Experiment: R2A with Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS13355 and RR42_RS13360 are separated by 248 nucleotides RR42_RS13360 and RR42_RS13365 are separated by 80 nucleotides
RR42_RS13355: RR42_RS13355 - glutamyl-tRNA synthetase, at 2,879,687 to 2,881,093
_RS13355
RR42_RS13360: RR42_RS13360 - esterase, at 2,881,342 to 2,882,292
_RS13360
RR42_RS13365: RR42_RS13365 - peptidase P60, at 2,882,373 to 2,883,059
_RS13365
Position (kb)
2881
2882
2883 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2881.145 kb on - strand at 2881.159 kb on - strand at 2881.160 kb on + strand at 2881.160 kb on + strand at 2881.160 kb on + strand at 2881.161 kb on - strand at 2881.161 kb on - strand at 2881.224 kb on + strand at 2881.224 kb on + strand at 2881.225 kb on - strand at 2881.225 kb on - strand at 2881.225 kb on - strand at 2881.225 kb on - strand at 2881.263 kb on + strand at 2881.264 kb on - strand at 2881.264 kb on - strand at 2881.267 kb on + strand at 2881.621 kb on + strand, within RR42_RS13360 at 2881.621 kb on + strand, within RR42_RS13360 at 2881.753 kb on + strand, within RR42_RS13360 at 2881.753 kb on + strand, within RR42_RS13360 at 2881.753 kb on + strand, within RR42_RS13360 at 2881.753 kb on + strand, within RR42_RS13360 at 2881.753 kb on + strand, within RR42_RS13360 at 2881.754 kb on - strand, within RR42_RS13360 at 2881.754 kb on - strand, within RR42_RS13360 at 2881.754 kb on - strand, within RR42_RS13360 at 2881.890 kb on + strand, within RR42_RS13360 at 2881.936 kb on + strand, within RR42_RS13360 at 2881.936 kb on + strand, within RR42_RS13360 at 2881.936 kb on + strand, within RR42_RS13360 at 2881.936 kb on + strand, within RR42_RS13360 at 2881.937 kb on - strand, within RR42_RS13360 at 2881.937 kb on - strand, within RR42_RS13360 at 2881.937 kb on - strand, within RR42_RS13360 at 2882.017 kb on - strand, within RR42_RS13360 at 2882.064 kb on - strand, within RR42_RS13360 at 2882.435 kb on + strand at 2882.436 kb on - strand at 2882.436 kb on - strand at 2882.501 kb on - strand, within RR42_RS13365 at 2882.591 kb on - strand, within RR42_RS13365 at 2882.741 kb on - strand, within RR42_RS13365 at 2882.992 kb on - strand at 2882.992 kb on - strand at 2883.183 kb on - strand at 2883.183 kb on - strand at 2883.210 kb on + strand at 2883.210 kb on + strand at 2883.227 kb on + strand at 2883.228 kb on - strand at 2883.229 kb on + strand at 2883.229 kb on + strand at 2883.229 kb on + strand at 2883.229 kb on + strand at 2883.230 kb on - strand at 2883.230 kb on - strand at 2883.230 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.5 mM remove 2,881,145 - +1.5 2,881,159 - +0.4 2,881,160 + +1.1 2,881,160 + +0.2 2,881,160 + -1.8 2,881,161 - -0.1 2,881,161 - -0.2 2,881,224 + +1.2 2,881,224 + -0.3 2,881,225 - +0.1 2,881,225 - -0.3 2,881,225 - -2.0 2,881,225 - -0.8 2,881,263 + +1.7 2,881,264 - -2.8 2,881,264 - -3.2 2,881,267 + -1.1 2,881,621 + RR42_RS13360 0.29 -0.6 2,881,621 + RR42_RS13360 0.29 -5.0 2,881,753 + RR42_RS13360 0.43 -4.1 2,881,753 + RR42_RS13360 0.43 -0.8 2,881,753 + RR42_RS13360 0.43 -3.1 2,881,753 + RR42_RS13360 0.43 -1.9 2,881,753 + RR42_RS13360 0.43 -3.3 2,881,754 - RR42_RS13360 0.43 -1.1 2,881,754 - RR42_RS13360 0.43 -1.8 2,881,754 - RR42_RS13360 0.43 -0.5 2,881,890 + RR42_RS13360 0.58 -1.6 2,881,936 + RR42_RS13360 0.62 -4.6 2,881,936 + RR42_RS13360 0.62 -0.5 2,881,936 + RR42_RS13360 0.62 -1.2 2,881,936 + RR42_RS13360 0.62 +0.4 2,881,937 - RR42_RS13360 0.63 -1.3 2,881,937 - RR42_RS13360 0.63 +0.6 2,881,937 - RR42_RS13360 0.63 -3.0 2,882,017 - RR42_RS13360 0.71 -0.1 2,882,064 - RR42_RS13360 0.76 -0.9 2,882,435 + -0.1 2,882,436 - +0.6 2,882,436 - -0.6 2,882,501 - RR42_RS13365 0.19 -2.5 2,882,591 - RR42_RS13365 0.32 -0.3 2,882,741 - RR42_RS13365 0.54 -3.6 2,882,992 - -2.6 2,882,992 - -0.8 2,883,183 - +0.4 2,883,183 - -1.1 2,883,210 + -2.8 2,883,210 + -2.4 2,883,227 + -2.4 2,883,228 - +0.3 2,883,229 + +0.1 2,883,229 + -3.5 2,883,229 + -2.3 2,883,229 + -4.7 2,883,230 - -0.6 2,883,230 - +0.4 2,883,230 - +0.3
Or see this region's nucleotide sequence