Experiment: R2A with Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21615 and RR42_RS21620 are separated by 20 nucleotides RR42_RS21620 and RR42_RS21625 are separated by 83 nucleotides RR42_RS21625 and RR42_RS21630 are separated by 381 nucleotides
RR42_RS21615: RR42_RS21615 - alcohol dehydrogenase, at 177,556 to 180,558
_RS21615
RR42_RS21620: RR42_RS21620 - ferredoxin, at 180,579 to 181,085
_RS21620
RR42_RS21625: RR42_RS21625 - 6-hydroxynicotinate 3-monooxygenase, at 181,169 to 182,323
_RS21625
RR42_RS21630: RR42_RS21630 - Asp/Glu racemase, at 182,705 to 183,457
_RS21630
Position (kb) 181
182
183 Strain fitness (log2 ratio) -2
-1
0
1
2 at 180.188 kb on + strand, within RR42_RS21615 at 180.388 kb on + strand at 180.388 kb on + strand at 180.388 kb on + strand at 180.388 kb on + strand at 180.389 kb on - strand at 180.797 kb on + strand, within RR42_RS21620 at 180.798 kb on - strand, within RR42_RS21620 at 180.958 kb on + strand, within RR42_RS21620 at 180.958 kb on + strand, within RR42_RS21620 at 180.998 kb on - strand, within RR42_RS21620 at 180.998 kb on - strand, within RR42_RS21620 at 180.998 kb on - strand, within RR42_RS21620 at 181.039 kb on + strand at 181.040 kb on - strand at 181.040 kb on - strand at 181.040 kb on - strand at 181.273 kb on + strand at 181.274 kb on - strand at 181.424 kb on - strand, within RR42_RS21625 at 181.424 kb on - strand, within RR42_RS21625 at 181.653 kb on + strand, within RR42_RS21625 at 181.654 kb on - strand, within RR42_RS21625 at 181.656 kb on + strand, within RR42_RS21625 at 181.657 kb on - strand, within RR42_RS21625 at 181.657 kb on - strand, within RR42_RS21625 at 181.687 kb on - strand, within RR42_RS21625 at 181.687 kb on - strand, within RR42_RS21625 at 181.687 kb on - strand, within RR42_RS21625 at 181.696 kb on + strand, within RR42_RS21625 at 181.697 kb on - strand, within RR42_RS21625 at 181.697 kb on - strand, within RR42_RS21625 at 181.697 kb on - strand, within RR42_RS21625 at 182.044 kb on - strand, within RR42_RS21625 at 182.226 kb on + strand at 182.227 kb on - strand at 182.500 kb on + strand at 182.500 kb on + strand at 182.500 kb on + strand at 182.501 kb on - strand at 182.501 kb on - strand at 182.501 kb on - strand at 182.975 kb on + strand, within RR42_RS21630 at 183.114 kb on - strand, within RR42_RS21630 at 183.116 kb on - strand, within RR42_RS21630
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.5 mM remove 180,188 + RR42_RS21615 0.88 -2.3 180,388 + +0.1 180,388 + -1.1 180,388 + +0.4 180,388 + +0.6 180,389 - +0.2 180,797 + RR42_RS21620 0.43 -0.5 180,798 - RR42_RS21620 0.43 +0.5 180,958 + RR42_RS21620 0.75 +2.1 180,958 + RR42_RS21620 0.75 -0.2 180,998 - RR42_RS21620 0.83 +2.2 180,998 - RR42_RS21620 0.83 +1.0 180,998 - RR42_RS21620 0.83 +0.5 181,039 + +1.9 181,040 - +0.8 181,040 - -0.4 181,040 - +0.1 181,273 + -0.3 181,274 - -0.7 181,424 - RR42_RS21625 0.22 +0.3 181,424 - RR42_RS21625 0.22 +0.9 181,653 + RR42_RS21625 0.42 +1.1 181,654 - RR42_RS21625 0.42 -2.2 181,656 + RR42_RS21625 0.42 +0.5 181,657 - RR42_RS21625 0.42 +0.5 181,657 - RR42_RS21625 0.42 +0.1 181,687 - RR42_RS21625 0.45 +0.9 181,687 - RR42_RS21625 0.45 +0.0 181,687 - RR42_RS21625 0.45 +1.1 181,696 + RR42_RS21625 0.46 -0.4 181,697 - RR42_RS21625 0.46 -0.3 181,697 - RR42_RS21625 0.46 -0.7 181,697 - RR42_RS21625 0.46 +0.4 182,044 - RR42_RS21625 0.76 +0.3 182,226 + +1.0 182,227 - -0.1 182,500 + -2.1 182,500 + -1.5 182,500 + -1.2 182,501 - +0.0 182,501 - -1.1 182,501 - -1.3 182,975 + RR42_RS21630 0.36 +0.6 183,114 - RR42_RS21630 0.54 +0.9 183,116 - RR42_RS21630 0.55 +0.0
Or see this region's nucleotide sequence