Experiment: R2A with Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22035 and RR42_RS22040 are separated by 100 nucleotides RR42_RS22040 and RR42_RS22045 are separated by 15 nucleotides RR42_RS22045 and RR42_RS22050 are separated by 11 nucleotides RR42_RS22050 and RR42_RS22055 overlap by 4 nucleotides
RR42_RS22035: RR42_RS22035 - ATPase, at 275,686 to 278,376
_RS22035
RR42_RS22040: RR42_RS22040 - Hcp family T6SS protein CtsH1, at 278,477 to 278,962
_RS22040
RR42_RS22045: RR42_RS22045 - flagellar motor protein MotB, at 278,978 to 280,693
_RS22045
RR42_RS22050: RR42_RS22050 - DotU family type IV / VI secretion system protein, at 280,705 to 281,358
_RS22050
RR42_RS22055: RR42_RS22055 - type VI secretion protein, at 281,355 to 282,662
_RS22055
Position (kb) 278
279
280
281 Strain fitness (log2 ratio) -2
-1
0
1 at 278.198 kb on + strand at 278.422 kb on + strand at 278.527 kb on - strand, within RR42_RS22040 at 278.695 kb on - strand, within RR42_RS22040 at 278.746 kb on - strand, within RR42_RS22040 at 278.746 kb on - strand, within RR42_RS22040 at 278.752 kb on - strand, within RR42_RS22040 at 278.752 kb on - strand, within RR42_RS22040 at 278.946 kb on + strand at 278.946 kb on + strand at 278.946 kb on + strand at 278.946 kb on + strand at 278.947 kb on - strand at 278.947 kb on - strand at 278.947 kb on - strand at 278.947 kb on - strand at 278.947 kb on - strand at 279.127 kb on - strand at 279.127 kb on - strand at 279.456 kb on + strand, within RR42_RS22045 at 279.592 kb on + strand, within RR42_RS22045 at 279.825 kb on + strand, within RR42_RS22045 at 279.825 kb on + strand, within RR42_RS22045 at 280.068 kb on - strand, within RR42_RS22045 at 280.068 kb on - strand, within RR42_RS22045 at 280.448 kb on - strand, within RR42_RS22045 at 280.459 kb on + strand, within RR42_RS22045 at 280.976 kb on + strand, within RR42_RS22050 at 281.388 kb on + strand at 281.389 kb on - strand at 281.389 kb on - strand at 281.413 kb on + strand at 281.413 kb on + strand at 281.414 kb on - strand at 281.479 kb on + strand at 281.594 kb on + strand, within RR42_RS22055 at 281.595 kb on - strand, within RR42_RS22055 at 281.595 kb on - strand, within RR42_RS22055 at 281.659 kb on + strand, within RR42_RS22055
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.5 mM remove 278,198 + -1.7 278,422 + +0.4 278,527 - RR42_RS22040 0.10 -0.5 278,695 - RR42_RS22040 0.45 -0.1 278,746 - RR42_RS22040 0.55 -0.4 278,746 - RR42_RS22040 0.55 -0.1 278,752 - RR42_RS22040 0.57 -0.3 278,752 - RR42_RS22040 0.57 +1.1 278,946 + +1.4 278,946 + -1.6 278,946 + +0.2 278,946 + +0.2 278,947 - +0.9 278,947 - +0.0 278,947 - -0.4 278,947 - -0.9 278,947 - +0.5 279,127 - -0.2 279,127 - -0.0 279,456 + RR42_RS22045 0.28 -2.4 279,592 + RR42_RS22045 0.36 +0.1 279,825 + RR42_RS22045 0.49 +1.2 279,825 + RR42_RS22045 0.49 -2.2 280,068 - RR42_RS22045 0.64 -2.6 280,068 - RR42_RS22045 0.64 -0.4 280,448 - RR42_RS22045 0.86 -0.2 280,459 + RR42_RS22045 0.86 +0.9 280,976 + RR42_RS22050 0.41 -0.2 281,388 + -1.6 281,389 - +1.6 281,389 - +0.7 281,413 + -1.6 281,413 + +0.5 281,414 - +0.1 281,479 + -0.4 281,594 + RR42_RS22055 0.18 -0.5 281,595 - RR42_RS22055 0.18 -1.0 281,595 - RR42_RS22055 0.18 -0.5 281,659 + RR42_RS22055 0.23 +0.4
Or see this region's nucleotide sequence