Experiment: R2A with Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20440 and RR42_RS20445 overlap by 4 nucleotides RR42_RS20445 and RR42_RS20450 are separated by 175 nucleotides RR42_RS20450 and RR42_RS20455 are separated by 232 nucleotides
RR42_RS20440: RR42_RS20440 - histidine kinase, at 4,426,814 to 4,429,282
_RS20440
RR42_RS20445: RR42_RS20445 - hypothetical protein, at 4,429,279 to 4,429,998
_RS20445
RR42_RS20450: RR42_RS20450 - 16S rRNA methyltransferase, at 4,430,174 to 4,431,526
_RS20450
RR42_RS20455: RR42_RS20455 - protease HtpX, at 4,431,759 to 4,432,619
_RS20455
Position (kb) 4430
4431
4432 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4429.234 kb on - strand at 4430.200 kb on - strand at 4430.368 kb on + strand, within RR42_RS20450 at 4430.368 kb on + strand, within RR42_RS20450 at 4430.368 kb on + strand, within RR42_RS20450 at 4430.368 kb on + strand, within RR42_RS20450 at 4430.369 kb on - strand, within RR42_RS20450 at 4430.369 kb on - strand, within RR42_RS20450 at 4430.382 kb on + strand, within RR42_RS20450 at 4430.382 kb on + strand, within RR42_RS20450 at 4430.382 kb on + strand, within RR42_RS20450 at 4430.383 kb on - strand, within RR42_RS20450 at 4430.383 kb on - strand, within RR42_RS20450 at 4430.383 kb on - strand, within RR42_RS20450 at 4430.383 kb on - strand, within RR42_RS20450 at 4430.383 kb on - strand, within RR42_RS20450 at 4430.383 kb on - strand, within RR42_RS20450 at 4430.551 kb on - strand, within RR42_RS20450 at 4430.594 kb on + strand, within RR42_RS20450 at 4430.926 kb on - strand, within RR42_RS20450 at 4431.216 kb on + strand, within RR42_RS20450 at 4431.216 kb on + strand, within RR42_RS20450 at 4431.216 kb on + strand, within RR42_RS20450 at 4431.217 kb on - strand, within RR42_RS20450 at 4431.548 kb on + strand at 4431.548 kb on + strand at 4431.549 kb on - strand at 4431.658 kb on - strand at 4431.664 kb on + strand at 4431.664 kb on + strand at 4431.664 kb on + strand at 4431.664 kb on + strand at 4431.664 kb on + strand at 4431.664 kb on + strand at 4431.665 kb on - strand at 4431.760 kb on + strand at 4431.760 kb on + strand at 4431.760 kb on + strand at 4431.760 kb on + strand at 4431.760 kb on + strand at 4431.760 kb on + strand at 4431.760 kb on + strand at 4431.760 kb on + strand at 4431.761 kb on - strand at 4431.761 kb on - strand at 4431.761 kb on - strand at 4431.761 kb on - strand at 4431.761 kb on - strand at 4431.761 kb on - strand at 4431.761 kb on - strand at 4431.770 kb on - strand at 4431.770 kb on - strand at 4431.935 kb on - strand, within RR42_RS20455 at 4432.354 kb on + strand, within RR42_RS20455 at 4432.408 kb on + strand, within RR42_RS20455 at 4432.450 kb on + strand, within RR42_RS20455 at 4432.451 kb on - strand, within RR42_RS20455 at 4432.451 kb on - strand, within RR42_RS20455 at 4432.451 kb on - strand, within RR42_RS20455 at 4432.451 kb on - strand, within RR42_RS20455 at 4432.451 kb on - strand, within RR42_RS20455 at 4432.483 kb on + strand, within RR42_RS20455 at 4432.483 kb on + strand, within RR42_RS20455 at 4432.483 kb on + strand, within RR42_RS20455 at 4432.484 kb on - strand, within RR42_RS20455 at 4432.484 kb on - strand, within RR42_RS20455 at 4432.484 kb on - strand, within RR42_RS20455
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.5 mM remove 4,429,234 - -0.2 4,430,200 - +0.4 4,430,368 + RR42_RS20450 0.14 -3.4 4,430,368 + RR42_RS20450 0.14 -0.8 4,430,368 + RR42_RS20450 0.14 -2.2 4,430,368 + RR42_RS20450 0.14 -2.1 4,430,369 - RR42_RS20450 0.14 -0.6 4,430,369 - RR42_RS20450 0.14 -0.4 4,430,382 + RR42_RS20450 0.15 -1.5 4,430,382 + RR42_RS20450 0.15 -1.8 4,430,382 + RR42_RS20450 0.15 -0.3 4,430,383 - RR42_RS20450 0.15 +0.5 4,430,383 - RR42_RS20450 0.15 -0.4 4,430,383 - RR42_RS20450 0.15 -1.1 4,430,383 - RR42_RS20450 0.15 -0.4 4,430,383 - RR42_RS20450 0.15 +0.6 4,430,383 - RR42_RS20450 0.15 +0.1 4,430,551 - RR42_RS20450 0.28 +0.2 4,430,594 + RR42_RS20450 0.31 +0.2 4,430,926 - RR42_RS20450 0.56 -1.0 4,431,216 + RR42_RS20450 0.77 -1.3 4,431,216 + RR42_RS20450 0.77 +0.3 4,431,216 + RR42_RS20450 0.77 -0.7 4,431,217 - RR42_RS20450 0.77 -0.9 4,431,548 + -0.0 4,431,548 + -0.4 4,431,549 - +1.5 4,431,658 - -0.3 4,431,664 + -0.3 4,431,664 + -0.5 4,431,664 + -0.4 4,431,664 + -0.2 4,431,664 + +1.3 4,431,664 + -0.8 4,431,665 - +0.2 4,431,760 + -0.8 4,431,760 + +0.0 4,431,760 + +0.1 4,431,760 + +0.3 4,431,760 + -0.4 4,431,760 + -0.5 4,431,760 + -0.2 4,431,760 + +0.4 4,431,761 - -0.1 4,431,761 - +0.1 4,431,761 - -1.5 4,431,761 - +0.1 4,431,761 - -0.1 4,431,761 - +0.6 4,431,761 - -0.4 4,431,770 - +1.2 4,431,770 - -0.1 4,431,935 - RR42_RS20455 0.20 +1.0 4,432,354 + RR42_RS20455 0.69 +0.5 4,432,408 + RR42_RS20455 0.75 -0.4 4,432,450 + RR42_RS20455 0.80 +0.2 4,432,451 - RR42_RS20455 0.80 -1.2 4,432,451 - RR42_RS20455 0.80 -2.0 4,432,451 - RR42_RS20455 0.80 -0.1 4,432,451 - RR42_RS20455 0.80 -1.3 4,432,451 - RR42_RS20455 0.80 -0.2 4,432,483 + RR42_RS20455 0.84 +1.3 4,432,483 + RR42_RS20455 0.84 -0.0 4,432,483 + RR42_RS20455 0.84 -3.2 4,432,484 - RR42_RS20455 0.84 -0.3 4,432,484 - RR42_RS20455 0.84 -1.2 4,432,484 - RR42_RS20455 0.84 -0.3
Or see this region's nucleotide sequence