Experiment: R2A with Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21750 and RR42_RS21755 are separated by 370 nucleotides RR42_RS21755 and RR42_RS21760 are separated by 105 nucleotides
RR42_RS21750: RR42_RS21750 - carnitine dehydratase, at 207,952 to 209,109
_RS21750
RR42_RS21755: RR42_RS21755 - shikimate dehydrogenase, at 209,480 to 210,316
_RS21755
RR42_RS21760: RR42_RS21760 - succinate-semialdehyde dehydrogenase, at 210,422 to 211,870
_RS21760
Position (kb) 209
210
211 Strain fitness (log2 ratio) -2
-1
0
1
2 at 208.651 kb on + strand, within RR42_RS21750 at 208.660 kb on + strand, within RR42_RS21750 at 208.661 kb on - strand, within RR42_RS21750 at 208.661 kb on - strand, within RR42_RS21750 at 208.661 kb on - strand, within RR42_RS21750 at 208.661 kb on - strand, within RR42_RS21750 at 208.682 kb on - strand, within RR42_RS21750 at 208.682 kb on - strand, within RR42_RS21750 at 209.111 kb on - strand at 209.111 kb on - strand at 209.111 kb on - strand at 209.225 kb on + strand at 209.226 kb on - strand at 209.226 kb on - strand at 209.226 kb on - strand at 209.226 kb on - strand at 209.291 kb on + strand at 209.396 kb on - strand at 209.461 kb on + strand at 209.675 kb on + strand, within RR42_RS21755 at 209.675 kb on + strand, within RR42_RS21755 at 209.848 kb on - strand, within RR42_RS21755 at 210.445 kb on - strand at 210.445 kb on - strand at 210.459 kb on + strand at 210.459 kb on + strand at 210.460 kb on - strand at 210.460 kb on - strand at 210.571 kb on - strand, within RR42_RS21760 at 210.571 kb on - strand, within RR42_RS21760 at 210.764 kb on - strand, within RR42_RS21760 at 210.844 kb on + strand, within RR42_RS21760 at 210.987 kb on + strand, within RR42_RS21760
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.5 mM remove 208,651 + RR42_RS21750 0.60 -0.4 208,660 + RR42_RS21750 0.61 -0.1 208,661 - RR42_RS21750 0.61 -0.1 208,661 - RR42_RS21750 0.61 +0.0 208,661 - RR42_RS21750 0.61 +0.5 208,661 - RR42_RS21750 0.61 -0.3 208,682 - RR42_RS21750 0.63 +1.3 208,682 - RR42_RS21750 0.63 -0.4 209,111 - +0.9 209,111 - -0.1 209,111 - -0.5 209,225 + -0.6 209,226 - +1.0 209,226 - +0.5 209,226 - +0.3 209,226 - +0.0 209,291 + -2.0 209,396 - +0.3 209,461 + -1.4 209,675 + RR42_RS21755 0.23 +0.6 209,675 + RR42_RS21755 0.23 +0.9 209,848 - RR42_RS21755 0.44 +2.3 210,445 - -0.2 210,445 - -0.9 210,459 + +0.8 210,459 + +0.3 210,460 - -0.4 210,460 - +0.2 210,571 - RR42_RS21760 0.10 +0.5 210,571 - RR42_RS21760 0.10 +1.7 210,764 - RR42_RS21760 0.24 +0.8 210,844 + RR42_RS21760 0.29 -0.5 210,987 + RR42_RS21760 0.39 -0.7
Or see this region's nucleotide sequence