Experiment: R2A with Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS13980 and RR42_RS13985 are separated by 104 nucleotides RR42_RS13985 and RR42_RS13990 are separated by 79 nucleotides RR42_RS13990 and RR42_RS13995 are separated by 167 nucleotides RR42_RS13995 and RR42_RS14000 are separated by 103 nucleotides
RR42_RS13980: RR42_RS13980 - tRNA-Cys, at 3,012,643 to 3,012,716
_RS13980
RR42_RS13985: RR42_RS13985 - tRNA-Gly, at 3,012,821 to 3,012,896
_RS13985
RR42_RS13990: RR42_RS13990 - tRNA-Gly, at 3,012,976 to 3,013,051
_RS13990
RR42_RS13995: RR42_RS13995 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 3,013,219 to 3,013,821
_RS13995
RR42_RS14000: RR42_RS14000 - excinuclease ABC subunit C, at 3,013,925 to 3,015,985
_RS14000
Position (kb)
3013
3014 Strain fitness (log2 ratio)
-2
-1
0
1 at 3012.249 kb on + strand at 3012.250 kb on - strand at 3012.250 kb on - strand at 3012.616 kb on - strand at 3012.641 kb on - strand at 3012.641 kb on - strand at 3012.641 kb on - strand at 3013.097 kb on - strand at 3013.203 kb on - strand at 3013.932 kb on - strand at 3013.932 kb on - strand at 3013.932 kb on - strand at 3013.942 kb on - strand at 3014.287 kb on + strand, within RR42_RS14000 at 3014.499 kb on + strand, within RR42_RS14000 at 3014.500 kb on - strand, within RR42_RS14000 at 3014.500 kb on - strand, within RR42_RS14000 at 3014.533 kb on - strand, within RR42_RS14000 at 3014.533 kb on - strand, within RR42_RS14000 at 3014.533 kb on - strand, within RR42_RS14000
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.5 mM remove 3,012,249 + -0.8 3,012,250 - +0.4 3,012,250 - -0.3 3,012,616 - +0.4 3,012,641 - -0.3 3,012,641 - -1.6 3,012,641 - -0.1 3,013,097 - -2.6 3,013,203 - +0.0 3,013,932 - +0.5 3,013,932 - -0.0 3,013,932 - -0.1 3,013,942 - -0.8 3,014,287 + RR42_RS14000 0.18 -0.2 3,014,499 + RR42_RS14000 0.28 -1.0 3,014,500 - RR42_RS14000 0.28 +0.1 3,014,500 - RR42_RS14000 0.28 +0.5 3,014,533 - RR42_RS14000 0.30 -1.1 3,014,533 - RR42_RS14000 0.30 -1.2 3,014,533 - RR42_RS14000 0.30 +0.3
Or see this region's nucleotide sequence