Experiment: R2A with Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20340 and RR42_RS20345 are separated by 12 nucleotides RR42_RS20345 and RR42_RS20350 overlap by 4 nucleotides RR42_RS20350 and RR42_RS20355 overlap by 4 nucleotides
RR42_RS20340: RR42_RS20340 - LysR family transcriptional regulator, at 4,399,982 to 4,400,908
_RS20340
RR42_RS20345: RR42_RS20345 - penicillin-binding protein, at 4,400,921 to 4,403,125
_RS20345
RR42_RS20350: RR42_RS20350 - hypothetical protein, at 4,403,122 to 4,403,823
_RS20350
RR42_RS20355: RR42_RS20355 - alpha-2-macroglobulin, at 4,403,820 to 4,409,840
_RS20355
Position (kb)
4400
4401
4402
4403
4404 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 4399.968 kb on - strand at 4400.142 kb on + strand, within RR42_RS20340 at 4400.519 kb on + strand, within RR42_RS20340 at 4400.520 kb on - strand, within RR42_RS20340 at 4400.582 kb on + strand, within RR42_RS20340 at 4400.583 kb on - strand, within RR42_RS20340 at 4400.609 kb on + strand, within RR42_RS20340 at 4400.610 kb on - strand, within RR42_RS20340 at 4400.817 kb on - strand at 4400.817 kb on - strand at 4400.817 kb on - strand at 4400.817 kb on - strand at 4400.822 kb on + strand at 4400.822 kb on + strand at 4400.823 kb on - strand at 4400.823 kb on - strand at 4400.906 kb on + strand at 4400.907 kb on - strand at 4401.266 kb on - strand, within RR42_RS20345 at 4401.481 kb on - strand, within RR42_RS20345 at 4401.631 kb on - strand, within RR42_RS20345 at 4401.729 kb on + strand, within RR42_RS20345 at 4401.790 kb on - strand, within RR42_RS20345 at 4401.790 kb on - strand, within RR42_RS20345 at 4401.792 kb on + strand, within RR42_RS20345 at 4401.793 kb on - strand, within RR42_RS20345 at 4401.885 kb on - strand, within RR42_RS20345 at 4401.933 kb on + strand, within RR42_RS20345 at 4401.934 kb on - strand, within RR42_RS20345 at 4401.960 kb on + strand, within RR42_RS20345 at 4401.960 kb on + strand, within RR42_RS20345 at 4401.961 kb on - strand, within RR42_RS20345 at 4401.961 kb on - strand, within RR42_RS20345 at 4402.054 kb on - strand, within RR42_RS20345 at 4402.054 kb on - strand, within RR42_RS20345 at 4402.146 kb on + strand, within RR42_RS20345 at 4402.146 kb on + strand, within RR42_RS20345 at 4402.146 kb on + strand, within RR42_RS20345 at 4402.147 kb on - strand, within RR42_RS20345 at 4402.322 kb on + strand, within RR42_RS20345 at 4402.323 kb on - strand, within RR42_RS20345 at 4402.560 kb on + strand, within RR42_RS20345 at 4402.561 kb on - strand, within RR42_RS20345 at 4402.561 kb on - strand, within RR42_RS20345 at 4402.633 kb on - strand, within RR42_RS20345 at 4402.861 kb on - strand, within RR42_RS20345 at 4402.861 kb on - strand, within RR42_RS20345 at 4403.205 kb on + strand, within RR42_RS20350 at 4403.342 kb on + strand, within RR42_RS20350 at 4403.343 kb on - strand, within RR42_RS20350 at 4403.748 kb on - strand, within RR42_RS20350 at 4403.868 kb on + strand at 4403.868 kb on + strand at 4403.881 kb on + strand at 4403.881 kb on + strand at 4403.974 kb on + strand at 4403.974 kb on + strand at 4403.975 kb on - strand at 4403.980 kb on + strand at 4403.981 kb on - strand at 4403.981 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.5 mM remove 4,399,968 - +0.4 4,400,142 + RR42_RS20340 0.17 -0.4 4,400,519 + RR42_RS20340 0.58 -0.4 4,400,520 - RR42_RS20340 0.58 -0.2 4,400,582 + RR42_RS20340 0.65 -0.3 4,400,583 - RR42_RS20340 0.65 -2.9 4,400,609 + RR42_RS20340 0.68 +0.3 4,400,610 - RR42_RS20340 0.68 -1.3 4,400,817 - +0.2 4,400,817 - -0.2 4,400,817 - -0.3 4,400,817 - -0.6 4,400,822 + +0.0 4,400,822 + -0.1 4,400,823 - -0.8 4,400,823 - +0.2 4,400,906 + -1.4 4,400,907 - -1.0 4,401,266 - RR42_RS20345 0.16 -0.9 4,401,481 - RR42_RS20345 0.25 +0.5 4,401,631 - RR42_RS20345 0.32 +0.3 4,401,729 + RR42_RS20345 0.37 -0.6 4,401,790 - RR42_RS20345 0.39 +1.2 4,401,790 - RR42_RS20345 0.39 -2.7 4,401,792 + RR42_RS20345 0.40 +0.7 4,401,793 - RR42_RS20345 0.40 +0.6 4,401,885 - RR42_RS20345 0.44 +0.1 4,401,933 + RR42_RS20345 0.46 +0.1 4,401,934 - RR42_RS20345 0.46 +1.6 4,401,960 + RR42_RS20345 0.47 -1.6 4,401,960 + RR42_RS20345 0.47 -0.0 4,401,961 - RR42_RS20345 0.47 -0.0 4,401,961 - RR42_RS20345 0.47 +0.7 4,402,054 - RR42_RS20345 0.51 -0.6 4,402,054 - RR42_RS20345 0.51 -0.5 4,402,146 + RR42_RS20345 0.56 -0.0 4,402,146 + RR42_RS20345 0.56 +0.1 4,402,146 + RR42_RS20345 0.56 +0.2 4,402,147 - RR42_RS20345 0.56 -0.2 4,402,322 + RR42_RS20345 0.64 +0.7 4,402,323 - RR42_RS20345 0.64 -0.1 4,402,560 + RR42_RS20345 0.74 +6.5 4,402,561 - RR42_RS20345 0.74 -1.3 4,402,561 - RR42_RS20345 0.74 +0.7 4,402,633 - RR42_RS20345 0.78 -1.3 4,402,861 - RR42_RS20345 0.88 +1.7 4,402,861 - RR42_RS20345 0.88 -0.1 4,403,205 + RR42_RS20350 0.12 +1.2 4,403,342 + RR42_RS20350 0.31 -2.5 4,403,343 - RR42_RS20350 0.31 +1.3 4,403,748 - RR42_RS20350 0.89 +0.5 4,403,868 + -2.3 4,403,868 + +0.9 4,403,881 + -1.0 4,403,881 + -0.6 4,403,974 + +0.2 4,403,974 + -1.3 4,403,975 - -1.5 4,403,980 + -0.7 4,403,981 - -0.1 4,403,981 - -0.1
Or see this region's nucleotide sequence