Experiment: R2A with Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20110 and RR42_RS20115 are separated by 228 nucleotides RR42_RS20115 and RR42_RS20120 are separated by 314 nucleotides RR42_RS20120 and RR42_RS20125 are separated by 145 nucleotides
RR42_RS20110: RR42_RS20110 - hypothetical protein, at 4,345,287 to 4,347,200
_RS20110
RR42_RS20115: RR42_RS20115 - antitoxin, at 4,347,429 to 4,347,680
_RS20115
RR42_RS20120: RR42_RS20120 - amino acid ABC transporter substrate-binding protein, at 4,347,995 to 4,349,146
_RS20120
RR42_RS20125: RR42_RS20125 - L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88); Proline dehydrogenase (EC 1.5.5.2) (from data) , at 4,349,292 to 4,353,293
_RS20125
Position (kb)
4347
4348
4349
4350 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4347.159 kb on + strand at 4347.179 kb on - strand at 4347.179 kb on - strand at 4347.238 kb on + strand at 4347.238 kb on + strand at 4347.238 kb on + strand at 4347.239 kb on - strand at 4347.239 kb on - strand at 4347.294 kb on - strand at 4347.304 kb on + strand at 4347.304 kb on + strand at 4347.304 kb on + strand at 4347.304 kb on + strand at 4347.305 kb on - strand at 4347.305 kb on - strand at 4347.305 kb on - strand at 4347.327 kb on + strand at 4347.327 kb on + strand at 4347.327 kb on + strand at 4347.327 kb on + strand at 4347.327 kb on + strand at 4347.328 kb on - strand at 4347.328 kb on - strand at 4347.328 kb on - strand at 4347.328 kb on - strand at 4347.382 kb on - strand at 4347.404 kb on + strand at 4347.404 kb on + strand at 4347.404 kb on + strand at 4347.404 kb on + strand at 4347.405 kb on - strand at 4347.405 kb on - strand at 4347.405 kb on - strand at 4347.405 kb on - strand at 4347.405 kb on - strand at 4347.408 kb on + strand at 4347.408 kb on + strand at 4347.408 kb on + strand at 4347.409 kb on - strand at 4347.409 kb on - strand at 4347.409 kb on - strand at 4347.409 kb on - strand at 4347.414 kb on + strand at 4347.414 kb on + strand at 4347.414 kb on + strand at 4347.414 kb on + strand at 4347.414 kb on + strand at 4347.415 kb on - strand at 4347.415 kb on - strand at 4347.415 kb on - strand at 4347.536 kb on + strand, within RR42_RS20115 at 4347.552 kb on + strand, within RR42_RS20115 at 4347.553 kb on - strand, within RR42_RS20115 at 4347.553 kb on - strand, within RR42_RS20115 at 4347.553 kb on - strand, within RR42_RS20115 at 4347.559 kb on + strand, within RR42_RS20115 at 4347.559 kb on + strand, within RR42_RS20115 at 4347.560 kb on - strand, within RR42_RS20115 at 4347.560 kb on - strand, within RR42_RS20115 at 4347.594 kb on + strand, within RR42_RS20115 at 4347.594 kb on + strand, within RR42_RS20115 at 4347.678 kb on + strand at 4347.678 kb on + strand at 4347.678 kb on + strand at 4347.678 kb on + strand at 4347.678 kb on + strand at 4347.678 kb on + strand at 4347.679 kb on - strand at 4347.679 kb on - strand at 4347.679 kb on - strand at 4347.694 kb on + strand at 4347.695 kb on - strand at 4347.695 kb on - strand at 4347.695 kb on - strand at 4347.991 kb on + strand at 4347.992 kb on - strand at 4347.992 kb on - strand at 4347.996 kb on + strand at 4347.996 kb on + strand at 4347.996 kb on + strand at 4347.996 kb on + strand at 4347.996 kb on + strand at 4347.996 kb on + strand at 4347.997 kb on - strand at 4347.997 kb on - strand at 4347.997 kb on - strand at 4347.997 kb on - strand at 4347.997 kb on - strand at 4348.047 kb on + strand at 4348.047 kb on + strand at 4348.047 kb on + strand at 4348.048 kb on - strand at 4348.048 kb on - strand at 4348.048 kb on - strand at 4348.048 kb on - strand at 4348.089 kb on + strand at 4348.090 kb on - strand at 4348.147 kb on - strand, within RR42_RS20120 at 4348.209 kb on + strand, within RR42_RS20120 at 4348.209 kb on + strand, within RR42_RS20120 at 4348.209 kb on + strand, within RR42_RS20120 at 4348.210 kb on - strand, within RR42_RS20120 at 4348.210 kb on - strand, within RR42_RS20120 at 4348.210 kb on - strand, within RR42_RS20120 at 4348.210 kb on - strand, within RR42_RS20120 at 4348.257 kb on + strand, within RR42_RS20120 at 4348.258 kb on - strand, within RR42_RS20120 at 4348.258 kb on - strand, within RR42_RS20120 at 4348.258 kb on - strand, within RR42_RS20120 at 4348.258 kb on - strand, within RR42_RS20120 at 4348.606 kb on - strand, within RR42_RS20120 at 4348.737 kb on + strand, within RR42_RS20120 at 4348.737 kb on + strand, within RR42_RS20120 at 4348.738 kb on - strand, within RR42_RS20120 at 4348.738 kb on - strand, within RR42_RS20120 at 4348.785 kb on + strand, within RR42_RS20120 at 4348.785 kb on + strand, within RR42_RS20120 at 4348.786 kb on - strand, within RR42_RS20120 at 4348.917 kb on - strand, within RR42_RS20120 at 4348.917 kb on - strand, within RR42_RS20120 at 4349.008 kb on - strand, within RR42_RS20120 at 4349.160 kb on + strand at 4349.160 kb on + strand at 4349.160 kb on + strand at 4349.160 kb on + strand at 4349.161 kb on - strand at 4349.161 kb on - strand at 4349.161 kb on - strand at 4349.164 kb on + strand at 4349.164 kb on + strand at 4349.164 kb on + strand at 4349.164 kb on + strand at 4349.165 kb on - strand at 4349.165 kb on - strand at 4349.165 kb on - strand at 4349.165 kb on - strand at 4349.165 kb on - strand at 4349.165 kb on - strand at 4349.165 kb on - strand at 4349.165 kb on - strand at 4349.171 kb on + strand at 4349.172 kb on - strand at 4349.172 kb on - strand at 4349.172 kb on - strand at 4349.172 kb on - strand at 4349.172 kb on - strand at 4349.172 kb on - strand at 4349.173 kb on + strand at 4349.174 kb on - strand at 4349.236 kb on - strand at 4349.236 kb on - strand at 4349.382 kb on - strand at 4349.451 kb on + strand at 4349.547 kb on + strand at 4349.547 kb on + strand at 4349.547 kb on + strand at 4349.547 kb on + strand at 4349.548 kb on - strand at 4349.548 kb on - strand at 4349.548 kb on - strand at 4349.548 kb on - strand at 4349.625 kb on + strand at 4349.701 kb on + strand, within RR42_RS20125 at 4349.702 kb on - strand, within RR42_RS20125 at 4349.702 kb on - strand, within RR42_RS20125 at 4349.702 kb on - strand, within RR42_RS20125 at 4349.702 kb on - strand, within RR42_RS20125 at 4349.702 kb on - strand, within RR42_RS20125 at 4349.959 kb on + strand, within RR42_RS20125 at 4349.960 kb on - strand, within RR42_RS20125 at 4349.960 kb on - strand, within RR42_RS20125 at 4349.960 kb on - strand, within RR42_RS20125 at 4349.960 kb on - strand, within RR42_RS20125 at 4350.041 kb on + strand, within RR42_RS20125 at 4350.042 kb on - strand, within RR42_RS20125 at 4350.042 kb on - strand, within RR42_RS20125 at 4350.056 kb on - strand, within RR42_RS20125
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.5 mM remove 4,347,159 + +0.9 4,347,179 - -0.1 4,347,179 - -0.5 4,347,238 + +0.0 4,347,238 + +0.5 4,347,238 + +0.5 4,347,239 - -0.8 4,347,239 - -0.1 4,347,294 - -0.7 4,347,304 + -2.6 4,347,304 + +0.2 4,347,304 + -0.6 4,347,304 + +0.3 4,347,305 - -0.1 4,347,305 - -0.1 4,347,305 - -0.9 4,347,327 + +0.2 4,347,327 + +1.2 4,347,327 + +0.9 4,347,327 + -0.5 4,347,327 + +1.6 4,347,328 - +1.0 4,347,328 - +0.1 4,347,328 - -1.6 4,347,328 - +0.3 4,347,382 - +0.6 4,347,404 + +0.4 4,347,404 + -0.6 4,347,404 + -0.5 4,347,404 + +0.1 4,347,405 - -0.1 4,347,405 - -1.0 4,347,405 - -0.3 4,347,405 - +1.2 4,347,405 - -0.5 4,347,408 + +0.0 4,347,408 + +0.1 4,347,408 + +0.8 4,347,409 - +0.0 4,347,409 - -0.1 4,347,409 - -0.8 4,347,409 - -1.2 4,347,414 + +1.1 4,347,414 + -0.6 4,347,414 + -0.2 4,347,414 + -1.3 4,347,414 + -1.2 4,347,415 - +1.1 4,347,415 - +0.1 4,347,415 - -1.0 4,347,536 + RR42_RS20115 0.42 +0.3 4,347,552 + RR42_RS20115 0.49 -1.1 4,347,553 - RR42_RS20115 0.49 -0.1 4,347,553 - RR42_RS20115 0.49 -0.0 4,347,553 - RR42_RS20115 0.49 -0.4 4,347,559 + RR42_RS20115 0.52 +1.5 4,347,559 + RR42_RS20115 0.52 +0.5 4,347,560 - RR42_RS20115 0.52 -1.5 4,347,560 - RR42_RS20115 0.52 -1.0 4,347,594 + RR42_RS20115 0.65 -0.0 4,347,594 + RR42_RS20115 0.65 -0.3 4,347,678 + +1.1 4,347,678 + +0.0 4,347,678 + -0.4 4,347,678 + -0.7 4,347,678 + +1.2 4,347,678 + -0.2 4,347,679 - -0.1 4,347,679 - +0.6 4,347,679 - +0.6 4,347,694 + +0.9 4,347,695 - +0.7 4,347,695 - +0.4 4,347,695 - -2.6 4,347,991 + -1.4 4,347,992 - +0.0 4,347,992 - -1.0 4,347,996 + +2.2 4,347,996 + -0.2 4,347,996 + -1.1 4,347,996 + +0.3 4,347,996 + +0.0 4,347,996 + -0.6 4,347,997 - -2.5 4,347,997 - -2.5 4,347,997 - -2.0 4,347,997 - +0.6 4,347,997 - -0.2 4,348,047 + -3.5 4,348,047 + -1.9 4,348,047 + -0.7 4,348,048 - -1.1 4,348,048 - -0.7 4,348,048 - -2.0 4,348,048 - -0.2 4,348,089 + +0.3 4,348,090 - -1.6 4,348,147 - RR42_RS20120 0.13 -0.7 4,348,209 + RR42_RS20120 0.19 -0.2 4,348,209 + RR42_RS20120 0.19 -1.8 4,348,209 + RR42_RS20120 0.19 +0.4 4,348,210 - RR42_RS20120 0.19 -0.4 4,348,210 - RR42_RS20120 0.19 -1.7 4,348,210 - RR42_RS20120 0.19 -0.4 4,348,210 - RR42_RS20120 0.19 -1.2 4,348,257 + RR42_RS20120 0.23 -0.5 4,348,258 - RR42_RS20120 0.23 -1.5 4,348,258 - RR42_RS20120 0.23 +0.0 4,348,258 - RR42_RS20120 0.23 +0.2 4,348,258 - RR42_RS20120 0.23 -2.1 4,348,606 - RR42_RS20120 0.53 -0.5 4,348,737 + RR42_RS20120 0.64 +1.0 4,348,737 + RR42_RS20120 0.64 -0.1 4,348,738 - RR42_RS20120 0.64 +0.1 4,348,738 - RR42_RS20120 0.64 -0.5 4,348,785 + RR42_RS20120 0.69 -0.3 4,348,785 + RR42_RS20120 0.69 -1.0 4,348,786 - RR42_RS20120 0.69 -0.6 4,348,917 - RR42_RS20120 0.80 -0.7 4,348,917 - RR42_RS20120 0.80 -1.1 4,349,008 - RR42_RS20120 0.88 +0.0 4,349,160 + -0.5 4,349,160 + -0.5 4,349,160 + +0.8 4,349,160 + -0.4 4,349,161 - +1.8 4,349,161 - +1.3 4,349,161 - -1.7 4,349,164 + -1.3 4,349,164 + -0.8 4,349,164 + +1.3 4,349,164 + -0.0 4,349,165 - -0.8 4,349,165 - -0.6 4,349,165 - -0.2 4,349,165 - +0.4 4,349,165 - +0.3 4,349,165 - -0.1 4,349,165 - -0.8 4,349,165 - -0.7 4,349,171 + -3.4 4,349,172 - -1.4 4,349,172 - -0.9 4,349,172 - +0.8 4,349,172 - +0.0 4,349,172 - +0.0 4,349,172 - -1.1 4,349,173 + -1.9 4,349,174 - +0.4 4,349,236 - +0.1 4,349,236 - -2.4 4,349,382 - -0.9 4,349,451 + -0.3 4,349,547 + +0.4 4,349,547 + +1.0 4,349,547 + +0.5 4,349,547 + -0.0 4,349,548 - +1.9 4,349,548 - +0.2 4,349,548 - +1.4 4,349,548 - +1.3 4,349,625 + +1.0 4,349,701 + RR42_RS20125 0.10 -0.4 4,349,702 - RR42_RS20125 0.10 -0.0 4,349,702 - RR42_RS20125 0.10 +0.2 4,349,702 - RR42_RS20125 0.10 +0.4 4,349,702 - RR42_RS20125 0.10 +0.5 4,349,702 - RR42_RS20125 0.10 +0.1 4,349,959 + RR42_RS20125 0.17 -0.6 4,349,960 - RR42_RS20125 0.17 -0.8 4,349,960 - RR42_RS20125 0.17 -0.5 4,349,960 - RR42_RS20125 0.17 -0.7 4,349,960 - RR42_RS20125 0.17 +0.8 4,350,041 + RR42_RS20125 0.19 -1.0 4,350,042 - RR42_RS20125 0.19 +0.1 4,350,042 - RR42_RS20125 0.19 -0.3 4,350,056 - RR42_RS20125 0.19 -0.2
Or see this region's nucleotide sequence