Experiment: R2A with Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11975 and RR42_RS11980 overlap by 23 nucleotides RR42_RS11980 and RR42_RS11985 are separated by 11 nucleotides RR42_RS11985 and RR42_RS11990 are separated by 155 nucleotides
RR42_RS11975: RR42_RS11975 - hypothetical protein, at 2,565,408 to 2,566,475
_RS11975
RR42_RS11980: RR42_RS11980 - glutathione ABC transporter permease, at 2,566,453 to 2,567,370
_RS11980
RR42_RS11985: RR42_RS11985 - glutathione ABC transporter permease, at 2,567,382 to 2,568,302
_RS11985
RR42_RS11990: RR42_RS11990 - glutathione ABC transporter substrate-binding protein, at 2,568,458 to 2,570,005
_RS11990
Position (kb)
2567
2568
2569 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2566.694 kb on + strand, within RR42_RS11980 at 2566.784 kb on + strand, within RR42_RS11980 at 2566.784 kb on + strand, within RR42_RS11980 at 2566.785 kb on - strand, within RR42_RS11980 at 2566.809 kb on + strand, within RR42_RS11980 at 2566.810 kb on - strand, within RR42_RS11980 at 2566.983 kb on - strand, within RR42_RS11980 at 2566.983 kb on - strand, within RR42_RS11980 at 2566.983 kb on - strand, within RR42_RS11980 at 2567.181 kb on - strand, within RR42_RS11980 at 2567.359 kb on + strand at 2567.384 kb on - strand at 2567.384 kb on - strand at 2567.384 kb on - strand at 2567.388 kb on - strand at 2567.389 kb on + strand at 2567.390 kb on - strand at 2567.785 kb on + strand, within RR42_RS11985 at 2567.785 kb on + strand, within RR42_RS11985 at 2567.785 kb on + strand, within RR42_RS11985 at 2567.786 kb on - strand, within RR42_RS11985 at 2567.799 kb on - strand, within RR42_RS11985 at 2568.318 kb on + strand at 2568.460 kb on - strand at 2568.511 kb on - strand at 2568.540 kb on + strand at 2568.540 kb on + strand at 2568.540 kb on + strand at 2568.541 kb on - strand at 2568.541 kb on - strand at 2568.609 kb on + strand at 2568.641 kb on - strand, within RR42_RS11990 at 2568.685 kb on - strand, within RR42_RS11990 at 2568.685 kb on - strand, within RR42_RS11990 at 2568.780 kb on - strand, within RR42_RS11990 at 2568.780 kb on - strand, within RR42_RS11990 at 2568.780 kb on - strand, within RR42_RS11990 at 2568.783 kb on + strand, within RR42_RS11990 at 2568.783 kb on + strand, within RR42_RS11990 at 2568.783 kb on + strand, within RR42_RS11990 at 2568.783 kb on + strand, within RR42_RS11990 at 2568.784 kb on - strand, within RR42_RS11990 at 2568.784 kb on - strand, within RR42_RS11990 at 2568.784 kb on - strand, within RR42_RS11990 at 2568.786 kb on + strand, within RR42_RS11990 at 2568.787 kb on - strand, within RR42_RS11990 at 2568.787 kb on - strand, within RR42_RS11990 at 2568.854 kb on + strand, within RR42_RS11990 at 2568.940 kb on - strand, within RR42_RS11990 at 2569.018 kb on - strand, within RR42_RS11990 at 2569.018 kb on - strand, within RR42_RS11990 at 2569.018 kb on - strand, within RR42_RS11990 at 2569.131 kb on + strand, within RR42_RS11990 at 2569.131 kb on + strand, within RR42_RS11990 at 2569.132 kb on - strand, within RR42_RS11990
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.5 mM remove 2,566,694 + RR42_RS11980 0.26 +1.5 2,566,784 + RR42_RS11980 0.36 -0.1 2,566,784 + RR42_RS11980 0.36 +0.4 2,566,785 - RR42_RS11980 0.36 +1.2 2,566,809 + RR42_RS11980 0.39 +0.3 2,566,810 - RR42_RS11980 0.39 +0.1 2,566,983 - RR42_RS11980 0.58 +0.6 2,566,983 - RR42_RS11980 0.58 -2.0 2,566,983 - RR42_RS11980 0.58 -1.5 2,567,181 - RR42_RS11980 0.79 -0.2 2,567,359 + +1.1 2,567,384 - -2.4 2,567,384 - -0.5 2,567,384 - -0.4 2,567,388 - -0.4 2,567,389 + -1.5 2,567,390 - +0.2 2,567,785 + RR42_RS11985 0.44 -0.2 2,567,785 + RR42_RS11985 0.44 +1.3 2,567,785 + RR42_RS11985 0.44 +0.9 2,567,786 - RR42_RS11985 0.44 +0.0 2,567,799 - RR42_RS11985 0.45 -0.7 2,568,318 + +0.3 2,568,460 - +0.2 2,568,511 - +0.0 2,568,540 + +0.5 2,568,540 + -0.8 2,568,540 + +0.3 2,568,541 - -0.4 2,568,541 - -0.7 2,568,609 + -0.1 2,568,641 - RR42_RS11990 0.12 -0.4 2,568,685 - RR42_RS11990 0.15 +0.2 2,568,685 - RR42_RS11990 0.15 +0.7 2,568,780 - RR42_RS11990 0.21 -0.1 2,568,780 - RR42_RS11990 0.21 -0.7 2,568,780 - RR42_RS11990 0.21 -2.8 2,568,783 + RR42_RS11990 0.21 -1.8 2,568,783 + RR42_RS11990 0.21 +1.4 2,568,783 + RR42_RS11990 0.21 +0.5 2,568,783 + RR42_RS11990 0.21 -1.8 2,568,784 - RR42_RS11990 0.21 +0.5 2,568,784 - RR42_RS11990 0.21 +0.4 2,568,784 - RR42_RS11990 0.21 -0.8 2,568,786 + RR42_RS11990 0.21 -1.0 2,568,787 - RR42_RS11990 0.21 +0.4 2,568,787 - RR42_RS11990 0.21 -0.2 2,568,854 + RR42_RS11990 0.26 -0.2 2,568,940 - RR42_RS11990 0.31 -2.8 2,569,018 - RR42_RS11990 0.36 +0.8 2,569,018 - RR42_RS11990 0.36 +1.3 2,569,018 - RR42_RS11990 0.36 +0.6 2,569,131 + RR42_RS11990 0.43 +0.9 2,569,131 + RR42_RS11990 0.43 -0.1 2,569,132 - RR42_RS11990 0.44 +0.6
Or see this region's nucleotide sequence