Experiment: R2A with Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21820 and RR42_RS21825 are separated by 89 nucleotides RR42_RS21825 and RR42_RS21830 are separated by 96 nucleotides RR42_RS21830 and RR42_RS21835 are separated by 116 nucleotides
RR42_RS21820: RR42_RS21820 - GntR family transcriptional regulator, at 224,526 to 225,230
_RS21820
RR42_RS21825: RR42_RS21825 - hypothetical protein, at 225,320 to 225,868
_RS21825
RR42_RS21830: RR42_RS21830 - transcriptional regulator, at 225,965 to 226,879
_RS21830
RR42_RS21835: RR42_RS21835 - muconate cycloisomerase, at 226,996 to 228,117
_RS21835
Position (kb) 225
226
227 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 225.072 kb on - strand, within RR42_RS21820 at 225.072 kb on - strand, within RR42_RS21820 at 225.129 kb on + strand, within RR42_RS21820 at 225.216 kb on + strand at 225.216 kb on + strand at 225.216 kb on + strand at 225.217 kb on - strand at 225.217 kb on - strand at 225.296 kb on + strand at 225.321 kb on + strand at 225.322 kb on - strand at 225.584 kb on - strand, within RR42_RS21825 at 225.709 kb on + strand, within RR42_RS21825 at 225.710 kb on - strand, within RR42_RS21825 at 225.710 kb on - strand, within RR42_RS21825 at 225.897 kb on + strand at 225.897 kb on + strand at 226.040 kb on + strand at 226.066 kb on - strand, within RR42_RS21830 at 226.066 kb on - strand, within RR42_RS21830 at 226.276 kb on - strand, within RR42_RS21830 at 226.276 kb on - strand, within RR42_RS21830 at 226.491 kb on - strand, within RR42_RS21830 at 226.491 kb on - strand, within RR42_RS21830 at 226.572 kb on + strand, within RR42_RS21830 at 226.573 kb on - strand, within RR42_RS21830 at 226.801 kb on + strand at 226.802 kb on - strand at 226.857 kb on + strand at 226.857 kb on + strand at 226.857 kb on + strand at 226.871 kb on + strand at 226.871 kb on + strand at 226.872 kb on - strand at 226.872 kb on - strand at 226.872 kb on - strand at 226.872 kb on - strand at 226.920 kb on + strand at 226.921 kb on - strand at 226.921 kb on - strand at 226.921 kb on - strand at 226.955 kb on + strand at 226.956 kb on - strand at 226.956 kb on - strand at 226.956 kb on - strand at 226.956 kb on - strand at 226.971 kb on - strand at 226.971 kb on - strand at 226.977 kb on + strand at 226.977 kb on + strand at 226.978 kb on - strand at 227.167 kb on + strand, within RR42_RS21835 at 227.167 kb on + strand, within RR42_RS21835 at 227.167 kb on + strand, within RR42_RS21835 at 227.209 kb on - strand, within RR42_RS21835 at 227.212 kb on + strand, within RR42_RS21835 at 227.213 kb on - strand, within RR42_RS21835 at 227.213 kb on - strand, within RR42_RS21835
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.5 mM remove 225,072 - RR42_RS21820 0.77 +0.3 225,072 - RR42_RS21820 0.77 -1.4 225,129 + RR42_RS21820 0.86 -0.0 225,216 + +0.2 225,216 + +0.2 225,216 + +0.6 225,217 - +0.7 225,217 - +0.9 225,296 + +0.1 225,321 + +1.4 225,322 - +0.5 225,584 - RR42_RS21825 0.48 -0.5 225,709 + RR42_RS21825 0.71 -1.3 225,710 - RR42_RS21825 0.71 -1.6 225,710 - RR42_RS21825 0.71 +0.6 225,897 + +1.1 225,897 + +0.4 226,040 + +0.3 226,066 - RR42_RS21830 0.11 -1.4 226,066 - RR42_RS21830 0.11 -0.9 226,276 - RR42_RS21830 0.34 -3.0 226,276 - RR42_RS21830 0.34 -2.0 226,491 - RR42_RS21830 0.57 -0.4 226,491 - RR42_RS21830 0.57 -0.5 226,572 + RR42_RS21830 0.66 +0.4 226,573 - RR42_RS21830 0.66 +0.3 226,801 + +1.4 226,802 - -1.9 226,857 + -0.2 226,857 + +0.0 226,857 + -0.4 226,871 + -0.4 226,871 + -0.7 226,872 - -0.6 226,872 - -0.7 226,872 - -0.3 226,872 - +0.5 226,920 + -1.4 226,921 - -1.5 226,921 - -0.9 226,921 - +0.5 226,955 + -0.8 226,956 - +0.5 226,956 - -1.6 226,956 - -0.1 226,956 - +0.1 226,971 - +0.8 226,971 - -0.8 226,977 + -0.4 226,977 + -0.3 226,978 - -0.5 227,167 + RR42_RS21835 0.15 +0.2 227,167 + RR42_RS21835 0.15 +0.6 227,167 + RR42_RS21835 0.15 +0.3 227,209 - RR42_RS21835 0.19 -1.3 227,212 + RR42_RS21835 0.19 -0.5 227,213 - RR42_RS21835 0.19 -1.7 227,213 - RR42_RS21835 0.19 -0.4
Or see this region's nucleotide sequence