Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS16235

Experiment: R2A with Nickel (II) chloride 0.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS16230 and RR42_RS16235 overlap by 4 nucleotidesRR42_RS16235 and RR42_RS16240 are separated by 44 nucleotidesRR42_RS16240 and RR42_RS16245 are separated by 190 nucleotides RR42_RS16230: RR42_RS16230 - permease, at 3,513,735 to 3,514,859 _RS16230 RR42_RS16235: RR42_RS16235 - permease, at 3,514,856 to 3,516,010 _RS16235 RR42_RS16240: RR42_RS16240 - cobalamin biosynthesis protein CbiX, at 3,516,055 to 3,516,459 _RS16240 RR42_RS16245: RR42_RS16245 - uroporphyrin-III methyltransferase, at 3,516,650 to 3,517,420 _RS16245 Position (kb) 3514 3515 3516 3517Strain fitness (log2 ratio) -2 -1 0 1 2at 3516.009 kb on - strandat 3516.009 kb on - strandat 3516.060 kb on + strandat 3516.060 kb on + strandat 3516.061 kb on - strandat 3516.418 kb on + strand, within RR42_RS16240at 3516.418 kb on + strand, within RR42_RS16240at 3516.614 kb on + strandat 3516.615 kb on - strandat 3516.632 kb on + strandat 3516.633 kb on - strandat 3516.633 kb on - strandat 3516.672 kb on + strandat 3516.673 kb on - strandat 3516.673 kb on - strandat 3516.673 kb on - strandat 3516.930 kb on + strand, within RR42_RS16245at 3516.930 kb on + strand, within RR42_RS16245at 3516.930 kb on + strand, within RR42_RS16245at 3516.930 kb on + strand, within RR42_RS16245at 3516.931 kb on - strand, within RR42_RS16245at 3516.931 kb on - strand, within RR42_RS16245at 3516.931 kb on - strand, within RR42_RS16245at 3516.933 kb on + strand, within RR42_RS16245at 3516.933 kb on + strand, within RR42_RS16245at 3516.944 kb on - strand, within RR42_RS16245

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.5 mM
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3,516,009 - +0.1
3,516,009 - +0.6
3,516,060 + -0.6
3,516,060 + -1.4
3,516,061 - -0.2
3,516,418 + RR42_RS16240 0.90 +0.3
3,516,418 + RR42_RS16240 0.90 -1.3
3,516,614 + +0.1
3,516,615 - +0.6
3,516,632 + +0.5
3,516,633 - -0.5
3,516,633 - +0.4
3,516,672 + -0.1
3,516,673 - +0.3
3,516,673 - +0.4
3,516,673 - -0.9
3,516,930 + RR42_RS16245 0.36 -1.5
3,516,930 + RR42_RS16245 0.36 -0.5
3,516,930 + RR42_RS16245 0.36 -0.1
3,516,930 + RR42_RS16245 0.36 -0.6
3,516,931 - RR42_RS16245 0.36 -0.3
3,516,931 - RR42_RS16245 0.36 +0.1
3,516,931 - RR42_RS16245 0.36 -0.4
3,516,933 + RR42_RS16245 0.37 -2.3
3,516,933 + RR42_RS16245 0.37 -1.0
3,516,944 - RR42_RS16245 0.38 +1.9

Or see this region's nucleotide sequence