 kb on + strand at 444.804 kb on + strand at 444.846 kb on - strand at 444.908 kb on + strand at 444.909 kb on - strand at 444.918 kb on + strand at 444.918 kb on + strand at 444.918 kb on + strand at 445.292 kb on + strand, within RR42_RS22790 at 445.293 kb on - strand, within RR42_RS22790 at 445.410 kb on - strand, within RR42_RS22790 at 445.604 kb on + strand, within RR42_RS22790
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.5 mM remove 443,828 - +0.9 443,840 + +3.3 444,008 + RR42_RS22785 0.11 +0.1 444,011 - RR42_RS22785 0.11 +0.7 444,026 - RR42_RS22785 0.13 -2.0 444,160 + RR42_RS22785 0.27 -1.0 444,160 + RR42_RS22785 0.27 +0.5 444,161 - RR42_RS22785 0.27 +1.2 444,200 + RR42_RS22785 0.31 -0.3 444,201 - RR42_RS22785 0.31 +1.9 444,279 + RR42_RS22785 0.39 +1.1 444,279 + RR42_RS22785 0.39 +0.5 444,280 - RR42_RS22785 0.39 -0.5 444,329 + RR42_RS22785 0.44 +0.8 444,444 - RR42_RS22785 0.56 +1.4 444,444 - RR42_RS22785 0.56 -1.1 444,530 + RR42_RS22785 0.65 +0.5 444,601 + RR42_RS22785 0.73 +0.4 444,804 + +0.6 444,804 + +0.3 444,846 - -2.0 444,908 + -0.7 444,909 - +0.5 444,918 + +0.4 444,918 + +1.8 444,918 + +1.1 445,292 + RR42_RS22790 0.21 -0.3 445,293 - RR42_RS22790 0.21 -0.8 445,410 - RR42_RS22790 0.29 -1.7 445,604 + RR42_RS22790 0.43 +1.1
Or see this region's nucleotide sequence